Scoparone

別名：6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone

製品コード：S3613 純度：99.65%

Scoparone (6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone) is a natural organic compound discovered from Chinese herb Artemisia scoparia with anticoagulant, hypolipidemic, vasorelaxant, antioxidant, and anti-inflammatory actions.

CAS No. 120-08-1

サイズ	価格(税別)	在庫状況
5mg	JPY 15900	国内在庫あり
25mg	JPY 39000	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

製品安全説明書

現在のバッチを見る： S361301 DMSO] 41 mg/mL] false] Ethanol] 41 mg/mL] false] Water] Insoluble] false 純度： 99.65%

99.65

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関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Express-Pick Library	もっと見る

生物活性

製品説明	Scoparone (6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone) is a natural organic compound discovered from Chinese herb Artemisia scoparia with anticoagulant, hypolipidemic, vasorelaxant, antioxidant, and anti-inflammatory actions.

In Vitro
In vitro	The proliferation of VSMCs is significantly attenuated by scoparone in a dose-dependent manner. Scoparone markedly reduces the serum-stimulated accumulation of cells in the S phase and concomitantly increases the proportion of cells in the G0/G1 phase, which is consistent with the reduced expression of cyclin D1, phosphorylated Rb and survivin in the VSMCs. Cell adhesion markers, such as MCP-1 and ICAM-1, are significantly reduced by scoparone. It attenuates the increase in cyclin D promoter activity by inhibiting the activities of both the WT and active forms of STAT3. scoparone blocks the accumulation of STAT3 transported from the cytosol to the nucleus, leading to the suppression of VSMC proliferation through G1 phase arrest and the inhibition of Rb phosphorylation. This activity occurs independent of the form of STAT3 and upstream of kinases, such as Jak and Src, which are correlated with abnormal vascular remodeling due to the presence of an excess of growth factors following vascular injury. The protective effect of scoparone involves anti-inflammatory functions that occur via the inhibition of NF-κB activity, leading to the downregulation of pro-inflammatory cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1^[1].
細胞実験	細胞株	rat VSMCs
	濃度	500 μM
	反応時間	24 h
	実験の流れ	The rat VSMCs are seeded in six-well plates at 90% confluence and are starved in serum-free media for 24 h followed by incubation in 1% FBS for an additional 24 h. The cells in the wells are detached by razor scraping, rinsed with PBS, stimulated in media containing 20% FBS, co-treated with 0.2% DMSO or 500 μM scoparone for 24 h and then stained with hematoxylin. Cell images are obtained via microscopy.

In Vivo
In Vivo	The free radical scavenging property of scoparone leads to the attenuation of intima thickening and improves hemodynamics in cholesterol-fed alloxan-diabetic rabbits concurrent with decreases in plasma lipid and lipoprotein cholesterol concentrations^[1]. Scoparone exhibited therapeutic efficacies on liver damage in vivo^[2].
動物実験	動物モデル	Male Wistar rats
	投与量	0.7 mg/kg
	投与経路	oral

参考
[1] Park S, et al. Exp Mol Med. 2015, 47:e145. [2] Zhang A, et al. Sci Rep. 2014, 4:6768.

参考

化学情報

分子量	206.19	化学式	C₁₁H₁₀O₄
CAS No.	120-08-1	SDF	Download Scoparone SDFをダウンロードする
Smiles	COC1=C(C=C2C(=C1)C=CC(=O)O2)OC
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

DMSO : 41 mg/mL ( (198.84 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 41 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

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