Triolein

Triolein is an inhibitor of metalloproteinase-1 (MMP-1) and reduces both the expression of IL-6 and ROS generation in irradiated keratinocytes, which can be proposed as an active agent against skin photodamage.^[1]

Tel‐E6E7 cells were seeded on day one at a density of 2000 cells per cm² on 3T3‐swiss cells feeder layer in cFAD medium. On day five the medium was change to DMEM and F12 (1:1) mixture containing 2 mM glutamine, hydrocortisone (0.4 µg/ml), 2 mM penicillin/streptomycin, Epithelial Growth factor (5 ng/ml) and 10 % fetal bovine serum. On day six the cells were irradiated on PBS using a UVM‐57 UVP handheld UV lamp (302 nm Midrange) at sub‐lethal doses of 3.4 or 6.8 mJ/cm². Immediately, PBS was replaced with medium containing triolein 20 µM. 24 hours later mRNA was extracted from cells using Trizol Reagent. Conditioned culture medium was collected and used to replace the medium of dermal fibroblasts cells plated the day before (75000 cells/cm²) in DMEM supplemented with 10% fetal bovine serum and 2 mM glutamine. After 48 hours dermal fibroblasts mRNA was extracted.