MCF7 |
Cell cycle assay |
4 uM |
14 days |
Induction of cell cycle arrest in human MCF7 cells transfected with p53-targeting iRNA-based shRNA at 4 uM after 14 days by BrdU incorporation assay |
16474381 |
MCF7 |
Cell cycle assay |
4 uM |
14 days |
Induction of cell cycle arrest in human MCF7 cells transfected hnRNPK-targeting iRNA-based shRNA at 4 uM after 14 days by BrdU incorporation assay |
16474381 |
MCF7 |
Antiproliferative assay |
4 uM |
14 days |
Antiproliferative activity against human MCF7 cells at 4 uM after 14 days by coomassie staining |
16474381 |
MCF7 |
Antiproliferative assay |
4 uM |
14 days |
Antiproliferative activity against human MCF7 cells assessed as appearance of flattened phenotype at 4 uM after 14 days by coomassie staining |
16474381 |
MCF7 |
Cell cycle assay |
4 uM |
14 days |
Induction of cell cycle arrest in human MCF7 cells transfected with 53BP1-targeting iRNA-based shRNA at 4 uM after 14 days by BrdU incorporation assay |
16474381 |
SNJSA1 |
Function assay |
1 to 10 uM |
6 hrs |
Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 10 uM after 6 hrs by Western blot analysis |
21314128 |
SNJSA1 |
Function assay |
1 to 10 uM |
6 hrs |
Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 10 uM after 6 hrs by Western blot analysis |
21314128 |
SNJSA1 |
Function assay |
1 to 10 uM |
6 hrs |
Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 10 uM after 6 hrs by Western blot analysis |
21314128 |
HCT116 |
Function assay |
50 uM |
24 hrs |
Inhibition of p53-MDM2 (unknown origin) interaction expressed in p53-deficient human HCT116 cells at 50 uM after 24 hrs by BiFC-based FACS flow cytometric analysis |
24268795 |
U2OS |
Function assay |
2.5 uM |
12 hrs |
Stabilization of p53 in human U2OS cells assessed as intracellular p53 level at 2.5 uM after 12 hrs by Western blotting |
26288684 |
HCT116 |
Function assay |
10 uM |
20 hrs |
Inhibition of V1-p53/MDM2-V2 (unknown origin) interaction expressed in human HCT116 p53-/- cells at 10 uM after 20 hrs by biomolecular fluorescence complementation-based flow cytometric analysis |
28800455 |
MCF7 |
Function assay |
10 uM |
16 hrs |
Inhibition of MDM2-p53 interaction in human MCF7 cells assessed as reversal of MDM2-dependent inhibition of p53-induced transcriptional activity at 10 uM after 16 hrs by dual-luciferase reporter gene assay |
23540934 |
MCF7 |
Apoptosis assay |
3 uM |
24 to 72 hrs |
Induction of apoptosis in human MCF7 cells assessed as reduction in Bcl-xL expression level at 3 uM after 24 to 72 hrs by Western blotting analysis |
23802716 |
MCF7 |
Apoptosis assay |
3 uM |
24 to 72 hrs |
Induction of apoptosis in human MCF7 cells assessed as early apoptotic cells at 3 uM after 24 to 72 hrs by annexin V-FITC/propidium iodide staining based FACS flow cytometry (Rvb = 4 %) |
23802716 |
MCF7 |
Apoptosis assay |
3 uM |
24 to 72 hrs |
Induction of apoptosis in human MCF7 cells assessed as increase in p21 expression level at 3 uM after 24 to 72 hrs by Western blotting analysis |
23802716 |
MCF7 |
Apoptosis assay |
3 uM |
24 to 72 hrs |
Induction of apoptosis in human MCF7 cells assessed as increase in Bcl-xS expression level at 3 uM after 24 to 72 hrs by Western blotting analysis |
23802716 |
MCF7 |
Apoptosis assay |
50 nM |
24 to 72 hrs |
Induction of apoptosis in human MCF7 cells assessed as accumulation of caspase-3 cleavage product at 50 nM after 24 to 72 hrs by Western blotting analysis |
23802716 |
MCF7 |
Cell cycle assay |
3 uM |
24 to 72 hrs |
Cell cycle arrest in human MCF7 cells assessed as accumulation at G0/G1 phase at 3 uM after 24 to 72 hrs by propidium iodide staining-based FACS analysis |
23802716 |
SJSA1 |
Function assay |
10 uM |
|
Inhibition of MDM2-p53 interaction in human SJSA1 cells assessed as p53 activation at 10 uM by Western blot |
19928922 |
BJ |
Function assay |
3 uM |
|
Induction of p21CIP1 expression in irradiated human BJ cells at 3 uM by Western blot analysis |
16474381 |
BJ |
Function assay |
3 uM |
|
Induction of p21 expression in irradiated human BJ cells at 3 uM by Western blot analysis |
16474381 |
SJSA1 |
Antiproliferative assay |
|
3 days |
Antiproliferative activity against human SJSA1 cells after 3 days by EdU incorporation assay in presence of 10% human serum, IC50 = 0.7 μM. |
22524527 |
HCT116 |
Function assay |
|
24 hrs |
Induction of p53-Ser15 phosphorylation in human HCT116 cells after 24 hrs, IC50 = 1 μM. |
19856920 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human HCT116 cells expressing wild type p53 incubated for 72 hrs by MTS assay, IC50 = 1.3 μM. |
30221935 |
HCT116 |
Function assay |
|
8 hrs |
Induction of p53 in human HCT116 cells coexpressing pp53TA-luc assessed as inhibition of cell proliferation after 8 hrs by firefly/renilla luciferase reporter assay, IC50 = 1.39 μM. |
19856920 |
HGF |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human HGF cells after 24 hrs by MTT assay, IC50 = 1.4 μM. |
23802716 |
SJSA1 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human SJSA1 cells expressing wild type p53 incubated for 72 hrs by MTS assay, IC50 = 1.44 μM. |
30221935 |
SJSA1 |
Cytotoxicity assay |
|
5 days |
Cytotoxicity against human SJSA1 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay, IC50 = 1.5 μM. |
24900694 |
RKO |
Cytotoxicity assay |
|
5 days |
Cytotoxicity against human RKO cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay, IC50 = 1.5 μM. |
24900694 |
HCT116 |
Cytotoxicity assay |
|
5 days |
Cytotoxicity against human HCT116 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay, IC50 = 1.5 μM. |
24900694 |
HCT116 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human HCT116 cells after 72 hrs by CellTiterGlo luciferase-based assay, IC50 = 1.6 μM. |
24139845 |
SJSA1 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human SJSA1 cells expressing wild type p53 after 72 hrs by CellTitre-Glo assay, IC50 = 1.9 μM. |
26985323 |
NGP |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human NGP cells after 72 hrs by sulforhodamine B assay, GI50 = 2.1 μM. |
21314128 |
LS |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human LS cells after 72 hrs by sulforhodamine B assay, GI50 = 2.3 μM. |
21314128 |
SJSA1 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human SJSA1 cells after 72 hrs by sulforhodamine B assay, GI50 = 2.6 μM. |
21314128 |
MCF7 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay, IC50 = 2.9 μM. |
23802716 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity in p53 (+/+) human HCT116 cells incubated for 72 hrs by MTS assay, IC50 = 4 μM. |
28987608 |
A549 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human A549 cells expressing wild type p53 assessed as growth inhibition after 72 hrs by SRB assay, IC50 = 4.62 μM. |
23601819 |
A549 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human A549 cells expressing wild type p53 after 72 hrs by SRB assay, IC50 = 4.62 μM. |
23611770 |
MCF7 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50 = 5.01 μM. |
27101893 |
HepG2 |
Cytotoxicity assay |
|
48 hrs |
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50 = 5.1 μM. |
25479770 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human HCT116 cells expressing wild type p53 assessed as growth inhibition after 72 hrs by MTT assay, IC50 = 5.21 μM. |
27101893 |
UKF-NB-3 |
Cytotoxicity assay |
|
96 hrs |
Cytotoxicity against doxorubicin-resistant human UKF-NB-3 cells after 96 hrs by MTT assay, IC50 = 5.56 μM. |
20947617 |
SJSA1 |
Function assay |
|
7 hrs |
Activity at p53 in human SJSA1 cells assessed as induction of p21 mRNA expression after 7 hrs by qRT-PCR analysis in presence of 10% human serum, IC50 = 7.1 μM. |
22524527 |
HCT116 |
Growth inhibition assay |
|
48 hrs |
Growth inhibition of human HCT116 cells harboring p53+/+ after 48 hrs by Hoechst 33258 staining based fluorescence assay, GI50 = 8 μM. |
29089230 |
MCF7 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human MCF7 cells after 72 hrs by CCK8 assay, IC50 = 8.5 μM. |
30045621 |
HCT116 |
Antiproliferative assay |
|
24 hrs |
Antiproliferative activity against human HCT116 p53+/+ cells assessed as growth inhibition after 24 hrs by EdU/Hoechst 33342 staining-based fluorescence analysis, GI50 = 9 μM. |
29691156 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human HCT116 cells after 72 hrs by CCK8 assay, IC50 = 9.07 μM. |
30045621 |
A549 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human A549 cells after 72 hrs by CCK8 assay, IC50 = 9.58 μM. |
30045621 |
HepG2 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50 = 10.2 μM. |
23802716 |
NCI-H1299 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against p53 -/- human NCI-H1299 cells incubated for 72 hrs by MTS assay, IC50 = 10.4 μM. |
30221935 |
MCF7 |
Cytotoxicity assay |
|
48 hrs |
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay, IC50 = 11.6 μM. |
25618595 |
Saos2 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human p53-deficient Saos2 cells after 72 hrs by MTT assay, IC50 = 12.1 μM. |
22940704 |
HCT116 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human p53 expressing HCT116 cells after 72 hrs by sulforhodamine B assay, GI50 = 13 μM. |
21314128 |
U2OS |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human U2OS cells expressing wild type p53 after 72 hrs by MTT assay, IC50 = 14.31 μM. |
23046248 |
H1299 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human H1299 cells after 72 hrs by CellTiterGlo luciferase-based assay, IC50 = 14.7 μM. |
24139845 |
A549 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human A549 cells expressing wild type p53 after 72 hrs by MTT assay, IC50 = 15 μM. |
22940704 |
A549 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human A549 cells expressing wild type p53 after 72 hrs by MTT assay, IC50 = 15.12 μM. |
23046248 |
U937 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human U937 cells after 24 hrs by MTT assay, IC50 = 15.6 μM. |
23802716 |
Saos2 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human Saos2 cells after 72 hrs by sulforhodamine B assay, GI50 = 18 μM. |
21314128 |
U2OS |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human U2OS cells expressing wild type p53 after 72 hrs by MTT assay, IC50 = 19.6 μM. |
22940704 |
NCI-H1299 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human NCI-H1299 cells after 72 hrs by CCK8 assay, IC50 = 19.76 μM. |
30045621 |
PC3 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human p53-null PC3 cells assessed as growth inhibition after 72 hrs by SRB assay, IC50 = 20.04 μM. |
23601819 |
PC3 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against human p53 deficient PC3 cells after 72 hrs by SRB assay, IC50 = 20.04 μM. |
23611770 |
NCI-H1299 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human p53-deficient NCI-H1299 cells after 72 hrs by MTT assay, IC50 = 20.4 μM. |
22940704 |
NCI-H1299 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against p53 deficient human NCI-H1299 cells after 72 hrs by MTT assay, IC50 = 20.48 μM. |
23046248 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human p53-null HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50 = 21.5 μM. |
27101893 |
SJSA1 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity in human SJSA1 cells over expressing human DM2 after 24 hrs by CellTiter-Glo assay, EC50 = 22.8 μM. |
29150077 |
C643 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human C643 cells after 24 hrs by MTT assay, IC50 = 23 μM. |
23802716 |
MDA-MB-231 |
Cytotoxicity assay |
|
48 hrs |
Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 23.5 μM. |
25618595 |
U2OS |
Antitumor assay |
|
72 hrs |
Antitumor activity against human U2OS cells expressing wild type p53 after 72 hrs by MTT assay, IC50 = 24.61 μM. |
21996465 |
HCT116 |
Growth inhibition assay |
|
48 hrs |
Growth inhibition of human p53 knockdown HCT116 cells after 48 hrs by Hoechst 33258 staining based fluorescence assay, GI50 = 27 μM. |
29089230 |
Calu |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human Calu cells after 24 hrs by MTT assay, IC50 = 27.2 μM. |
23802716 |
HCT116 |
Antiproliferative assay |
|
24 hrs |
Antiproliferative activity against human HCT116 p53-/- cells assessed as growth inhibition after 24 hrs by EdU/Hoechst 33342 staining-based fluorescence analysis, GI50 = 29 μM. |
29691156 |
MDA-MB-435S |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against human MDA-MB-435S cells assessed as growth inhibition after 72 hrs by MTT assay, IC50 = 29.6 μM. |
27101893 |
PC3 |
Cytotoxicity assay |
|
24 hrs |
Cytotoxicity against human PC3 cells after 24 hrs by MTT assay, IC50 = 30.3 μM. |
23802716 |
Saos2 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity against p53 deficient human Saos2 cells after 72 hrs by MTT assay, IC50 = 31.62 μM. |
23046248 |
HCT116 |
Antiproliferative assay |
|
48 hrs |
Antiproliferative activity against human HCT116 cells expressing wild type p53 after 48 hrs by MTT assay, GI50 = 32.11 μM. |
24852275 |
HCT116 |
Cytotoxicity assay |
|
72 hrs |
Cytotoxicity against p53 deficient human HCT116 cells after 72 hrs by sulforhodamine B assay, GI50 = 34 μM. |
21314128 |
HCT116 |
Antiproliferative assay |
|
24 hrs |
Antiproliferative activity against human HCT116 cells expressing p53 after 24 hrs by MTS assay, GI50 = 39.65 μM. |
24268795 |
A549 |
Antiproliferative assay |
|
48 hrs |
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay, GI50 = 47.59 μM. |
24852275 |
HCT116 |
Antiproliferative assay |
|
72 hrs |
Antiproliferative activity in p53 (-/-) human HCT116 cells incubated for 72 hrs by MTS assay, IC50 = 47.8 μM. |
28987608 |
MCF7 |
Antiproliferative assay |
|
48 hrs |
Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay, GI50 = 48.74 μM. |
24852275 |
MCF7 |
Function assay |
|
18 hrs |
Induction of p53 accumulation in human MCF7 cells after 18 hrs by Western blot analysis in presence of 2.5 mM caffeine |
16474381 |
MCF7 |
Function assay |
|
24 hrs |
Upregulation of p53 in human MCF7 cells after 24 hrs by Western blot analysis |
27010502 |
MCF7 |
Function assay |
|
24 hrs |
Upregulation of Mdm2 in human MCF7 cells after 24 hrs by Western blot analysis |
27010502 |
MCF7 |
Function assay |
|
24 hrs |
Increase in p53 expression in human MCF7 cells nuclei after 24 hrs by DAPI staining based immunofluorescence microscopy |
27010502 |
MCF7 |
Function assay |
|
24 hrs |
Upregulation of p21 in human MCF7 cells after 24 hrs by Western blot analysis |
27010502 |
IMR32 |
Function assay |
|
24 hrs |
Increase in p53 expression in human IMR32 cells nuclei after 24 hrs by DAPI staining based immunofluorescence microscopy |
27010502 |
697 |
Growth Inhibition Assay |
|
|
IC50=5.4455 μM |
SANGER |
MCF7 |
Growth Inhibition Assay |
|
|
IC50=5.44244 μM |
SANGER |
NCI-H460 |
Growth Inhibition Assay |
|
|
IC50=4.91127 μM |
SANGER |
D-247MG |
Growth Inhibition Assay |
|
|
IC50=4.75072 μM |
SANGER |
NCI-H28 |
Growth Inhibition Assay |
|
|
IC50=4.62717 μM |
SANGER |
CAL-54 |
Growth Inhibition Assay |
|
|
IC50=4.53019 μM |
SANGER |
MZ2-MEL |
Growth Inhibition Assay |
|
|
IC50=4.50155 μM |
SANGER |
IST-MES1 |
Growth Inhibition Assay |
|
|
IC50=4.41411 μM |
SANGER |
A375 |
Growth Inhibition Assay |
|
|
IC50=4.33524 μM |
SANGER |
NB17 |
Growth Inhibition Assay |
|
|
IC50=4.31768 μM |
SANGER |
RH-18 |
Growth Inhibition Assay |
|
|
IC50=4.27706 μM |
SANGER |
CP50-MEL-B |
Growth Inhibition Assay |
|
|
IC50=4.24392 μM |
SANGER |
CTV-1 |
Growth Inhibition Assay |
|
|
IC50=4.07973 μM |
SANGER |
NH-12 |
Growth Inhibition Assay |
|
|
IC50=4.01959 μM |
SANGER |
LOXIMVI |
Growth Inhibition Assay |
|
|
IC50=3.85675 μM |
SANGER |
GOTO |
Growth Inhibition Assay |
|
|
IC50=3.84333 μM |
SANGER |
G-402 |
Growth Inhibition Assay |
|
|
IC50=3.80832 μM |
SANGER |
SK-HEP-1 |
Growth Inhibition Assay |
|
|
IC50=3.74297 μM |
SANGER |
LB2241-RCC |
Growth Inhibition Assay |
|
|
IC50=3.65708 μM |
SANGER |
A549 |
Growth Inhibition Assay |
|
|
IC50=3.63552 μM |
SANGER |
LB996-RCC |
Growth Inhibition Assay |
|
|
IC50=3.62558 μM |
SANGER |
D-283MED |
Growth Inhibition Assay |
|
|
IC50=3.57986 μM |
SANGER |
RKO |
Growth Inhibition Assay |
|
|
IC50=3.53936 μM |
SANGER |
SW982 |
Growth Inhibition Assay |
|
|
IC50=3.50848 μM |
SANGER |
MN-60 |
Growth Inhibition Assay |
|
|
IC50=3.44092 μM |
SANGER |
G-401 |
Growth Inhibition Assay |
|
|
IC50=3.36322 μM |
SANGER |
HCT-116 |
Growth Inhibition Assay |
|
|
IC50=3.30335 μM |
SANGER |
NCI-SNU-1 |
Growth Inhibition Assay |
|
|
IC50=3.27843 μM |
SANGER |
MHH-NB-11 |
Growth Inhibition Assay |
|
|
IC50=3.26827 μM |
SANGER |
NB10 |
Growth Inhibition Assay |
|
|
IC50=3.20966 μM |
SANGER |
C2BBe1 |
Growth Inhibition Assay |
|
|
IC50=3.17026 μM |
SANGER |
CAKI-1 |
Growth Inhibition Assay |
|
|
IC50=3.12694 μM |
SANGER |
NCI-H292 |
Growth Inhibition Assay |
|
|
IC50=3.12028 μM |
SANGER |
KGN |
Growth Inhibition Assay |
|
|
IC50=3.08496 μM |
SANGER |
A204 |
Growth Inhibition Assay |
|
|
IC50=3.05588 μM |
SANGER |
KS-1 |
Growth Inhibition Assay |
|
|
IC50=3.01623 μM |
SANGER |
D-502MG |
Growth Inhibition Assay |
|
|
IC50=2.97154 μM |
SANGER |
CHP-134 |
Growth Inhibition Assay |
|
|
IC50=2.93882 μM |
SANGER |
U-2-OS |
Growth Inhibition Assay |
|
|
IC50=2.9107 μM |
SANGER |
BV-173 |
Growth Inhibition Assay |
|
|
IC50=2.84439 μM |
SANGER |
SJSA-1 |
Growth Inhibition Assay |
|
|
IC50=2.82556 μM |
SANGER |
DU-4475 |
Growth Inhibition Assay |
|
|
IC50=2.79502 μM |
SANGER |
RS4-11 |
Growth Inhibition Assay |
|
|
IC50=2.72407 μM |
SANGER |
A172 |
Growth Inhibition Assay |
|
|
IC50=2.67588 μM |
SANGER |
NB69 |
Growth Inhibition Assay |
|
|
IC50=2.65334 μM |
SANGER |
HT-144 |
Growth Inhibition Assay |
|
|
IC50=2.64577 μM |
SANGER |
LNCaP-Clone-FGC |
Growth Inhibition Assay |
|
|
IC50=2.64318 μM |
SANGER |
DK-MG |
Growth Inhibition Assay |
|
|
IC50=2.62471 μM |
SANGER |
A3-KAW |
Growth Inhibition Assay |
|
|
IC50=2.61818 μM |
SANGER |
JVM-3 |
Growth Inhibition Assay |
|
|
IC50=2.59324 μM |
SANGER |
MSTO-211H |
Growth Inhibition Assay |
|
|
IC50=2.57451 μM |
SANGER |
ML-2 |
Growth Inhibition Assay |
|
|
IC50=2.56576 μM |
SANGER |
RPMI-8866 |
Growth Inhibition Assay |
|
|
IC50=2.56214 μM |
SANGER |
VA-ES-BJ |
Growth Inhibition Assay |
|
|
IC50=2.5411 μM |
SANGER |
SW780 |
Growth Inhibition Assay |
|
|
IC50=2.50883 μM |
SANGER |
ES4 |
Growth Inhibition Assay |
|
|
IC50=2.43155 μM |
SANGER |
DOHH-2 |
Growth Inhibition Assay |
|
|
IC50=2.42279 μM |
SANGER |
A101D |
Growth Inhibition Assay |
|
|
IC50=2.3501 μM |
SANGER |
MOLT-4 |
Growth Inhibition Assay |
|
|
IC50=2.32853 μM |
SANGER |
CTB-1 |
Growth Inhibition Assay |
|
|
IC50=2.02246 μM |
SANGER |
CHP-212 |
Growth Inhibition Assay |
|
|
IC50=1.81369 μM |
SANGER |
MOLT-16 |
Growth Inhibition Assay |
|
|
IC50=1.78604 μM |
SANGER |
QIMR-WIL |
Growth Inhibition Assay |
|
|
IC50=1.60854 μM |
SANGER |
K5 |
Growth Inhibition Assay |
|
|
IC50=1.42072 μM |
SANGER |
EoL-1-cell |
Growth Inhibition Assay |
|
|
IC50=1.26701 μM |
SANGER |
NEC8 |
Growth Inhibition Assay |
|
|
IC50=1.21572 μM |
SANGER |
NKM-1 |
Growth Inhibition Assay |
|
|
IC50=1.04931 μM |
SANGER |
PA-1 |
Growth Inhibition Assay |
|
|
IC50=0.87096 μM |
SANGER |
H4 |
Growth Inhibition Assay |
|
|
IC50=0.6628 μM |
SANGER |
MV-4-11 |
Growth Inhibition Assay |
|
|
IC50=0.61237 μM |
SANGER |
ONS-76 |
Growth Inhibition Assay |
|
|
IC50=5.57009 μM |
SANGER |
C32 |
Growth Inhibition Assay |
|
|
IC50=5.60029 μM |
SANGER |
OS-RC-2 |
Growth Inhibition Assay |
|
|
IC50=5.73887 μM |
SANGER |
MEL-HO |
Growth Inhibition Assay |
|
|
IC50=5.85667 μM |
SANGER |
LoVo |
Growth Inhibition Assay |
|
|
IC50=6.01624 μM |
SANGER |
AGS |
Growth Inhibition Assay |
|
|
IC50=6.14828 μM |
SANGER |
GI-ME-N |
Growth Inhibition Assay |
|
|
IC50=6.22421 μM |
SANGER |
H-EMC-SS |
Growth Inhibition Assay |
|
|
IC50=6.386 μM |
SANGER |
RVH-421 |
Growth Inhibition Assay |
|
|
IC50=6.42428 μM |
SANGER |
SW954 |
Growth Inhibition Assay |
|
|
IC50=6.55572 μM |
SANGER |
NB5 |
Growth Inhibition Assay |
|
|
IC50=6.56183 μM |
SANGER |
NCI-H2122 |
Growth Inhibition Assay |
|
|
IC50=6.58793 μM |
SANGER |
AM-38 |
Growth Inhibition Assay |
|
|
IC50=6.75639 μM |
SANGER |
KNS-81-FD |
Growth Inhibition Assay |
|
|
IC50=6.76494 μM |
SANGER |
LS-513 |
Growth Inhibition Assay |
|
|
IC50=6.79026 μM |
SANGER |
A427 |
Growth Inhibition Assay |
|
|
IC50=6.87829 μM |
SANGER |
WM-115 |
Growth Inhibition Assay |
|
|
IC50=6.9323 μM |
SANGER |
COLO-829 |
Growth Inhibition Assay |
|
|
IC50=7.24188 μM |
SANGER |
NCI-H1650 |
Growth Inhibition Assay |
|
|
IC50=7.39228 μM |
SANGER |
NCI-H358 |
Growth Inhibition Assay |
|
|
IC50=7.44879 μM |
SANGER |
HT-1080 |
Growth Inhibition Assay |
|
|
IC50=7.48254 μM |
SANGER |
HCC2218 |
Growth Inhibition Assay |
|
|
IC50=7.6297 μM |
SANGER |
NCI-H661 |
Growth Inhibition Assay |
|
|
IC50=7.87069 μM |
SANGER |
KM-H2 |
Growth Inhibition Assay |
|
|
IC50=7.88694 μM |
SANGER |
RPMI-2650 |
Growth Inhibition Assay |
|
|
IC50=7.94414 μM |
SANGER |
NCI-H226 |
Growth Inhibition Assay |
|
|
IC50=8.21122 μM |
SANGER |
MKN45 |
Growth Inhibition Assay |
|
|
IC50=8.26602 μM |
SANGER |
D-392MG |
Growth Inhibition Assay |
|
|
IC50=8.52722 μM |
SANGER |
RCC10RGB |
Growth Inhibition Assay |
|
|
IC50=8.86695 μM |
SANGER |
CAL-51 |
Growth Inhibition Assay |
|
|
IC50=9.10251 μM |
SANGER |
COLO-678 |
Growth Inhibition Assay |
|
|
IC50=9.32811 μM |
SANGER |
SK-MEL-24 |
Growth Inhibition Assay |
|
|
IC50=9.55856 μM |
SANGER |
SK-MEL-30 |
Growth Inhibition Assay |
|
|
IC50=9.94476 μM |
SANGER |
MMAC-SF |
Growth Inhibition Assay |
|
|
IC50=10.3961 μM |
SANGER |
NTERA-S-cl-D1 |
Growth Inhibition Assay |
|
|
IC50=10.6508 μM |
SANGER |
NB12 |
Growth Inhibition Assay |
|
|
IC50=11.503 μM |
SANGER |
UACC-257 |
Growth Inhibition Assay |
|
|
IC50=11.8695 μM |
SANGER |
LAN-6 |
Growth Inhibition Assay |
|
|
IC50=11.9928 μM |
SANGER |
SW1573 |
Growth Inhibition Assay |
|
|
IC50=12.3086 μM |
SANGER |
NMC-G1 |
Growth Inhibition Assay |
|
|
IC50=12.4175 μM |
SANGER |
SHP-77 |
Growth Inhibition Assay |
|
|
IC50=12.5574 μM |
SANGER |
IGROV-1 |
Growth Inhibition Assay |
|
|
IC50=12.6573 μM |
SANGER |
22RV1 |
Growth Inhibition Assay |
|
|
IC50=12.8059 μM |
SANGER |
SK-MEL-3 |
Growth Inhibition Assay |
|
|
IC50=13.3973 μM |
SANGER |
NCI-H1563 |
Growth Inhibition Assay |
|
|
IC50=13.4202 μM |
SANGER |
IGR-1 |
Growth Inhibition Assay |
|
|
IC50=14.0347 μM |
SANGER |
EW-3 |
Growth Inhibition Assay |
|
|
IC50=14.1639 μM |
SANGER |
JEG-3 |
Growth Inhibition Assay |
|
|
IC50=14.4953 μM |
SANGER |
ES3 |
Growth Inhibition Assay |
|
|
IC50=14.6562 μM |
SANGER |
MDA-MB-175-VII |
Growth Inhibition Assay |
|
|
IC50=14.7111 μM |
SANGER |
P30-OHK |
Growth Inhibition Assay |
|
|
IC50=15.0496 μM |
SANGER |
GP5d |
Growth Inhibition Assay |
|
|
IC50=15.1466 μM |
SANGER |
HMV-II |
Growth Inhibition Assay |
|
|
IC50=15.3333 μM |
SANGER |
COLO-679 |
Growth Inhibition Assay |
|
|
IC50=15.501 μM |
SANGER |
JAR |
Growth Inhibition Assay |
|
|
IC50=15.7205 μM |
SANGER |
NCI-H1666 |
Growth Inhibition Assay |
|
|
IC50=15.9399 μM |
SANGER |
SW48 |
Growth Inhibition Assay |
|
|
IC50=15.9516 μM |
SANGER |
NCI-H720 |
Growth Inhibition Assay |
|
|
IC50=15.9989 μM |
SANGER |
HT-1197 |
Growth Inhibition Assay |
|
|
IC50=16.0352 μM |
SANGER |
HL-60 |
Growth Inhibition Assay |
|
|
IC50=16.0384 μM |
SANGER |
BEN |
Growth Inhibition Assay |
|
|
IC50=16.5638 μM |
SANGER |
HAL-01 |
Growth Inhibition Assay |
|
|
IC50=16.8909 μM |
SANGER |
SW900 |
Growth Inhibition Assay |
|
|
IC50=16.9132 μM |
SANGER |
SBC-1 |
Growth Inhibition Assay |
|
|
IC50=17.5429 μM |
SANGER |
SH-4 |
Growth Inhibition Assay |
|
|
IC50=17.5841 μM |
SANGER |
UACC-62 |
Growth Inhibition Assay |
|
|
IC50=17.7637 μM |
SANGER |
BHT-101 |
Growth Inhibition Assay |
|
|
IC50=19.8705 μM |
SANGER |
DB |
Growth Inhibition Assay |
|
|
IC50=21.5649 μM |
SANGER |
SK-MEL-1 |
Growth Inhibition Assay |
|
|
IC50=21.5872 μM |
SANGER |
NCI-H747 |
Growth Inhibition Assay |
|
|
IC50=22.7379 μM |
SANGER |
SK-LU-1 |
Growth Inhibition Assay |
|
|
IC50=23.3524 μM |
SANGER |
KG-1 |
Growth Inhibition Assay |
|
|
IC50=23.4946 μM |
SANGER |
LXF-289 |
Growth Inhibition Assay |
|
|
IC50=23.714 μM |
SANGER |
HCC1954 |
Growth Inhibition Assay |
|
|
IC50=24.8844 μM |
SANGER |
Ramos-2G6-4C10 |
Growth Inhibition Assay |
|
|
IC50=26.4981 μM |
SANGER |
DBTRG-05MG |
Growth Inhibition Assay |
|
|
IC50=26.6488 μM |
SANGER |
NCI-H2052 |
Growth Inhibition Assay |
|
|
IC50=27.5684 μM |
SANGER |
RMG-I |
Growth Inhibition Assay |
|
|
IC50=29.4139 μM |
SANGER |
H9 |
Growth Inhibition Assay |
|
|
IC50=31.3008 μM |
SANGER |
GR-ST |
Growth Inhibition Assay |
|
|
IC50=32.4453 μM |
SANGER |
Mo-T |
Growth Inhibition Assay |
|
|
IC50=32.5046 μM |
SANGER |
SW1088 |
Growth Inhibition Assay |
|
|
IC50=32.9801 μM |
SANGER |
LB2518-MEL |
Growth Inhibition Assay |
|
|
IC50=33.0797 μM |
SANGER |
NCI-H82 |
Growth Inhibition Assay |
|
|
IC50=33.1661 μM |
SANGER |
LAMA-84 |
Growth Inhibition Assay |
|
|
IC50=34.6073 μM |
SANGER |
KYSE-450 |
Growth Inhibition Assay |
|
|
IC50=34.6776 μM |
SANGER |
LU-99A |
Growth Inhibition Assay |
|
|
IC50=35.0007 μM |
SANGER |
BE-13 |
Growth Inhibition Assay |
|
|
IC50=35.6545 μM |
SANGER |
GAK |
Growth Inhibition Assay |
|
|
IC50=35.695 μM |
SANGER |
NCI-H1573 |
Growth Inhibition Assay |
|
|
IC50=35.8818 μM |
SANGER |
AsPC-1 |
Growth Inhibition Assay |
|
|
IC50=36.1527 μM |
SANGER |
HDLM-2 |
Growth Inhibition Assay |
|
|
IC50=36.316 μM |
SANGER |
NCI-H441 |
Growth Inhibition Assay |
|
|
IC50=37.0691 μM |
SANGER |
CAL-27 |
Growth Inhibition Assay |
|
|
IC50=37.7231 μM |
SANGER |
OVCAR-3 |
Growth Inhibition Assay |
|
|
IC50=39.3181 μM |
SANGER |
RPMI-8226 |
Growth Inhibition Assay |
|
|
IC50=39.598 μM |
SANGER |
EFO-21 |
Growth Inhibition Assay |
|
|
IC50=40.5814 μM |
SANGER |
SNU-C2B |
Growth Inhibition Assay |
|
|
IC50=41.5084 μM |
SANGER |
VM-CUB-1 |
Growth Inhibition Assay |
|
|
IC50=43.8037 μM |
SANGER |
NCI-H2087 |
Growth Inhibition Assay |
|
|
IC50=45.0233 μM |
SANGER |
EW-16 |
Growth Inhibition Assay |
|
|
IC50=46.3137 μM |
SANGER |
SK-N-AS |
Growth Inhibition Assay |
|
|
IC50=46.7167 μM |
SANGER |
COR-L105 |
Growth Inhibition Assay |
|
|
IC50=46.8857 μM |
SANGER |
DEL |
Growth Inhibition Assay |
|
|
IC50=48.0429 μM |
SANGER |
JVM-2 |
Growth Inhibition Assay |
|
|
IC50=48.0558 μM |
SANGER |
KARPAS-45 |
Growth Inhibition Assay |
|
|
IC50=49.4538 μM |
SANGER |
BL21 (DE3) |
Function assay |
|
|
Binding affinity to human recombinant Mdm2 T101W mutant expressed in Escherichia coli BL21 (DE3) cells by antagonist induced dissociation assay, Kd = 0.5 μM. |
18680271 |
BL21 (DE3) |
Function assay |
|
|
Binding affinity to human recombinant Mdm2 K98W mutant expressed in Escherichia coli BL21 (DE3) cells by antagonist induced dissociation assay, Kd = 0.5 μM. |
18680271 |
BL21 (DE3) |
Function assay |
|
|
Binding affinity to human recombinant Mdm2 F55W mutant expressed in Escherichia coli BL21 (DE3) cells by NMR ligand-protein binary titration, Kd = 0.5 μM. |
18680271 |
BL21 (DE3) |
Function assay |
|
|
Binding affinity to human recombinant Mdm2 T101W mutant expressed in Escherichia coli BL21 (DE3) cells by NMR ligand-protein binary titration, Kd = 1 μM. |
18680271 |
BL21 (DE3) |
Function assay |
|
|
Binding affinity to human recombinant Mdm2 K98W mutant expressed in Escherichia coli BL21 (DE3) cells by NMR ligand-protein binary titration, Kd = 1 μM. |
18680271 |
SJSA1 |
Cytotoxicity assay |
|
|
Cytotoxicity against human SJSA1 cells, IC50 = 1 μM. |
25396320 |
RKO |
Cytotoxicity assay |
|
|
Cytotoxicity against human RKO cells, IC50 = 1 μM. |
25396320 |
HCT116 |
Cytotoxicity assay |
|
|
Cytotoxicity against human HCT116 cells, IC50 = 1 μM. |
25396320 |
SJSA1 |
Growth inhibition assay |
|
|
Growth inhibition of human SJSA1 cells expressing MDM2 by SRB assay, GI50 = 1.3 μM. |
21875801 |
A2780 |
Growth inhibition assay |
|
|
Growth inhibition of human A2780 cells expressing MDM2 by SRB assay, GI50 = 1.6 μM. |
21875801 |
SJSA1 |
Antiproliferative assay |
|
|
Antiproliferative activity against human SJSA1 cells, IC50 = 1.9 μM. |
24656661 |
HCT116 |
Growth inhibition assay |
|
|
Growth inhibition of human HCT116 cells expressing MDM2 by SRB assay, GI50 = 2.1 μM. |
21875801 |
A2780/CP70 |
Growth inhibition assay |
|
|
Growth inhibition of human A2780/CP70 cells expressing isogenically paired p53 mutant by SRB assay, GI50 = 20.4 μM. |
21875801 |
HCT116 |
Growth inhibition assay |
|
|
Growth inhibition of p53 deficient human HCT116 cells expressing MDM2 by SRB assay, GI50 = 25.6 μM. |
21875801 |
MCF7 |
Function assay |
|
|
Induction of p53 expression in human MCF7 cells transfected with 53BP1-targeting iRNA-based shRNA by Western blot analysis |
16474381 |
MCF7 |
Function assay |
|
|
Induction of p53 expression in human MCF7 cells by Western blot analysis |
16474381 |