Z-VAD-FMK

別名：Z-VAD(OMe)-FMK

製品コード：S7023 純度：99.79%

Z-VAD-FMK (Z-VAD(OMe)-FMK) は、細胞透過性で不可逆的な汎カスパーゼ阻害剤であり、THP.1 および Jurkat T 細胞のアポトーシス (apoptosis) をブロックします。

CAS No. 187389-52-2

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 59500	国内在庫なし(納期7~10日)
1mg	JPY 26500	国内在庫あり
5mg	JPY 52300	国内在庫あり
25mg	JPY 145500	国内在庫あり
1g	JPY 895500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（1013）

製品安全説明書

現在のバッチを見る：純度： 99.79%

99.79

Z-VAD-FMKと併用されることが多い化合物

Necrostatin-1

Z-VAD-FMK and Necrostatin-1 protect NP cells from compression-induced death and restore mitochondrial function.

Chen S, et al. Biomed Res Int. 2020 Dec 8;2020:6976317.

Fer-1 (Ferrostatin-1)

Z-VAD-FMK and Ferrostatin-1 treatment attenuates the loss of synaptic proteins and restore cognitive function in both hippocampal-dependent and hippocampal-independent manners in iron-overloaded rats.

Sripetchwandee J, et al. Life Sci. 2023 Jan 15;313:121269.

Y-27632 2HCl

Z-VAD-FMK and Y-27632 2HCl block membrane blebbing and nucleus condensation induced by H2O2 in PC12 cells.

Wang Y, et al. Front Mol Neurosci. 2017 Mar 14;10:75.

3-MA (3-Methyladenine)

Z-VAD-FMK diminishes dehydrocostus lactone (DHL)-induced autophagy, whereas 3-methyladenine (3-MA) has no effect on dehydrocostus lactone (DHL)-induced apoptosis.

Peng Y, et al. Food Chem Toxicol. 2022 Dec;170:113453.

Erastin

Erastin treatment in combination with Z-VAD-FMK does not block erastin-induced cell death in HS578T/MDAMB231 cells.

Che C, et al. Cell Death Dis. 2022 Feb 14;13(2):150.

Z-VAD-FMK関連製品

関連ターゲット	Caspase-1 Caspase-3 Caspase-8 Caspase-9 Caspase-4 Capase-7 Caspase-2 Caspase-5 Caspase-6 Caspase-10 Caspase-7	もっと見る
関連製品	Belnacasan (VX-765) Z-DEVD-FMK Q-VD-Oph Z-IETD-FMK Emricasan (IDN-6556) Ac-DEVD-CHO Z-LEHD-FMK TFA Z-VAD(OH)-FMK (Caspase Inhibitor VI) PAC-1 Z-YVAD-FMK Apoptosis Activator 2 Tasisulam Phenoxodiol (Haginin E)	もっと見る
関連化合物ライブラリー	Autophagy Compound Library Apoptosis Compound Library Ferroptosis Compound Library Pyroptosis Compound Library Mitochondria-Targeted Compound Library	もっと見る

シグナル伝達経路

Caspaseシグナル伝達経路

Caspase阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
LNCaP	Apoptosis Assay	40μM	2h	inhibits butein induced cell apoptosis	22114764
MDA-MB-231	Apoptosis Assay	2.5-7.5μM	2h	inhibits the cell death of MDA-MB-231 cells induced by SDT in a concentration dependent manner	22115526
Neutrophil	Apoptosis Assay	20μM	0.5h	attenuates the pro-apoptotic effect of MaR1	26196844
Jurkat T	Apoptosis Assay	30μM	0.5h	blocks the ziram-induced apoptosis	22159898
HeLa	Apoptosis Assay	10μM	0.5h	inhibits apoptosis induced by the combined treatment with gomisin N and TRAIL	22179661
Molt4-hyg	Apoptosis Assay	10μM	0.5h	blocks farnesol-induced caspase-3-like activity	26275811
K562	Apoptosis Assay	20 μM	48h	blocks inhibition of viability and apoptosis induction	22216158
EMT-6	Cytotoxicity Assay	100μM	1h	partially blocked cell death induced by siramesine	22251921
HCC	Apoptosis Assay	20 μM	2h	attenuates the DHA induced activation of PARP	22342732
T47D	Apoptosis Assay	100μM	1h	blocks the generation of E-cad/CTF2 by STS	22401168
HeLa	Apoptosis Assay	50 μM	1h	suppresses the FRAP-induced accumulation of annexin V positive cells	22449440
HeLa	Apoptosis Assay	50 μM	4/8h	inhibits STS-induced late-phase apoptotic events	22460504
SMMC-7721	Apoptosis Assay	50μM	48h	attenuates oTR-induced apoptosis	22465833
PC3	Apoptosis Assay	10μM	4h	counters flavocoxid-induced caspase-related apoptosis	22471974
HL-60	Apoptosis Assay	100μM	24h	inhibits the cell apoptosis selected (+)-menthyl β-(1→6)-diglucopyranoside 5	22546669
MDA-MB-231	Apoptosis Assay	20μM	4d	reduces the cell apoptosis induced by treatment was significantly	22593441
3T9 MEFs	Function Assay		18h	upregulates initiator caspase-9 but downregulates effector caspases after treatment	22613767
shC9	Apoptosis Assay	10μM	16h	blunts SHH expression in shC9 cells exposed to either PA or LPC	22641094
AGS	Apoptosis Assay	20μM	12h	inhibits the activation of pro-caspase-3 in response to the EtOAc fraction	22687398
A549	Apoptosis Assay	50μM	1h	prevents apoptosis induced by Baohuoside I	22687635
PMNs	Apoptosis Assay	40μM	6h	reversed the amount of cleaved caspase-3 to near vehicle levels	22692577
Primary OPC	Apoptosis Assay	1μM	6h/24h	reduces the percentage of cells in early- and late-apoptosis/necrosis	22707385
A549	Apoptosis Assay	50μM	2h	partially decreases sodium selenite-induced apoptosis	22721804
HT1080	Growth Inhibition Assay	50μM	7d	inhibits the cell growth inhibition caused by combined treatment of DCA and OMP	22740984
U87	Apoptosis Assay	20μM	24h	reduces the apoptosis rate induced by PLAB	22778780
A2750	Apoptosis Assay	20μM	2h	blocks caspase cleavage during helenalin treatment and reduces autophagic cell death	22784363
DM6	Apoptosis Assay	100μM	72h	blocks both E2F-1 and E2Ftr-mediated cytotoxicity	22825328
A549	Apoptosis Assay	50μM	1h	blocks the BAI-induced apoptosis	22887215
BGC-823	Apoptosis Assay	100μM	1h	partially rescues cells against damage of daidzein	22926545
Jurkat	Apoptosis Assay	40μM	1h	abolishes FasL-induced caspase activation and cell death	22942738
K562	Apoptosis Assay	100μM	1h	blocks Abnobaviscum F-induced apoptosis	22972372
NB2a/d1	Apoptosis Assay	100μM	72h	attenuates staurosporine-induced caspase activity, PARP, and tau cleavage	22988541
RCC	Cell Viability Assay	100μM	24h	recoveres the viability of cells exposed to 15d-PGJ2	22991494
L929	Function Assay	2.5μM	1h	increases RIP1 expression and exacerbated TNFα-induced mitochondrial dysfunction and ROS production	23000518
Bel-7402	Apoptosis Assay	50μM	2h	inhibits PCE-induced anoikis	23008742
MEL	Apoptosis Assay	20μM	1h	impaires P2X7-induced MEL cell apoptosis	23014887
Eca-109	Apoptosis Assay	25μM	30min	inhibits BJ-B11-induced cell death	23076967
Bel-7402	Caspase Activation Assay	50μM	2h	inhibits PCE-induced anoikis	23008742
MEL	Apoptosis Assay	20μM	24h	impaires P2X7-induced MEL cell apoptosis	23014887
CL-1	Apoptosis Assay	20μM	1h	blocks OP449 induced cell death	23131782
HSC-4	Apoptosis Assay	25/50μM	1h	inhibits SN-38-induced cytotoxicity	23155248
HSC-2	Apoptosis Assay	25/50μM	1h	inhibits SN-38-induced cytotoxicity	23155248
HepG2	Apoptosis Assay	20μM	30min	inhibits the enhanced cell death by combined treatment of apigenin and TRAIL	23224239
QGY7701	Apoptosis Assay	25μM	1.5h	inhibits accumulation of sub-G1 phase induced by DOX + quercetin	23240061
SK-HEP1	Apoptosis Assay	100μM	1h	inhibits CrT1 activated caspase-3, -7, -8, -9, and poly(ADP-ribose) polymerase	23302650
HeLa	Apoptosis Assay	50μM	1.5h	abrogates Chlamydia-induced apoptosis	23303804
H460	Apoptosis Assay	10μM	6h	blocks apoptotic combination of Tat-SmacN7 and radiation	23338568
Ec109	Apoptosis Assay	10μM	6h	blocks apoptotic combination of Tat-SmacN7 and radiation	23338568
HeLa	Apoptosis Assay	100μM	2h	blocks JRS-15 Induces Apoptotic Cell Death	23344045
MDA-MB-231	Apoptosis Assay	100μM	1h	inhibits sensitization to TRAIL upon MTDH down-regulation	23408429
U-937	Apoptosis Assay	10μM	30min	prevents TNF-induced necroptosis	23410748
astrocytes cell	Apoptosis Assay	40μM	6h	reduces early apoptosis induced by staurosporine	23411778
HONE-1	Apoptosis Assay	20μM	24h	inhibits RAD001-induced cell death	23426850
CNE-1	Apoptosis Assay	20μM	24h	inhibits RAD001-induced cell death	23426850
Jurkat	Apoptosis Assay	12.5-50μM	1h	dose dependently suppresses SPH-induced Par-4 cleavage, PARP cleavage, DNA fragmentation, and loss of viability	23442976
hCMEC/D3	Apoptosis Assay	25μM	72h	reduces LtxA induced apoptosis	23665198
MCF-7	Apoptosis Assay	20μM	72h	inhibits equol- and 4-OHT-mediated apoptosis	23675643
KNS42	Apoptosis Assay	50μM	1h	reduces cell death and completely abolished caspase 3/7 activity in response to ABT-263/2DG/ combination	23691145
NLRP3-Tet-on-MC/9	Apoptosis Assay	10-40μM	12h	attenuates the cell death caused by CAPS-associated NLRP3 mutants in the Tet-on system	23703389
MDA-MB-231	Apoptosis Assay	25μM	24h	abrogates cytotoxicity and cleavage of caspase-3 and PARP induced by Rg3	25337544
HSCs	Apoptosis Assay	50μM	24h	inhibits nilotinib-induced DNA damage indicated by PARP cleavage	23499874
Hep-2	Apoptosis Assay	20μM	24h	alleviates the decrease of cell viability induced by silibinin	23580032
HM7	Apoptosis Assay	20μM	1h	blocks apicularen A acetate-induced caspase-3 activation and PARP cleavage	23583412
HL-60	Apoptosis Assay	50μM	48h	reduces cell death caused by SKI-II treatment	25824043
DTK-SME	Apoptosis Assay	50μM	2h	partially inhibits the apoptosis induced by bupivacaine	25843897
HCT116	Apoptosis Assay	50μM	2h	inhibits the cell death induced by DDVP	25868818
SGN	Apoptosis Assay	20mM	48h	has no influence on AIF, calpain expression or cell apoptosis	25874633
A549/V16	Apoptosis Assay	50μM	1h	represses TG/TM-induced apoptosis	25946033
U1	Kinase Assay	100μM	2h	inhibits caspase-3	25980942
ACH-2	Apoptosis Assay	0-200μM	2h	reduces drug-induced apoptosis and subsequent HIV-1 replication in a dose-dependent manner	25980942
BEL-7402	Apoptosis Assay	20μM	1h	attenuated the apoptotic induction of III-10	26164795
HepG2	Apoptosis Assay	20μM	1h	attenuated the apoptotic induction of III-10	26164795
SGC-7901	Apoptosis Assay	20 μM	1h	inhibits the cell death induced by oxaliplatin	25767076
MCF-7	Apoptosis Assay	10μM	2h	inhibits the cell death induced by 3B	25722114
A549	Apoptosis Assay	10 μM	24h	promotes HBC-treated A549 cell survival & attenuates the cleaved PARP expression	25683568
U87	Growth Inhibition Assay	50μM	1h	recovers cell growth from TMZ treatment	25681668
Neocortical Neuron	Neurotoxicity Assay	100μM	1h	antagonizes Hoiamide A-Induced Neurotoxicity	25675001
Primary human placental cytotrophoblasts	Apoptosis Assay	30μM	24h	reverses the inhibition of 11β-HSD2 by triclosan	25642592
MM	Apoptosis Assay	50μM	20min	partly inhibits SHK-induced cell death	25530098
DLD1	Apoptosis Assay	20 μM	1h	partly reverses cell apoptosis caused by WSP1	25524246
MDA-MB-231	Cytotoxicity Assay	10μM	1h	augments cell death after CHUA treatment	25521501
HL-60	Cytotoxicity Assay	100μM	1h	improves viability of TCNAs-treated cells	25502932
AGS	Apoptosis Assay	10μM	1h	prevents curcumin-induced cleavage of caspase-3, -8, and -9 proteins	25492214
A549	Apoptosis Assay	10μM	24h	blocks DMAS-induced cleavage of caspase-3 and PARP and apoptotic cell death	25434989
INS-1	Apoptosis Assay	50μM	6h	decreases the proportion of early apoptotic cells	25430897
L929-A	Cytotoxicity Assay	20 μM	24h	inhibits TNFα-induced cell death	25398540
L929-N	Cytotoxicity Assay	20 μM	24h	enhances death via autocrine TNFα production	25398540
A549	Apoptosis Assay	50μM	24h	reverses ribosome biogenesis and apoptosis caused by Chijongdan	25349781
A549	Apoptosis Assay	10μM	3h	reduces cell apoptosis caused by PQQ	25161699
MCF-7	Apoptosis Assay	20μM	2h	decreases KDR-siRNA-induced apoptosis	25182240
OS	Apoptosis Assay	20/40μM	72h	inhibits OS cell viability reduction by C6 ceramide	25152399
EA.hy926	Apoptosis Assay	10μM	2h	inhibits apoptosis and facilitates autophagy in DENV2-infected EA.hy926	25138703
Ebs	Apoptosis Assay	10-100μM	24h	MMTS generation rate decreased as the concentration of z-VAD.fmk increased	25134817
Jurkat	Apoptosis Assay	20μM	24h	partially inhibit cell death of Jurkat cells induced by 10058-F4 combined with VPA	25120723
K562	Apoptosis Assay	0.1-1μM	1h	inhibits cleavage of HSP90 in a dose-dependent manner	25119188
IMR-32	Apoptosis Assay	40 μM	2h	decrease in apoptotic cells compared to T-2 toxin	25084755
RPMI 8226	Apoptosis Assay	100μM	1h	almost completely blocks cell death caused by necrostatin-1	25037851
U266	Apoptosis Assay	100μM	1h	partially blocks cell death caused by necrostatin-1	25037851
H929	Apoptosis Assay	100μM	1h	partially blocks cell death caused by necrostatin-1	25037851
MM.1S	Apoptosis Assay	100μM	1h	efficiently prevents sorafenib-induced cell death combine with necrostatin-1	25037851
C6	Apoptosis Assay	50μM	48h	prevents the loss of cell viability caused by pregnenolone	25013479
AGS	Apoptosis Assay	50μM	24h	abolishs β-lapachone-induced cell death and inhibited growth	25009698
LOVO	Apoptosis Assay	10 µM	1h	restrains cell apoptosis induced by plus	24874286
macrophage	Cell Viability Assay	0-200μM	24h	induces TNF- and Rip3-dependent necroptosis in macrophage	24799565
U937	necroptosis Assay	10 μM	30 min	induces necroptosis combine with TNF	24773756
T cell	Cell proliferation assay	0-100μM	72h	IC50=70 μM, inhibits anti-CD3-induced T cell proliferation in PBMCs	24768707
HeLa	Apoptosis Assay	50μM	72h	prevents apoptosis induced by compounds 1–5	24754786
HeLa	Apoptosis Assay	100μM	24h	suppresses rate of cell death induced by overexpression of G59S or G71R p150glued	24722468
IEC-6	Apoptosis Assay	10μM	24h	prevents TcdA-induced caspase-3 cleavage and β-catenin degradation	24711571
HCT116	Apoptosis Assay	20μM	24h	inhibits the cell apoptotic induced by YLT205	24713812
L02	Apoptosis Assay	20μM	1h	prevents the cell apoptosis induced by MEHP	24706461
A375	Apoptosis Assay	20μM	24h	reverses dicitrinone B-induced apoptosis	24699111
HDPC	Apoptosis Assay	50µM	24h	inhibits NO-induced apoptosis	24634593
A549	Apoptosis Assay	50µM	2h	prevents the hypodiploid DNA content phase induced by cephalochromin	24588135
BV-2	Apoptosis Assay	20μM	2h	suppresses UV-induced chromatin extrusion and dilation of the nuclear envelope	24558118
HT-29	Apoptosis Assay	50μM	48h	blocks the apoptosis induced by kaempferol	24549175
A549	Apoptosis Assay	5μM	24h	suppresses 6e (BK10040)-induced apoptosis	24529871
MIA-PaCa-2	Apoptosis Assay		24h	blocks cleavage of caspase-3 induced by both A-443654 and paclitaxel	24510992
Caki-1	Apoptosis Assay	20μM	1h	inhibits SCP-induced apoptosis	24504681
CLL	Apoptosis Assay	25μM	1h	partially blocks MLN2238-induced cell apoptosis	24467634
k1735	Apoptosis Assay	20 μm	4h	inhibits Salmonella-induced apoptosis	24451116
HaCaT	Apoptosis Assay	100μM	1h	blunts UVB-induced apoptosis in HaCaT cells	24356997
podocytes	Apoptosis Assay	200μM	6h	inhibits apoptosis induced by 3,4-DGE	24337777
KB	Apoptosis Assay	50μM	24h	inhibits Lico-A-induced caspase-3 and PARP activation	24337492
HepG2	Apoptosis Assay	10μM	24h	reduces vimentin cleavage caused by LPS	24325816
fetal rat lung fibroblasts	Kinase Assay	80μM	1h	inhibits Caspase-3 activity	24314135
PDL fibroblasts	Kinase Assay	80μM	1h	inhibits Caspase-3 activity	24314135
MB49	Apoptosis Assay	20μM	1h	reverses CM-induced cell death	24282433
5637	Apoptosis Assay	20μM	1h	reverses CM-induced cell death	24282433
A549	Apoptosis Assay	100μM	48h	suppresses the apoptosis caused by piperine	24272201
MCC-2	Apoptosis Assay	20 μM	6h	blocks induced by staurosporine apoptosis cell death	24262658
ADF	Apoptosis Assay	40μM	6h	inhibits apoptosis induced by chemotherapy plus ZnCl2	24228232
U373	Apoptosis Assay	40μM	6h	inhibits apoptosis induced by chemotherapy plus ZnCl2	24228232
RKO-HIPK2i	Apoptosis Assay	40μM	6h	inhibits apoptosis induced by chemotherapy plus ZnCl2	24228232
4T1	Cell Viability Assay	2.5-10μM	4h	rescues the cytotoxicity of 4T1 cells induced by SPDT in a concentration dependent manner	24206161
OS	Apoptosis Assay	20μM	4h	inhibits the cell death caused by PDT	24204937
RPE	Apoptosis Assay	100μM	48h	partially inhibits the calpain-1 and -2 activation as well as the caspase activation	24202052
UD29a	Apoptosis Assay	50μM	24h	inhibits the cell death caused by NUT3	24190574
SH-SY5Y	Apoptosis Assay	10μM	24h	attenuates caspase activation and cell death induced by HNE-2DG	24145463
HeLa	Apoptosis Assay	10/20μM	24h	inhibits the activity of the majority of the members of the caspase-family	24137266
SCCVII	Apoptosis Assay	25μM	1h	inhibits the cell killing after	24126464
RAW 264.7	Apoptosis Assay	20μM	18h	increases LC3-II/β-actin ratio compared to ECTV-MOS treatment only	24116707
UM-SCC-10A	Apoptosis Assay	50µM	2h	reduces apoptosis induced by high-dose isoalantolactone	24098753
BGC-823	Apoptosis Assay	10 μM	24h	reduces tetrandrine-induced apoptosis	24098511
Nalm-6	Apoptosis Assay	20μM	4h	inhibits caspase-8 and caspase-3 activation and PARP-1 cleavage	24039967
KHOS	Apoptosis Assay	40μM	24h	blocks the increased cleavage of caspase-3 and PARP induced by BBMD3	24025361
G292	Apoptosis Assay	40μM	24h	blocks the increased cleavage of caspase-3 and PARP induced by BBMD3	24025361
MG-63	Apoptosis Assay	40μM	24h	blocks the increased cleavage of caspase-3 and PARP induced by BBMD3	24025361
CNE1	Apoptosis Assay	20μM	48h	blocks LK-A-induced apoptosis	23985029
CNE2	Apoptosis Assay	20μM	48h	blocks LK-A-induced apoptosis	23985029
L929	Apoptosis Assay	1.25–5 μM	24h	augments TNFα-induced necroptosis and autophagy	23941769
RAW264.7	Apoptosis Assay	10 μM	24h	decreases the apoptosis induced by GA	23820203
A431	Kinase Assay	40μM	2h	blocks fisetin-induced cleavage of caspases and PARP	23800058
Jurkat	Cell Viability Assay	25-100μM	6h	inhibits z-FA-CMK-induced cell death	23933532
COS-7	Function Assay	50µM	24h	affects the processing of ATN1s with polyQ tracts	23933208
HL-60	Apoptosis Assay	100μM	24h	inhibits apoptosis induced by abietane diterpenes	23865778
HL-60	Apoptosis Assay	50µM	4h	blocks the cleavage of caspase-3, -9, and PARP induced by 6s	23804706
Ec-109	Apoptosis Assay	10 μM	48h	represses PE-mediated Ec-109 cell apoptosis	23782641
RKO	Cell Viability Assay	10 μM	24h	inhibits the decrease of cell viability caused by DAB	23758064
T98G	Cell Viability Assay	1-100μM	24h	improves cell viability cotreatment with TS	23769275
Jurkat	Cell Viability Assay	100-200μM	24h	inhibits HaA4 induces apoptosis dependent of concentration	23732481
HCT116	Apoptosis Assay	50 μM	2h	reverses synergistic apoptosis effects of and NPC-16	22159752
MCF7	Apoptosis Assay	50 μM	1h	inhibits PA activated caspase-3, -9, and poly(ADP-ribose) polymerase	22223345
mESCs	Apoptosis Assay	2.5μM	2h	inhibits the NaF-mediated caspase activation	22285274
HeLa	Apoptosis Assay	30μM	4h	increases the general cell viability 48 h after photodynamic treatment	22394248
SGC-7901	Apoptosis Assay	20 μM	2h	attenuates H2O2 or TNF α-induced cell apoptosis as well as caspase-3 activity	22471589
K562	Apoptosis Assay	25μM	2h	prevents apoptosis induced by co-treatment with amurensin G and TRAIL	22483777
RAW 264.7	Apoptosis Assay	50/100μM	1h	concentration-dependent inhibits DON-induced rRNA cleavage	22491426
BCC	Apoptosis Assay	50μM	1h	inhibits DATS-mediated growth inhibition	22519436
HL-60	Apoptosis Assay	10-80μM	4h	inhibits TGHQ-induced cell apoptosis	22523229
C6	Apoptosis Assay	10μM	24h	blocks the suppressive effect of the peptide on viability	22588980
3T9 MEFs	Function Assay		16h	increases cytochrome c release after	22613767
primary MEFs	Function Assay		12h	increases mitochondrial membrane depolarization	22613767
MCF-7, MDA-MB-468, Caco-2	Growth Inhibition Assay	50μM	48h	inhibits the cell growth inhibition induced by saponin	22800968
SGC-7901	Apoptosis Assay	10μM	24h	inhibits β,β-dimethylacrylshikonin-induced apoptosis	22848597
LLC-PK1	Apoptosis Assay	20μM	1h	prevents degradation of Atg5, beclin-1, and Atg12 proteins	22896037
Hep3B	Apoptosis Assay	50μM	1h	blocks apoptosis induced by HEGCs	22923154
T cell	Growth Inhibition Assay	25-100μM	24h	dose-dependently inhibited T cell proliferation mediated through the co-stimulation with anti-CD3 and anti-CD28	22982538
U87	Apoptosis Assay	25μM	2h	decreases isoliquiritigenin (ISL)-induced apoptotic cell death, but not necrotic cell death	23229626
Primary rat cerebral cortical neurons	Apoptosis Assay	100μM	1h	prevents Cd-induced apoptosis and cell death	23741317
HCEC	Function Assay	50μM	72h	restores of the normal HCEC phenotype	23742011
HCT116	Apoptosis Assay	100μM	1.5h	inhibits Os-induced cell death	23744353
PLC/PRF/5c	Apoptosis Assay	50μM	1h	prevents apoptosis triggered by CIN	23438824
Hepa1-6	Apoptosis Assay	50μM	2h	inhibits curcumin and resveratrol-induced apoptosis	23446753
C666-1	Apoptosis Assay	50μM	24h	suppresses the cleavage of PARP and caspase-3, -7, and -9 induced by	23475956
HA	Apoptosis Assay	50μM	24h	suppresses the cleavage of PARP and caspase-3, -7, and -9 induced by	23475956
HL-60	Cytotoxicity Assay	20μM	24h	reduces the cytotoxic effect of Ery5	23494480
hMSC12	Apoptosis Assay	100μM	4d	inhibits osteogenic culture-induced cell death and calcification	23657822
U937	Apoptosis Assay	50μM	48h	reduces cell death caused by SKI-II treatment	25824043
U1	Apoptosis Assay	0-100μM	2h	reduces drug-induced apoptosis and subsequent HIV-1 replication in a dose-dependent manner	25980942
HUVEC-2c	Apoptosis Assay	50μM	6h	decreased the ox-LDL-induced autophagy	26021729
SP2/0	Apoptosis Assay	100μM	1h	blocks the apoptosis of SP2/0 cells	26074732
CEF	Kinase Assay	0,33,67,100μM	15min	down-regulates PR enzyme activity to 40％ at 100μM	26102339
HGL5	Apoptosis Assay	50μM	24h	indicates a protective effect against	26169075
GC1a	Apoptosis Assay	50μM	24h	indicates a protective effect against	26169075
A549	Apoptosis Assay	20 μM	24h	reduces cell death caused by HFCP treatment	25794149
HeLa	Apoptosis Assay	10μM	48h	declines the rate of apoptosis dramatically	25772545
MKN28	Apoptosis Assay	10μM	30min	inhibits TNF-α plus CHX-induced apoptosis	25513960
Primary hepatocytes	Apoptosis Assay	50μM	18h	inhibits apoptosis of hepatocytes induced by Act D and TNF-α	25407538
Cytotoxicity Assay	Cell Viability Assay	20μM	48h	prevent MHMD-induced cell death	25392116
COS7	Kinase Assay	10μM	24h	increases caspase 3/7 activities	25384025
COS7	Apoptosis Assay	10μM	48h	partially prevented FC101-induced cell death	25384025
A375	Apoptosis Assay	30μM	2h	prevents the drug-induced PARP cleavage	25376115
A549	Apoptosis Assay	2.5-25μM	1h	decreases the population of apoptotic cells depend on concentrations	25342427
NBL-W-S	Apoptosis Assay	50μM	1h	fully rescues cell viability after GANT-61 treatment	25323222
Caki-1	Apoptosis Assay	40μM	48h	reduces the number of Annexin V-positive cells	25279191
769-P	Apoptosis Assay	40μM	48h	reduces the number of Annexin V-positive cells	25279191
H9c2	Apoptosis Assay	50μM	1h	inhibits DOX-induced caspase 3 activation but not the loss of cells	25283819
K562	Apoptosis Assay	50μM	4h	inhibits Jac-A-induced cell apoptosis	25241619
L929	Apoptosis Assay	10μM	6h	induces necroptosis with TNF	25195660
HUVECs	Apoptosis Assay	10μM	2h	inhibits apoptosis and facilitates autophagy in DENV2-infected HUVECs	25138703
HeLa	Apoptosis Assay	40μM	24h	inhibits the increased apoptosis induced by siRNF121	24928685
THP-1	Apoptosis Assay	10μM	1h	reduces apoptosis induced by ALA-SDT	24923653
P815	Apoptosis Assay	100 µM	12h	inhibits virus-induced apoptosis	24923273
HCT116	Apoptosis Assay	20μM	24h	abrogates of TQ-induced apoptosi	24890449
SW1116	Apoptosis Assay	10 µM	1h	restrains cell apoptosis induced by plus	24874286
SNU449	Apoptosis Assay	20μM	48h	decreases miR-451-induced apoptotic	24841638
Caco-2	Apoptosis Assay	40 μM	4h	prevents ST-7-induced ZO-1 changes and TER drop	24822183
AGS	Apoptosis Assay	20μM	12h	reduces the induction of apoptosis in response to the EtOAc fraction in a dose-dependent manner	24789703
LCC9	Cell Viability Assay	100μM	5d	blocks cell death induced by combination L17 and	24785256
SGC-7901	Apoptosis Assay	10μM	24h	promotes the CGII-inhibited cell growth in SGC-7901 cells	24454488
HEC-1B	Apoptosis Assay	20μM	1h	reduces TP-induced apoptosis caspase-3 and caspase-9	24213358
A549	Apoptosis Assay	2.5μM	24h	decreases oridonin-induced autophagy as well and Loss of Δψm also occurred during autophagic process	24102522
EBV-transformed B cells	Apoptosis Assay	20μM	2h	blocks anti-CD80 and anti-CD86 antibody-induced apoptosis	24008628
IM-9 B	Apoptosis Assay	20μM	2h	blocks anti-CD80 and anti-CD86 antibody-induced apoptosis	24008628
HL-60	Apoptosis Assay	50µM	1h	inhibits apoptosis induced by BA145	23948751
Jurkat	Cell Viability Assay	2μM	4h	reverses growth inhibition and β-catenin decrease by HS-ASA	23896061
CAL27	Apoptosis Assay	10 μM	2h	suppresses Cur-NPs-reduced viability by up to 90%	23917396
THP-1	Apoptosis Assay	10-50μM	2h	inhibits apoptosis induced by triptolide	23900299
K562	Apoptosis Assay	20μM	48h	inhibits apoptosis induced by 4-MU	23876826
HeLa	Apoptosis Assay	20μM	2h	inhibits DMMP induced apoptosis	23863966
U251	Apoptosis Assay	20μM	24h	inhibits casticin induced G2/M phase arrest and apoptosis	23816816
Molt-3	Apoptosis Assay	50μM	2h	reduces melatonin-induced apoptosis	23725013
MB231	Apoptosis Assay	100μM	1h	abrogates the induction of cell death by WEE1 inhibition, TRAIL treatment, and the combination	22112940
HCC38	Apoptosis Assay	100μM	1h	abrogates the induction of cell death by WEE1 inhibition, TRAIL treatment, and the combination	22112940
MDA-MB231	Apoptosis Assay	50 μM	24h	abrogates curcumin-induced cell death	22101335
LNCaP	Apoptosis Assay	50 μM	24h	abrogates curcumin-induced cell death	22101335
HCT116	Apoptosis Assay	50 μM	24h	abrogates curcumin-induced cell death	22101335
Ishikawa	Apoptosis Assay	25μM	24h	reduces cell death and apoptosis induced by Baf A1	22088918
YD-8	Apoptosis Assay	100μM	1h	inhibits PLEO-induced apoptosis	22086183
eosinophil	Apoptosis Assay	90μM	24h	inhibits apoptosis induced by Fas antibody	22079334
L929	Growth Inhibition Assay	2.5μM	24h	inhibits the expression of p-p38 and NF-κB to augment TNFα-induced necroptosis and autophagy	22027097
YD-8	Apoptosis Assay	100μM	1h	blocks the GS-HCl-induced apoptosis	22020078
HBx	Apoptosis Assay	25μM	48h	reduced cell death induced by 3-MA	22020078
U937	Apoptosis Assay	50 μM	1h	inhibits HF-induced apoptosis	21998731
HL60	Apoptosis Assay	40μM	40min	blocks BNDC compounds induce exposure of phosphatidylserine and DNA fragmentation	21983296
M-14	Apoptosis Assay	25μM	0.5h	inhibits both the crude extract- and compound-induced apoptosis	21954959
SK-BR-3	Apoptosis Assay	50 μM	2h	blocks apoptotic DNA fragmentation induced by combination of SM-164 and radiation	21901386
MDA-MB-468	Apoptosis Assay	50 μM	2h	blocks apoptotic DNA fragmentation induced by combination of SM-164 and radiation	21901386
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Z-VAD-FMK (Z-VAD(OMe)-FMK) は、細胞透過性で不可逆的な汎カスパーゼ阻害剤であり、THP.1 および Jurkat T 細胞のアポトーシス (apoptosis) をブロックします。

特性

A key compound for apoptosis studies.

Targets

Pan-caspase ^[1]
(THP.1, Jurkat T-cells)

In Vitro
In vitro	Z-VAD-FMK (10 μM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates. Z-VAD-FMK (10 μM) inhibits the processing of CPP32 in intact THP.1 and Jurkat cells. ^[1] Z-VAD-FMK (50 μM) cotreatment abolishes the apoptotic morphology of camptothecin-treated HL60 cells. Z-VAD-FMK (50 μM) blocks camptothecin-induced DNA fragmentation in HL60 cells. ^[2] Z-VAD-FMK (50 μM) inhibits cell death following dSMN dsRNA-induced apoptosis in S2 cells. Z-VAD-FMK (50 μM) increases the percentage of transfected cells surviving from 26% to 63% in S2 cells. ^[3] Z-VAD-FMK (> 100 μM) enhances TNFα-induced neutrophil apoptosis, lower concentrations (1-30 μM) completely blocks TNFα-stimulated apoptosis in human neutrophils. ^[4] Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes. Z-VAD-FMK (10 mM) inhibits apoptosis in anterior stromal keratocytes detected with the TUNEL assay. ^[5]
細胞実験	細胞株	human granulosa cell lines (GC1a, HGL5, COV434)
	濃度	50 μM
	反応時間	48 h
	実験の流れ	To validate the efficacy of Z-VAD-FMK, three human granulosa cell lines (GC1a, HGL5, COV434) were treated for 48 h under normoxic conditions. To mimic the ischemic phase that occurs after ovarian fragment transplantation, cells were cultured without serum under hypoxia (1 % O2) and treated with Z-VAD-FMK. The metabolic activity of the cells was evaluated by WST-1 assay. Cell viability was determined by FACS analyses. The expression of apoptosis-related molecules was assessed by RT-qPCR and Western blot analyses.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3 Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II p23 / Hsp90 / hTRET / MMP2 cyto C		27191889
	Growth inhibition assay	Cell viability		12522090
	Immunofluorescence	cytochrome c Smac/DIABLO Endo G AIF		11726499
	ELISA	IL-1β		26254306

In Vivo
In Vivo	In vivo Z-VAD-FMK administration has been shown previously to be nontoxic and to prevent apoptosis in animal models. Intraperitoneal HK-GBS injection leads to preterm delivery, and pretreatment with Z-VAD-FMK delays preterm delivery in mice. In OVA-sensitized mice,treatment of z-VAD-fmk inhibits allergen-induced leukocyte infiltration. Systemic injection of the pan-caspase inhibitor z-VAD-fmk immediately before OVA challenge reduced inflammatory cell accumulation, mucus hypersecretion, and Th2 cytokine release in OVA-sensitized/challenged mice. Treatment with z-VAD-fmk blocked terminal differentiation of lens epithelial cells and keratinocytes, the differentiation of monocytes into macrophages, and the differentiation of erythroid progenitors. z-VAD-fmk attenuated allergen-induced airway inflammation and hyperreactivity. Treatment with z-VAD-fmk in vivo also prevented subsequent T cell activation ex vivo^[7].
動物実験	動物モデル	CD1 mice
	投与量	10 mg/kg
	投与経路	i.p.

参考
[1] Slee EA, et al. Biochem J, 1996, 315 (Pt 1), 21-24. [2] Shimizu T, et al. Leukemia, 1997, 11(8), 1238-1244. [3] Ilangovan R, et al. J Biol Chem, 2003, 278(33), 30993-30999. [4] Cowburn AS, et al. Blood, 2005, 105(7), 2970-2972. [5] Kim WJ, et al. Exp Eye Res, 2000, 71(3), 225-232. [6] Fransolet M, et al. J Assist Reprod Genet. 2015, 32(10):1551-9. [7] Equils O, et al. Infect Dis Obstet Gynecol. 2009, 2009:749432. + 展開

参考

+ 展開

化学情報

分子量	467.49	化学式	C₂₂H₃₀FN₃O₇
CAS No.	187389-52-2	SDF	Download Z-VAD-FMK SDFをダウンロードする
Smiles	CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 93 mg/mL ( (198.93 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 2 mg/mL

Water : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.					投与溶液組成計算機
	Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.7mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 94 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.	投与溶液組成計算機
Clear solution	2% DMSO 1 35 %PEG 300 2 2%Tween 80 3 ddH2O	2.0mg/ml (4.28mM)	Taking the 1 mL working solution as an example, add 20 μL of 100 mg/ml clear DMSO stock solution to 350 μL of PEG300, mix evenly to clarify it; add 20 μL of Tween-80 to the above system, mix evenly to clarify it; Then continue to add 610 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	2.35mg/ml (5.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 47 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

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* 大学・企業名 大学・企業名を記入してください

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電話番号

* 内容 お問い合わせ内容をご入力ください

よくある質問（FAQ）

質問1:
Can you suggest an in vivo formulation of S7023 for animal studies?

回答
For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

質問2:
What is difference between S7023 & S8102?

回答
Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

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