Caffeic Acid Phenethyl Ester

別名：CAPE, Phenylethyl Caffeate

製品コード：S7414 純度：99.99%

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate) is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

CAS No. 104594-70-9

サイズ	価格(税別)	在庫状況
50mg	JPY 22000	国内在庫あり
200mg	JPY 40500	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（50）

製品安全説明書

現在のバッチを見る：純度： 99.99%

99.99

Caffeic Acid Phenethyl Ester関連製品

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関連化合物ライブラリー	FDA-approved Drug Library Immunology/Inflammation Compound Library Small Molecule Immuno-Oncology Compound Library NF-κB Signaling Compound Library FDA-approved Anticancer Drug Library	もっと見る

シグナル伝達経路

NF-κBシグナル伝達経路

NF-κB阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
rat VSMC	Function assay	5 μM	30 mins	Decrease in intracellular ROS level in PDGF-stimulated rat VSMC at 5 uM pretreated with compound for 30 mins measured after up to 23 hrs of post stimulation using H2DCF by flow cytometric analysis	21265554
rat PC12 cells	Function assay	2.5 μM	3 h	Neuroprotective activity against H2O2-induced damage in rat PC12 cells assessed as cell viability at 2.5 uM preincubated for 3 hrs followed by H2O2 challenge measured after 5 hrs by MTT assay	24697335
mouse RAW264.7 cells	Function assay		24 h	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay, EC50=0.193 μM	18667320
mouse 26-L5 cells	Proliferation assay		72 h	Antiproliferative activity against mouse 26-L5 cells after 72 hrs by MTT assay, EC50=0.3 μM	12027739
human HeLa cells	Proliferation assay		72 h	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay, EC50=2.36 μM	12027739
mouse J774.1 cells	Function assay		24 h	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=4.8 μM	15974608
human LNCAP cells	Function assay		24 h	Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA, IC50=6.2 nM	24080105
mouse BV2 cells	Function assay		24 h	Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay, IC50=6.4 μM	23870700
mouse B16-BL6 cells	Proliferation assay		72 h	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay, EC50=6.79 μM	12027739
HUVEC cells	Cytotoxic assay		18 h	Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay	20598894
human HT1080 cells	Proliferation assay		72 h	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay, EC50=9.5 μM	12027739
human HL60 cells	Cytotoxic assay		72 h	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay, IC50=9.8 μM	20673727
human LS 174T cells	Cytotoxic assay		72 h	Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay, IC60=9.9 μM	20673727
human Bel7404 cells	Cytotoxic assay		72 h	Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay, IC50=14.5 μM	20673727
human BGC823 cells	Cytotoxic assay		72 h	Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=19.3 μM	20673727
human HO8910 cells	Cytotoxic assay		72 h	Cytotoxicity against human HO8910 cells after 72 hrs by MTT assay, IC50=25.5 μM	20673727
human A549 cells	Cytotoxic assay		72 h	Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=26.8 μM	20673727
human LNCAP cells	Cytotoxic assay		24 h	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay, IC50=33.7 μM	24080105
human ECA109 cells	Cytotoxic assay		72 h	Cytotoxicity against human ECA109 cells after 72 hrs by MTT assay, IC50=42.6 μM	20673727
human KB cells	Cytotoxic assay		72 h	Cytotoxicity against human KB cells after 72 hrs by MTT assay, IC50=45.2 μM	20673727
human K562 cells	Cytotoxic assay		72 h	Cytotoxicity against human K562 cells after 72 hrs by MTT assay, IC50=46 μM	20673727
human Bewo cells	Function assay			Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay, IC50=2.96 μM	25160837
human Bel7402 cells	Cytotoxic assay			Cytotoxicity against human Bel7402 cells by MTT assay, IC50=5.5 μM	18952420
human SGC7901 cells	Function assay			Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay, IC50=14.26 μM	25160837
human MCF7 cells	Cytotoxic assay			Cytotoxicity against human MCF7 cells by MTT assay, IC50=26.7 μM	18952420
human HeLa cells	Function assay			Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay, IC50=37.98 μM	25160837
human HepG2 cells	Function assay			Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay, IC50=40.87 μM	25160837
human BCG823 cells	Cytotoxic assay			Cytotoxicity against human BCG823 cells by MTT assay, IC50=44.6 μM	18952420
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate) is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Targets

NF-κB ^[1]

In Vitro
In vitro	Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. ^[1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. ^[2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. ^[3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. ^[4]
細胞実験	細胞株	Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
	濃度	~200 μM
	反応時間	3 days
	実験の流れ	Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO₂. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	p-p65 / CDH6 / RUNX2 / E-cadherin / N-cadherin / Vimentin / Slug / Snail Nrf2 AKT / AKT1 / AKT2 / AKT3 / p-AKT / FOXO1 / FOXO3a / p-FOXO1 / p-FOXO3a / Skp2 / p27(Kip) / Cyclin D1 / Rb / p-Rb		29284791
	Immunofluorescence	NF-κB		29284791
	Growth inhibition assay	Cell number		23615471

In Vivo
In Vivo	In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. ^[5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. ^[6]
動物実験	動物モデル	C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
	投与量	~10 mg/kg
	投与経路	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05726708	Recruiting	Autism Spectrum Disorder	Centre Hospitalier Universitaire Vaudois	October 24 2023	Not Applicable
NCT05934019	Recruiting	Mental Health Issue\|Transdiagnostic Mechanisms\|Adolescent Psychology\|Prevention\|Online Intervention\|Psychological Intervention\|Mental Disorder in Adolescence Subclinical	University of Bern	June 9 2023	Not Applicable

参考
[1] Natarajan K, et al. Proc Natl Acad Sci U S A. 1996, 93(17), 9090-9095. [2] Banskota AH, et al. J Ethnopharmacol. 2002, 80(1), 67-73. [3] Amodio R, et al. Int J Dev Neurosci. 2003, 21(7), 379-389. [4] Zhao WX, et al. Int J Mol Med. 2014, 33(3), 687-694. [5] Chung TW, et al. J Mol Med (Berl). 2013, 91(2), 271-282. [6] Park JH, et al. Int Immunopharmacol. 2004, 4(3), 429-436. + 展開

参考

+ 展開

化学情報

分子量	284.31	化学式	C₁₇H₁₆O₄
CAS No.	104594-70-9	SDF	Download Caffeic Acid Phenethyl Ester SDFをダウンロードする
Smiles	C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1个月-20°Cin solvent

In vitro
Batch:

DMSO : 56 mg/mL ( (196.96 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 56 mg/mL

Water : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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