Lycorine hydrochloride

別名：Galanthidine hydrochloride, Amarylline hydrochloride, Narcissine hydrochloride, Licorine hydrochloride

製品コード：S3800 純度：99.81%

Lycorine (Galanthidine, Amarylline, Narcissine, Licorine), a natural alkaloid extracted from the Amaryllidaceae plant family, has been reported to exhibit a wide range of physiological effects, including the potential effect against cancer. It is an HCV inhibitor with strong activity.

CAS No. 2188-68-3

サイズ	価格(税別)	在庫状況
25mg	JPY 22000	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（1）

製品安全説明書

現在のバッチを見る：純度： 99.81%

99.81

Lycorine hydrochloride関連製品

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ADC Cytotoxin阻害剤の選択性比較

生物活性

製品説明	Lycorine (Galanthidine, Amarylline, Narcissine, Licorine), a natural alkaloid extracted from the Amaryllidaceae plant family, has been reported to exhibit a wide range of physiological effects, including the potential effect against cancer. It is an HCV inhibitor with strong activity.

In Vitro
In vitro	Lycorine not only possesses strong pharmacological effects on many diseases, including anti-leukemia, anti-tumor, anti-angiogenesis, anti-virus, anti-bacteria, anti-inflammation, and anti-malaria, but also exerts many other biological functions, such as inhibition of acetylcholinesterase and topoisomerase, suppression of ascorbic acid biosynthesis, and control of circadian period length. Lycorine inhibits cell proliferation and induces cell apoptosis in acute myeloid leukemia (AML) cell line HL-60, monocyte leukemia cell line U937, and T-cell leukemia cell line Jurkat. Lycorine also blocks multiple myeloma KM3 cell cycle, induces K562 cell-cycle arrest at the G0/G1 phase and suppresses tumorigenesis and the growth of various tumor cells, including melanoma C8161, ovarian cancer Hey1B, lung cancer A549, glioblatatoma U373, and esophageal cancer OE21^[1]. Lycorine can inhibit protein synthesis in eukaryotic cells, and acetylcholinesterase activity^[2].
細胞実験	細胞株	K562 cells
	濃度	2.5 or 5.0 μM
	反応時間	24 h
	実験の流れ	--

In Vivo
In Vivo	Lycorine exhibits its numerous pharmacological effects on various diseases with very low toxicity and mild side effects. In vivo studies, lycorine effectively inhibits tumor growth in several mouse xenograft models. It effectively inhibits tumor neovascularization. Lycorine has trivial toxicity to normal cells and normal bodies. The animal toxicological experiments show that the 50% lethal death rate (LD50) of lycorine in mice is 112.2±0.024 mg/kg via intraperitoneal injection. The LD50 of lycorine in mice is 344 mg/kg via gastric lavage injection, suggesting that lycorine is very low toxicity in gastrointestinal administration. In vivo studies show that lycorine has linear plasma kinetics with a mean elimination half-life of 0.67 and 0.3 h after single s.c. and i.v. administration, respectively. The mean oral bioavailability is calculated to be about 40%. Biochemical and hematological parameters of safety studies indicate no pathological signs^[1].
動物実験	動物モデル	SCID mice inoculated with HL-60 cells
	投与量	5 or 10 mg/kg/day
	投与経路	i.p.

参考
[1] ZhiFei Cao, et al. Science China Chemistry. 2013, 56(10): 1382–1391. [2] Liu J, et al. Biomed Pharmacother. 2007, 61(4):229-34. [3] Li L, et al. Cancer Cell Int. 2012, 12(1):49.

参考

化学情報

分子量	323.77	化学式	C₁₆H₁₇NO₄.HCl
CAS No.	2188-68-3	SDF	Download Lycorine hydrochloride SDFをダウンロードする
Smiles	C1CN2CC3=CC4=C(C=C3C5C2C1=CC(C5O)O)OCO4.Cl
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

DMSO : 65 mg/mL ( (200.75 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo Batch: Add solvents to the product individually and in order.					投与溶液組成計算機
	Clear solution	5% DMSO 1 95% Corn oil	0.5mg/ml (1.54mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	投与溶液組成計算機
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.25mg/ml (10.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.8mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.25mg/ml (10.04mM)	Taking the 1 mL working solution as an example, add 50 μL of 65 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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