Isoimperatorin

Isoimperatorin significantly inhibits the proliferation of SGC-7901 cells in a dose- and time-dependent manner, and the half maximal inhibitory concentration is 18.75 µg/ml. The expression levels of the pro-apoptotic proteins, Bax, c-caspase-3 and c-caspase-9, are significantly increased and the expression levels of the anti-apoptotic proteins, Survivin and Bcl-2, are significantly reduced after the treatment^[1].

The antiproliferative activity of isoimperatorin against SGC-7901 cells was evaluated using an MTT reduction assay. The SGC-7901 cells were seeded on 96-well culture plates with RPMI-1640 medium at a density of 5×10³ cells/well. After 24 h of incubation at 37°C in 5% CO2/95% air, the cells were treated at different concentrations of isoimperatorin (5, 10, 15, 20, 25, 30, 35 and 40 µg/ml) and 0.05% DMSO (control) for 48 h to examine dose-dependency; or with isoimperatorin at a concentration of 20 µg/ml and 0.05% DMSO (control) for 12, 24, 36, 48, 60 and 72 h to examine time-dependent effects. Subsequently, 20 µl MTT (5 mg/ml) was separately added into each well, and the cells were cultured at 37°C in 5% CO2/95% air for another 3 h. Subsequently, 200 µl DMSO was separately added into each well and the optical density (OD) of the DMSO solution was measured at 570 nm using a microplate reader.

Isoimperatorin significantly inhibits the growth of SGC-7901 cell-induced tumor in vivo by increasing the expression levels of pro-apoptotic proteins (Bax, c-caspase-3 and c-caspase-9) and reducing the expression levels of anti-apoptotic proteins (Survivin and Bcl-2) without adverse effects on the increasing body weight of nude mice^[1].