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DMOG

別名：Dimethyloxalylglycine

DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.Solutions are unstable and should be fresh-prepared.

DMOG化学構造

CAS No. 89464-63-1

サイズ 価格(税別) 在庫状況
JPY 22000 国内在庫あり
JPY 49000 国内在庫なし(納期7~10日)
JPY 145500 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール：[email protected]
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製品安全説明書

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DMOG関連製品

シグナル伝達経路

Hydroxylase Signaling Pathways

Hydroxylaseシグナル伝達経路

Hydroxylase阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
human 293T cells  Function assay 2.5 mM Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM 19359167
human SW982 cell Growth inhibition assay Inhibition of human SW982 cell growth in a cell viability assay, IC50=33.7007 μM SANGER
human OPM-2 cell Growth inhibition assay Inhibition of human OPM-2 cell growth in a cell viability assay, IC50=28.8227 μM SANGER
human KU812 cell Growth inhibition assay Inhibition of human KU812 cell growth in a cell viability assay, IC50=28.4579 μM SANGER
human CAL-12T cell Growth inhibition assay Inhibition of human CAL-12T cell growth in a cell viability assay, IC50=26.5502 μM SANGER
human COR-L105 cell Growth inhibition assay Inhibition of human COR-L105 cell growth in a cell viability assay, IC50=26.4436 μM SANGER
human LC-2-ad cell Growth inhibition assay Inhibition of human LC-2-ad cell growth in a cell viability assay, IC50=24.5573 μM SANGER
human YT cell Growth inhibition assay Inhibition of human YT cell growth in a cell viability assay, IC50=23.3984 μM SANGER
human KYSE-410 cell Growth inhibition assay Inhibition of human KYSE-410 cell growth in a cell viability assay, IC50=20.8197 μM SANGER
human CAL-39 cell Growth inhibition assay Inhibition of human CAL-39 cell growth in a cell viability assay, IC50=19.3277 μM SANGER
human DMS-273 cell Growth inhibition assay Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=15.965 μM SANGER
human BL-70 cell Growth inhibition assay Inhibition of human BL-70 cell growth in a cell viability assay, IC50=15.9423 μM SANGER
human A3-KAW cell Growth inhibition assay Inhibition of human A3-KAW cell growth in a cell viability assay, IC50=15.8174 μM SANGER
human U-698-M cell Growth inhibition assay Inhibition of human U-698-M cell growth in a cell viability assay, IC50=13.3306 μM SANGER
human KS-1 cell Growth inhibition assay Inhibition of human KS-1 cell growth in a cell viability assay, IC50=11.2557 μM SANGER
human LOUCY cell Growth inhibition assay Inhibition of human LOUCY cell growth in a cell viability assay, IC50=10.3493 μM SANGER
human LB996-RCC cell Growth inhibition assay Inhibition of human LB996-RCC cell growth in a cell viability assay, IC50=5.6877 μM SANGER
human SR cell Growth inhibition assay Inhibition of human SR cell growth in a cell viability assay, IC50=4.57946 μM SANGER
human HH cell Growth inhibition assay Inhibition of human HH cell growth in a cell viability assay, IC50=34.0235 μM SANGER
human RS4-11 cell Growth inhibition assay Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=34.1806 μM SANGER
human BEN cell Growth inhibition assay Inhibition of human BEN cell growth in a cell viability assay, IC50=36.8187 μM SANGER
human CCRF-CEM cell Growth inhibition assay Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=37.7729 μM SANGER
human EW-13 cell Growth inhibition assay Inhibition of human EW-13 cell growth in a cell viability assay, IC50=39.0481 μM SANGER
human NCI-H2228 cell Growth inhibition assay Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=42.818 μM SANGER
human KE-37 cell Growth inhibition assay Inhibition of human KE-37 cell growth in a cell viability assay, IC50=43.2915 μM SANGER
human A4-Fuk cell Growth inhibition assay Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=45.0364 μM SANGER
human MZ1-PC cell Growth inhibition assay Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50=45.9888 μM SANGER
human MLMA cell Growth inhibition assay Inhibition of human MLMA cell growth in a cell viability assay, IC50=46.3292 μM SANGER
human EB-3 cell Growth inhibition assay Inhibition of human EB-3 cell growth in a cell viability assay, IC50=49.5792 μM SANGER
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生物活性

製品説明 DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor and inhibitor for HIF prolylhydroxylase, leading to stabilisation and accumulation of HIF-1α protein in the nucleus. DMOG enhances autophagy.Solutions are unstable and should be fresh-prepared.
Targets
HIF prolyl hydroxylase [1]
In Vitro
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. [1] DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. [3]
細胞実験 細胞株 VSMC cells
濃度 ~100 μM
反応時間 72-96 hours
実験の流れ

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
実験結果図 Methods Biomarkers 結果図 PMID
Western blot HIF-1α p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM WNT11 / HIF-2α 29555474
Immunofluorescence HIF1α 28955984
In Vivo
In Vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. [2] Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. [4]
動物実験 動物モデル Mice (C57Bl6)
投与量 8 mg in 0.5 ml saline
投与経路 i.p.

化学情報

分子量 175.14 化学式

C6H9NO5
CAS No. 89464-63-1 SDF Download DMOG SDFをダウンロードする
密度 1.246 g/mL
Smiles COC(=O)CNC(=O)C(=O)OC
保管 3 years -20°C powder 溶液状態は不安定なので使用直前に調整してください。少量づつ分包して保管し、都度使い切る事が推奨されます。

In vitro
Batch:

DMSO : 35 mg/mL ( (199.84 mM)； 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 35 mg/mL

Ethanol : 35 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

mg/kg g μL 匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH2O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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