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Aloperine

Aloperine is an isolated alkaloid in sophora plants such as Sophora alopecuroides L, and exhibits anti-inflammatory, antibacterial, antiviral, and anti-tumor properties.

Aloperine化学構造

CAS No. 56293-29-9

サイズ 価格(税別) 在庫状況
JPY 15900 国内在庫あり
JPY 101400 国内在庫なし(納期7~10日)

代表番号: 045-509-1970|電子メール：[email protected]
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Aloperine関連製品

Antiviral阻害剤の選択性比較

生物活性

製品説明 Aloperine is an isolated alkaloid in sophora plants such as Sophora alopecuroides L, and exhibits anti-inflammatory, antibacterial, antiviral, and anti-tumor properties.
In Vitro
In vitro Compared with other alkaloids including sophoridine, sophocarpine, matrine, oxymatrine and cytisine, Aloperine treatment exerts the most potent cytotoxic activity against human cancer cell lines of differing tissue origins, including leukaemia cell lines HL-60, U937 and K562, oesophageal cancer EC109 cells, lung cancer A549 cells and the hepatocellular carcinoma HepG2 cell line with IC50 of 0.04 mM, 0.27 mM, 0.36 mM, 1.11 mM, 1.18 mM, and 1.36 mM, respectively. The strongest cytotoxic effect of Aloperine on HL-60 cells is observed at 72 hours with the inhibition rate of 94.1%.In contrast to the effect on leukaemia cells, up to 1 mM of Aloperine does not significantly reduce the viability of normal PBMNCs at 72 hours. Aloperine treatment at 20 μM for 48 hours significanlty induces apoptosis and autophagy in HL-60 cells in a dose-dependent manner. [2]
細胞実験 細胞株 K562, U937, HL-60, EC109, A549 and HepG2
濃度 Dissolved in DMSO, final concentrations ~2 mM
反応時間 24, 48, and 72 hours
実験の流れ Cells are seeded in 96-well plates in 100-μL culture medium. After incubation of 4 hours for leukaemia cells and of 24 hours for solid cancer cells, experimental media containing either excipient control or Aloperine are added to appropriate wells. Five concentrations of Aloperine for 48-hour treatment are used to determine the in vitro IC50 growth inhibitory values of Aloperine in cancer cells. After incubation, 10 μL of MTT solution (5 mg/mL) is added to each well. The plates are then incubated for 4 hours at 37 °C. Intracellular formazan crystals are dissolved by addition of 100 μL of isopropanol-HCI-SDS solution to each well. After an overnight incubation at 37 °C, the optical density of the samples is determined at 570 nm. DNA fragmentation is analysed after the extraction of DNA from cells exposed to the indicated doses of Aloperine for 48 hours using apoptotic DNA ladder kit. For autophagy detection, cells are collected and incubated with PBS containing 5 μM acridine orange for 15 minutes. The acridine orange is removed and the cells are resuspended in 100 μL of PBS. Fluorescent micrographs are obtained with an inverted fluorescent microscope. Autophagy is quantified based on the mean number of cells displaying intense red staining for three fields (containing at least 50 cells per field) for each experimental condition.
In Vivo
In Vivo Topical application of 1% Aloperine suppresses 2, 4-dinitrofluorobenzene (DNFB)-induced increase in ear thickness and ear erythema in BALB/c mice, and significantly decreases the up-regulated mRNA and protein levels of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) induced by DNFB in ear biopsy homogenates. [1] Topical application of Aloperine reduces the DNFB-induced dermatitis (dermatitis index and ear thickness) in NC/Nga mice at day 13 and day 14 in a dose-dependent manner. Aloperine treatment reduces DNFB-induced lymphocytes infiltration and eosinophils infiltration in a dose-dependent manner. Aloperine treatment also reduces the DNFB-induced infiltration of mast cells in a dose-dependent manner. Aloperine reduces the plasma level of IgE in a dose-dependent manner. Aloperine markedly reduces DNFB-induced increase in IL-4, IL-13 and IFN-γ productions, while it increases the IL-10 level in a dose-dependent manner. Aloperine treatment significantly reduces the cytokine levels of TNF-α, IL-1β and IL-6 in ear biopsies homegenates of NC/Nga mice in a dose-dependent manner. [3]
動物実験 動物モデル Female BALB/c mice with DNFB-induced allergic contact dermatitis
投与量 1% (w/w)
投与経路 Topical application

化学情報

分子量 232.36 化学式

C15H24N2
CAS No. 56293-29-9 SDF Download Aloperine SDFをダウンロードする
Smiles C1CCN2CC3CC(C2C1)C=C4C3NCCC4
保管

In vitro
Batch:

DMSO : 46 mg/mL ( (197.96 mM)； 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 46 mg/mL

Water : 4 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

mg/kg g μL 匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH2O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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