Acetylcysteine (N-acetylcysteine)

別名：N-acetylcysteine

製品コード：S1623 純度：99.97%

Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine) is a ROS(reactive oxygen species) inhibitor that antagonizes the activity of proteasome inhibitors. It is also a tumor necrosis factor production inhibitor. Acetylcysteine(N-acetyl-l-cysteine) suppresses TNF-induced NF-κB activation through inhibition of IκB kinases. Acetylcysteine(N-acetyl-l-cysteine) induces apoptosis via the mitochondria-dependent pathway. Acetylcysteine(N-acetyl-l-cysteine) inhibits ferroptosis and virus replication.Solutions are unstable and should be fresh-prepared.

CAS No. 616-91-1

サイズ	価格(税別)	在庫状況
500mg	JPY 22000	国内在庫あり
1g	JPY 29500	国内在庫あり
5g	JPY 59500	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（203）

製品安全説明書

現在のバッチを見る：純度： 99.97%

99.97

Acetylcysteine (N-acetylcysteine)関連製品

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シグナル伝達経路

TNF-alphaシグナル伝達経路

TNF-alpha阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
HUVEC cells	Function assay	2μM,4μM,8μM	90min	HUVECs that were exposed with serum of P. falciparum and treated with NAC 2 µM，4 µM，8 µM. Supernatant from culture and lysed cells culture were measured for H2O2, GSH and MDA levels.	21602763
HUVEC cells	Function assay	10μM	2h	HUVECs were pretreated with 10 μM NAC for 2 hours, and then treated with 0.5 μM PCB 118. presentce of NAC for 48 hours.	28592194
NHBE	Cytotoxicity assay	1 mM	18 hrs	Cytoprotective activity in NHBE cells assessed as inhibition of tBHP-induced GSH depletion at 1 mM preincubated for 18 hrs followed by tBHP addition for 4 hrs by thiostar dye based fluorescence assay	27031670
HUVEC	Antioxidant assay	5 mmol/L	4 hrs	Antioxidant activity against H2O2-induced lipid accumulation in HUVEC cells assessed as reduction in MDA level at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs	22841280
HUVEC	Antioxidant assay	5 mmol/L	4 hrs	Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs	22841280
HUVEC	Cytotoxicity assay	5 mmol/L	4 hrs	Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay	22841280
HUVEC	Cytotoxicity assay	5 mmol/L	4 hrs	Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as LDH release at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by LDH assay	22841280
SH-SY5Y	Neuroprotective assay	5 mM	4 hrs	Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as upregulation of Bax expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis	23403085
SH-SY5Y	Neuroprotective assay	5 mM	4 hrs	Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as downregulation of Bcl2 expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis	23403085
HT22	Neuroprotective assay	5 mM	24 hrs	Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as increase in cell viability at 5 mM after 24 hrs by MTT assay	29122481
HT22	Neuroprotective assay	50 uM	10 to 12 hrs	Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as reduction in apoptotic cells at 50 uM after 10 to 12 hrs by Annexin V-alexa 488/propidium iodide staining based flow cytometry	29122481
HL-7702	Hepatoprotective assay	10 uM	24 hrs	Hepatoprotective activity against APAP-induced cell injury in human HL-7702 cells assessed as increase in survival rate at 10 uM pre-incubated for 24 hrs before APAP addition and measured 6 hrs post APAP challenge by MTT assay	28729056
PC12	Cytotoxicity assay		24 hrs	Inhibition of 6-hydroxydopamine induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level	17158454
BL21 (DE3)	Function assay		30 mins	Inhibition of hexahistidine-tagged IMP-7 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using fluorocillin as substrate incubated for 30 mins by TECAN fluorescent plate reader analysis, IC50 = 20.7 μM.	25815530
PC12	Cytotoxicity assay		24 hrs	Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level	17158454
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明

Targets

NF-κB ^[8]

Ferroptosis ^[9]

ROS ^[6]

TNF-α ^[7]

In Vitro
In vitro	N-acetylcysteine inhibits activation of c-Jun N-terminal kinase, p38 MAP kinase and redox-sensitive activating protein-1 and nuclear factor kappa B transcription factor activities regulating expression of numerous genes. N-acetylcysteine can also prevent apoptosis and promote cell survival by activating extracellular signal-regulated kinase pathway, a concept useful for treating certain degenerative diseases. N-acetylcysteine directly modifies the activity of several proteins by its reducing activity. ^[1] N-acetylcysteine prevents apoptotic DNA fragmentation and maintains long-term survival in the absence of other trophic support in serum-deprived PC12 cells. N-acetylcysteine also prevents death of PC12 cells and sympathetic neurons. ^[2] N-acetylcysteine causes dose-dependent reductions in viability in rat and human aortic smooth muscle cells. ^[3] N-acetylcysteine activates the Ras-extracellular signal-regulated kinase (ERK) pathway in PC12 cells. N-acetylcysteine protects neuronal cells from death evoked by withdrawal of trophic support. N-acetylcysteine increases nitric oxide (NO) release from protein-bound stores in vascular tissue. N-acetylcysteine pretreatment of PC12 cells interferes with NGF-dependent signaling and neurite outgrowth, and it was suggested that NAC interferes with redox-sensitive steps in the NGF mechanism. ^[4]
細胞実験	細胞株	MCF cells
	濃度	5 mM
	反応時間	24 h
	実験の流れ	Cells were treated with indicated concentration of drug for 24 h.

In Vivo
In Vivo	N-acetylcysteine improves cognition of 12-month-old SAMP8 mice in both the T-maze footshock avoidance paradigm and the lever press appetitive task without inducing non-specific effects on motor activity, motivation to avoid shock, or body weight. ^[5]
動物実験	動物モデル	Male SAMP8 mice
	投与量	100 mg/kg
	投与経路	s.c.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT04520139	Not yet recruiting	Ovarian Cancer\|Cognitive Impairment	University of California Irvine\|Jarrow Formulas Inc	December 2024	Phase 1\|Phase 2
NCT06112834	Not yet recruiting	Botulism	California Department of Public Health	June 2024	Phase 2
NCT06260566	Not yet recruiting	Biliary Atresia	Sanjiv Harpavat\|Baylor College of Medicine	May 2024	Phase 1
NCT06377410	Not yet recruiting	Chronic Obstructive Pulmonary Disease	National University of Malaysia	May 1 2024	Not Applicable
NCT06223568	Not yet recruiting	Squamous Cell Carcinoma of the Head and Neck\|Oropharynx\|Human Papillomavirus Viruses\|Drug Therapy\|Cancer Vaccine	National Cancer Institute (NCI)\|National Institutes of Health Clinical Center (CC)	May 15 2024	Phase 2

参考
[1] Alvirez-Freites EJ, et al. Antimicrob Agents Chemother, 2002, 46(4), 1022-1025. [2] Ferrari G, et al. J Neurosci, 1995, 15(4), 2857-2866. [3] Tsai JC, et al. J Biol Chem, 1996, 271(7), 3667-3670. [4] Yan CY, et al. J Neurosci, 1998, 18(11), 4042-4049. [5] Farr SA, et al. J Neurochem, 2003, 84(5), 1173-1183. [6] Halasi M, et al. Biochem J. 2013, 454(2):201-8. [7] Peristeris P, et al. Cell Immunol. 1992, 140(2):390-9. [8] Xiaoqi Pan, et al. Toxicol Lett . 2018 May 15;288:55-64. [9] Saravanan S Karuppagounder, et al. Ann Neurol . 2018 Dec;84(6):854-872. [10] Zhang Z, et al. EMBO Mol Med. 2022 Feb 7;14(2):e14903. + 展開

参考

+ 展開

化学情報

分子量	163.19	化学式	C₅H₉NO₃S
CAS No.	616-91-1	SDF	Download Acetylcysteine (N-acetylcysteine) SDFをダウンロードする
Smiles	CC(=O)NC(CS)C(=O)O
保管	3 years -20°C powder	溶液状態は不安定なので使用直前に調整してください。少量づつ分包して保管し、都度使い切る事が推奨されます。

In vitro
Batch:

DMSO : 32 mg/mL ( (196.09 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 32 mg/mL

Ethanol : 32 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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