ホームページ Epigenetics Histone Acetyltransferase inhibitor - NF-κB inhibitor - HDAC inhibitor - Nrf2 activator - Influenza Virus inhibitor - HIV inhibitor - Autophagy activator - Apoptosis related activator - Ferroptosis inhibitor - Epigenetic Reader Domain inhibitor - HCV inhibitor Curcumin For research use only.

Curcumin

別名：Diferuloylmethane, Natural Yellow 3, Turmeric yellow

Curcumin (Diferuloylmethane, Natural Yellow 3, Turmeric yellow) is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase（IC50~25 μM）and Histone deacetylase (HDAC); activates Nrf2 pathway and supresses the activation of NF-κB. Curcumin induces mitophagy, autophagy, apoptosis, and cell cycle arrest with antitumor activity. Curcumin reduces renal damage associated with rhabdomyolysis by decreasing ferroptosis-mediated cell death. Curcumin exhibits anti-infective properties against various human pathogens like the influenza virus, hepatitis C virus, HIV and so on.

Curcumin化学構造

CAS No. 458-37-7

サイズ 価格(税別) 在庫状況
10mM (1mL in DMSO) JPY 29500 国内在庫あり
JPY 22000 国内在庫あり
JPY 70500 国内在庫あり

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よく尋ねられる質問

製品安全説明書

現在のバッチを見る： 純度： 99.94%
99.94

Curcumin関連製品

シグナル伝達経路

Histone Acetyltransferase Signaling Pathways

Histone Acetyltransferaseシグナル伝達経路

Histone Acetyltransferase阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
UMUC3 Cell viability assay 0.1 or 0.4 μg/ml 24, 48, and 73 h Curcumin alone, added at a concentration from 0.1 to 0.4 μg/ml, did not alter cell growth of RT112 cells, slightly reduced UMUC3 cell growth at 0.4 μg/ml, and only moderately suppressed growth of TCCSUP at 0.2 and 0.5 μg/ml, each compared to respective controls 30984278
RT112 Cell viability assay 0.1 or 0.4 μg/ml 24, 48, and 72 h Curcumin alone, added at a concentration from 0.1 to 0.4 μg/ml, did not alter cell growth of RT112 cells, slightly reduced UMUC3 cell growth at 0.4 μg/ml, and only moderately suppressed growth of TCCSUP at 0.2 and 0.4 μg/ml, each compared to respective controls 30984278
U87 Cell viability assay 0, 10, 20, 30, 40, and 50 µM/L 24 hours At concentrations higher than 20 µM/L, cell viabilities steeply declined 31062527
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生物活性

製品説明 Curcumin (Diferuloylmethane, Natural Yellow 3, Turmeric yellow) is the principal curcuminoid of the popular Indian spice turmeric, which is a member of the ginger family (Zingiberaceae). It is an inhibitor of p300 histone acetylatransferase（IC50~25 μM）and Histone deacetylase (HDAC); activates Nrf2 pathway and supresses the activation of NF-κB. Curcumin induces mitophagy, autophagy, apoptosis, and cell cycle arrest with antitumor activity. Curcumin reduces renal damage associated with rhabdomyolysis by decreasing ferroptosis-mediated cell death. Curcumin exhibits anti-infective properties against various human pathogens like the influenza virus, hepatitis C virus, HIV and so on.
Targets
Nrf2 [1]
(Cell-free assay)		 Ferroptosis [9] HDAC [7]
(Cell-free assay)		 NF-κB [8]
(Cell-free assay)		 p300 [6]
(Cell-free assay)
~25 μM
In Vitro
In vitro

Curcumin induces the expression of forkhead box protein O1 (FOXO1) through activation of extracellular signal-regulated kinase 1/2 signaling. Curcumin inhibits cell proliferation, which was associated with upregulation of the cyclin-dependent kinase inhibitors, p27 and p21, and downregulation of cyclin D1[2]. Curcumin induces endoplasmic reticulum (ER) stress and mitochondrial dysfunction as evidenced by up-regulation of CCAAT/enhancer binding protein homologous protein (CHOP), phosphorylation of JNK and down-regulation of SERCA2ATPase, release of cytochrome c, decrease of Bcl-2 and reduction of mitochondrial membrane potential in both AGS and HT-29 cells[3].
細胞実験 細胞株 murine melanoma cell subline(B16-R)
濃度 0-100 μM
反応時間 24-48 h
実験の流れ

1×104 B16-R cells are cultivated as monolayer culture for 12 hr. They were then incubated in 200 μL of RPMI, 10% FBS containing curcumin at final concentrations from 1–100 μM in 96-multiwell plates for 24-48 hr. After these incubations, cells are washed twice in PBS and 500 μl of fresh culture medium containing MTT (0.3 mg/mL) are added for colorimetric assay.
実験結果図 Methods Biomarkers 結果図 PMID
Western blot p-IRE1α / IREα HDAC1/ HDAC2 p-STAT3 / STAT3 29901626
Immunofluorescence β-catenin PKD1 EGFR / LC3 22523587
Growth inhibition assay Cell proliferation Cell viability 22523587
In Vivo
In Vivo

Chronic treatment with curcumin significantly reverses the CMS-induced behavioral abnormalities in stressed rats. Additionally, curcumin effectively inhibits cytokine gene expression at both the mRNA and the protein level and reduces the activation of NF-κB[4].
動物実験 動物モデル Female B6D2F1 mice (6-8 weeks old)
投与量 25 mg/kg
投与経路 i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06053411 Not yet recruiting
Interaction
Washington State University
 January 1 2024 Early Phase 1
NCT05947513 Not yet recruiting
Cervical Cancer
Addis Ababa University|Center for Innovative Drug Development and Therapeutic Trials for Africa Addis Ababa University|Akay Natural Ingredients Private Limited
 October 2023 Phase 1|Phase 2
NCT05966441 Not yet recruiting
Chemotherapy-induced Peripheral Neuropathy
Ain Shams University
 August 30 2023 Phase 2
NCT05774704 Recruiting
Bioavailability|Gut Microbiome|Safety
Texas Tech University Health Sciences Center
 August 21 2023 Phase 1|Phase 2

化学情報

分子量 368.38 化学式

C21H20O6
CAS No. 458-37-7 SDF Download Curcumin SDFをダウンロードする
Smiles COC1=C(C=CC(=C1)C=CC(=O)CC(=O)C=CC2=CC(=C(C=C2)O)OC)O
保管

In vitro
Batch:

DMSO : 250 mg/mL ( (678.64 mM)； 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機
Clear solution
5%DMSO 40% 5% 50%ddH2O
10.0mg/ml (27.15mM) Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

mg/kg g μL 匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH2O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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