Ipilimumab (anti-CTLA-4)

製品コードA2001 別名: MDX-010, BMS-734016

製品コード：A2001 純度：99.28%

Ipilimumab is an immunomodulatory monoclonal antibody directed against the cell surface antigen CTLA-4 and also a type of immune checkpoint inhibitor. MW : 148 kD.

サイズ	価格(税別)	在庫状況
1mg	JPY 44500	国内在庫あり
1mg*5	JPY 146500	お問い合わせ
5mg	JPY 146500	国内在庫あり
1mg*25	JPY 445500	お問い合わせ

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（15）

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質量管理及び製品安全説明書

現在のバッチを見る：純度： 99.28% タンパク質濃度： 4.2mg/ml 内毒素： ≤1 EU/mg

99.28

Immunology & Inflammation related阻害剤の選択性比較

生物活性

製品説明

Ipilimumab is an immunomodulatory monoclonal antibody directed against the cell surface antigen CTLA-4 and also a type of immune checkpoint inhibitor. MW : 148 kD.

同型

Human IgG1

Targets

CTLA-4 ^[1]
(Cell-free assay)

In Vitro
In Vitro	Pembrolizumab plus ipilimumab and nivolumab plus ipilimumab with chemotherapy have been approved by FDA for the first-line treatment of NSCLC patients.^[1]
細胞実験	For the intracellular cytokine analysis of TILs, cells from dissociated tumors in the density range of 1×10⁵ to 1×10⁶ cells were cultured in a 96-well U-bottom plate containing 10 µg/mL of pembrolizumab, 10 µg/mL of ipilimumab, soluble 50 ng/mL of anti-CD3, and 50 ng/mL of anti-CD28 for 24 h at 37°C, followed by the addition of brefeldin A and monensin. To measure interferon (IFN)-γ and tumor necrosis factor (TNF) secretion levels by CD8⁺ TILs according to PD-1 expression on anti-CD3 and anti-CD28 stimulation, the pre-incubation step without brefeldin A and monensin for 24 h was omitted. After 12 h, the cells were harvested and stained. Intracellular cytokines were stained after surface staining, fixation, and permeabilization with Foxp3 fixation/permeabilization solution.

In Vivo
In Vivo	⁶⁴Cu-labeled ipilimumab may be used to noninvasively monitor CTLA-4-expressing tissues in vivo.^[2]
動物試験	AnimalModels:Female athymic nude mice of human lung adenocarcinoma xenograft model Formulation:buffer-exchanged to PBS, and conjugated with DOTA for radiolabeling with ⁶⁴Cu (t1/2 = 12.7 h) Dosages:~1 mg/mL Administration:i.v.^[2]

In Vivo

⁶⁴Cu-labeled ipilimumab may be used to noninvasively monitor CTLA-4-expressing tissues in vivo.^[2]

動物試験

AnimalModels:Female athymic nude mice of human lung adenocarcinoma xenograft model
Formulation:buffer-exchanged to PBS, and conjugated with DOTA for radiolabeling with ⁶⁴Cu (t1/2 = 12.7 h)
Dosages:~1 mg/mL
Administration:i.v.^[2]

参考
[1] Kim CG, et al. J Immunother Cancer. 2021 Dec;9(12):e002780. [2] Ehlerding EB, et al. Mol Pharm. 2017 May 1;14(5):1782-1789.

参考

製品説明

CAS No.	477202-00-9
Formulation	PBS buffer, pH 7.2
Isotype	Human IgG1
Source	CHO cells
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Shipping	Shipped under low temperature conditions

各種クローンの論文数比較

^*Selleckは、市場の主要なクローンを収集し、2024年9月までの文献での使用状況を調査しました。その結果、Selleckの選定クローンが最も広く使用されていることが示されました。

技術サポート

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Handling Instructions

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* 必須

よくある質問（FAQ）

質問：1：
How to store the antibody?

回答：
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.