Puromycin 2HCl

別名：CL13900 2HCl

製品コード：S7417 純度：99.23%

Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

CAS No. 58-58-2

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 29500	国内在庫あり
25mg	JPY 22000	国内在庫あり
50mg	JPY 37000	国内在庫あり
100mg	JPY 55500	国内在庫あり
500mg	JPY 145500	国内在庫あり

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（184）

製品安全説明書

現在のバッチを見る：純度： 99.23%

99.23

Puromycin 2HClと併用されることが多い化合物

Blasticidin S HCl

Puromycin and Blasticidin S inhibits the proliferation and cell viability of metastatic castration-resistant prostate cancer (mCRPC) cells PC3/DU145.

Oseni SO, et al. bioRxiv (2021): 2021-08.

Melatonin

Melatonin potentiates puromycin-induced apoptosis with caspase-3 and AMPK activation in HL-60 cells.

Koh W, et al. J Pineal Res. 2011 May;50(4):367-73.

Puromycin 2HCl関連製品

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関連化合物ライブラリー	Anti-infection Compound Library Antibiotics compound Library Antiviral Compound Library Anti-parasitic Compound Library Gut Microbial Metabolite Library	もっと見る

Antineoplastic and Immunosuppressive Antibiotics阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Incubation Time	活性情報	PMID
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明	Puromycin 2HCl is an aminonucleoside antibiotic derived from Streptomyces alboniger. It is an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinated expression of genes during cell differentiation.

In Vitro
In vitro	The antibiotic puromycin is produced by the actinoniycete, Streptornyces alboniger, and has been used as a tool for studying protein synthesis in a number of systems. ^[1] Puromycin can be used for the selection of recombinant cells from noncultured cells. ^[2]
細胞実験	細胞株	HepG2 cells
	濃度	1.6 μM
	反応時間	72 h
	実験の流れ	Cells were treated with different concentrations of drug for 72 h.
実験結果図	Methods	Biomarkers	結果図	PMID
	Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
	Growth inhibition assay	Cell viability		31022952

In Vivo
In Vivo	Puromycin (aminonucleoside) inhibits protein synthesis leading to proteinuria & glomerulosclerosis/nephrosis.^[4]
動物実験	動物モデル	Male Sprague-Dawley rats
	投与量	5 mg/100 g
	投与経路	i.v.

参考
[1] Darken MA. Pharmacol Rev. 1964, 16, 223-243. [2] Watanabe S, et al. Biol Reprod. 2005, 72(2), 309-315. [3] Chan C, et al. Pharmacol Res Perspect. 2017 Oct;5(5):e00359. [4] Diamond JR, et al. Am J Pathol. 1986 Mar;122(3):481-7. [5] Aviner R. Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083. [6] Manabe I, et al. Circ Res. 2001;88(11):1127-1134. + 展開

参考

+ 展開

化学情報

分子量	544.43	化学式	C₂₂H₂₉N₇O₅.2HCl
CAS No.	58-58-2	SDF	Download Puromycin 2HCl SDFをダウンロードする
Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (183.67 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : 100 mg/mL

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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