Prostaglandin E2 (PGE2)

別名：Dinoprostone

製品コード：S3003 純度：97%

Prostaglandin E2 (PGE2, Dinoprostone) plays important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts.

CAS No. 363-24-6

サイズ	価格(税別)	在庫状況
10mM (1mL in DMSO)	JPY 29500	国内在庫あり
5mg	JPY 22000	国内在庫あり
25mg	JPY 43500	国内在庫あり
100mg	JPY 113500	国内在庫あり
1g	JPY 295500	国内在庫なし(納期7~10日)

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（20）

質量管理及び製品安全説明書

現在のバッチを見る： 純度：>97%

Prostaglandin E2 (PGE2)関連製品

関連化合物ライブラリー	FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Exosome Secretion Related Compound Library Human Hormone Related Compound Library	もっと見る

PGES阻害剤の選択性比較

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	活性情報	PMID
CHO	Function assay	100 nM		Activity at recombinant EP1 receptor (unknown origin) expressed in CHO cells co-expressing Gq protein at 100 nM by electrical cell substrate impedance sensing system	25701254
CHO	Function assay	100 nM		Activity at recombinant EP4 receptor (unknown origin) expressed in CHO cells co-expressing Gs protein at 100 nM by electrical cell substrate impedance sensing system	25701254
CHO	Function assay	100 nM		Activity at recombinant EP3 receptor (unknown origin) expressed in CHO cells co-expressing Gi protein at 100 nM by electrical cell substrate impedance sensing system	25701254
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP4 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.0031 μM.	22119471
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP3 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.005 μM.	22119471
CHO	Function assay		20 mins	Displacement of [3H]-PGE2 from mouse EP1 receptor expressed in CHO cells after 20 mins by liquid scintillation counting, Ki = 0.006 μM.	22119471
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP2 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.022 μM.	22119471
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP4 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.0031 μM.	22386979
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP3 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.005 μM.	22386979
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP1 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.006 μM.	22386979
CHO	Function assay		60 mins	Displacement of [3H]-PGE2 from mouse EP2 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.022 μM.	22386979
CHEM1	Function assay		2 hrs	Displacement of [3H]PGE2 from human recombinant prostanoid EP4 receptor in CHEM1 cells after 2 hrs, Ki = 0.00045 μM.	23403082
CHEM1	Function assay		2 hrs	Displacement of [3H]PGE2 from human recombinant prostanoid EP4 receptor in CHEM1 cells after 2 hrs, IC50 = 0.0011 μM.	23403082
CHO	Function assay		30 mins	Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.0019 μM.	26985320
CHO	Function assay		30 mins	Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.0075 μM.	26985320
HEK293	Function assay		90 mins	Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay, EC50 = 0.346 μM.	26985320
CHO	Function assay		30 mins	Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.347 μM.	26985320
HEK293	Function assay		120 mins	Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method, IC50 = 0.00055 μM.	27876250
HEK293	Function assay		120 mins	Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method, IC50 = 0.0026 μM.	27876250
S2	Function assay			TP_TRANSPORTER: uptake in OAT4-expressing S2 cells, Km = 0.154 μM.	11907186
S2	Function assay			TP_TRANSPORTER: uptake in OCT2-expressing S2 cells, Km = 0.0289 μM.	11907186
HeLa	Function assay			TP_TRANSPORTER: uptake in PGT-expressing HeLa cells, K1/2 = 0.1 μM.	8787677
HeLa	Function assay			TP_TRANSPORTER: uptake in PGT-expressing HeLa cells, Km = 0.094 μM.	7754369
S2	Function assay			TP_TRANSPORTER: uptake in OAT3-expressing S2 cells, Km = 0.345 μM.	11907186
S2	Function assay			TP_TRANSPORTER: uptake in OCT1-expressing S2 cells, Km = 0.657 μM.	11907186
S2	Function assay			TP_TRANSPORTER: uptake in OAT2-expressing S2 cells, Km = 0.713 μM.	11907186
S2	Function assay			TP_TRANSPORTER: uptake in OAT1-expressing S2 cells, Km = 0.97 μM.	11907186
HEK293	Function assay			Inhibitory activity against human EP4 receptor expressed in HEK293 ebna cells, IC50 = 0.0007 μM.	12643927
HEK293	Function assay			EP4 agonist potency utilizing a stable clone of pSV40-EP4 transfected into HEK293 cells expressing EP4 receptor, EC50 = 0.003 μM.	12643927
Sf9	Function assay			TP_TRANSPORTER: uptake (vesicle) in membrane vesicles from MRP4-expressing Sf9 cells, Km = 3.4 μM.	12835412
HEK293	Function assay			Displacement of [3H]PGE2 from human EP3 receptor expressed in HEK293 cells, Ki = 0.00033 μM.	17531488
HEK293	Function assay			Displacement of [3H]PGE2 from human EP4 receptor expressed in HEK293 cells, Ki = 0.00079 μM.	17531488
HEK293	Function assay			Displacement of [3H]PGE2 from human EP2 receptor expressed in HEK293 cells, Ki = 0.0049 μM.	17531488
HEK293	Function assay			Displacement of [3H]PGE2 from human EP1 receptor expressed in HEK293 cells, Ki = 0.0091 μM.	17531488
HEK293	Function assay			Agonist activity against rat EP2 receptor expressed in HEK293 cells assessed as stimulation of cAMP release, EC50 = 0.0002 μM.	19250823
HEK293	Function assay			Agonist activity against rat EP4 receptor expressed in HEK293 cells assessed as stimulation of cAMP release, EC50 = 0.0007 μM.	19250823
HEK293	Function assay			Inhibition of rat EP4 receptor expressed in HEK293 cells, IC50 = 0.0021 μM.	19250823
HEK293	Function assay			Inhibition of rat EP2 receptor expressed in HEK293 cells, IC50 = 0.0052 μM.	19250823
CHO	Function assay			Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.0025 μM.	26985320
CHO	Function assay			Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.0037 μM.	26985320
Chem1	Function assay			Agonist activity at human FP receptor expressed in human Chem1 cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.25 μM.	26985320
HEK293	Function assay			Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells, IC50 = 0.0026 μM.	26988801
他の多くの細胞株試験データをご覧になる場合はこちらをクリックして下さい

生物活性

製品説明	Prostaglandin E2 (PGE2, Dinoprostone) plays important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts.

In Vitro
In vitro	PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain.^[1]
細胞実験	細胞株	Mice osteoclasts differentiated from bone marrow macrophages (BMMs)
	濃度	1, 10, 100, 1000, 10000 nM
	反応時間	18 h
	実験の流れ	When BMMs differentiated into mature osteoclasts, a TRAP staining kit is used to stain mature osteoclasts according to the manufacturer s instructions, and TRAP-positive osteoclasts with 5 or more nuclei are counted. For BMM migration, BMMs (1×10⁴ cells per well) are seeded in the upper chamber of the Transwell inserts with or without inhibitors and antagonists. PGE2 is added in lower chamber. The culture medium is the same between the upper chamber and lower chamber in the Transwell. After 18 h, the cells are fixed with 4% PFA and stained with crystal violet. The cells on the bottom of the Transwell insert are used to assess migration. At least five fields of view per insert are photographed, and migrated cells are counted.

In Vivo
In Vivo	PGE2 (0.3 μg/k, i.p.) significantly reduces the number of peritoneab macrophages undergoing phagocytosis of the methacrybate microbeads in rats.
動物実験	動物モデル	Male Sprague Dawley rats
	投与量	0.3 µg/kg
	投与経路	i.p.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試験 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06129604	Recruiting	Colorectal Carcinoma (CRC)\|Endometrial Carcinoma (EC)	University of Oklahoma	April 3 2024	Phase 2
NCT06307678	Completed	Periapical; Infection	Ataturk University	July 7 2023	Not Applicable
NCT06190132	Completed	Uremic Pruritus in Hemodialysis Patients	Ain Shams University	April 1 2023	Not Applicable

参考
[1] Fernandez-Repollet E, et al. J Histochem Cytochem. 1982 May;30(5):466-70.

参考

[1] Fernandez-Repollet E, et al. J Histochem Cytochem. 1982 May;30(5):466-70.

化学情報

分子量	352.47	化学式	C₂₀H₃₂O₅
CAS No.	363-24-6	SDF	Download Prostaglandin E2 (PGE2) SDFをダウンロードする
Smiles	CCCCCC(C=CC1C(CC(=O)C1CC=CCCCC(=O)O)O)O
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	1年-80°Cin solvent
保管	3年-20°C粉	溶液の保管冷凍と解凍の繰り返しを避けるため小分けにしてください	１ケ月-20°Cin solvent

In vitro
Batch:

DMSO : 70 mg/mL ( (198.59 mM)；吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Ethanol : 70 mg/mL

Water : 2.5 mg/mL

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量	濃度	体積	分子量
=	×	×

希釈計算器分子量計算器

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

投与量 mg/kg 動物平均体重 g 投与体積（動物毎）μL 動物数匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH₂O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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