Nivolumab (anti-PD-1)

製品コードA2002 別名: BMS-936558, ONO-4538, MDX-1106

製品コード：A2002 純度：99.87%

Nivolumab is a genetically engineered, fully human immunoglobulin (Ig) G4 monoclonal antibody directed against the negative immunoregulatory human cell surface receptor programmed death-1 (PD-1,PCD-1) with immune checkpoint inhibitory and antineoplastic activities. MW : 143.597 KD.

サイズ	価格(税別)	在庫状況
1mg	JPY 44500	国内在庫あり
1mg*5	JPY 146500	お問い合わせ
5mg	JPY 146500	国内在庫あり
1mg*25	JPY 445500	お問い合わせ

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（70）

質量管理及び製品安全説明書

現在のバッチを見る：純度： 99.87% タンパク質濃度： 8.8 mg/ml 内毒素： <1 EU/mg

99.87

PD-1/PD-L1阻害剤の選択性比較

生物活性

製品説明

同型

Human IgG4

Targets

PD-1/PD-L1 interaction ^[2] (Cell-free assay)	PD-1/PD-L2 interaction ^[2] (Cell-free assay)
2.52 nM	2.59 nM

In Vitro
In Vitro	The cell binding ratio of ¹²⁵I-Nivolumab and ¹²⁵I-Nivolumab-DTPA to PD-1 is 13.78 ± 2.90% and 10.22 ± 2.62%, respectively. Conjugating of DTPA does not affect targeting of Nivolumab to PD-1 in vitro.^[1]
細胞実験	Splenocytes were isolated from the spleens obtained from humanized PD-1 over-expressing mice following standard protocol. ¹²⁵I labeled Nivolumab and Nivolumab-DTPA, respectively. To perform co-culture, ¹²⁵I-Nivolumab and ¹²⁵I-Nivolumab-DTPA (0.37 MBq/µg) were added to the freshly isolated splenocytes (1×10⁶ cells) grown in 6-well plates, respectively. After incubation for 12 h at 4°C, cells were collected after rinsing three times with PBS. ¹²⁵I radioactivity was determined in a gamma counter to evaluate their binding efficiency to PD-1.

In Vivo
In Vivo	In SPECT images, lesions with high ^99mTc-DTPA-nivolumab uptake and relatively clear background are shown at 6 h. Thereafter, the suspected intestinal thickening in Gd-free T1WI is observed at 2 h after the addition of Gd-DTPA-nivolumab.^[1]
動物試験	Animal Models: CRC mouse models (highly expressed human PD-1 antigen) Dosages: 11.1 MBq/10 μg Administration: i.v. Reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883027/

参考
[1] Li D, et al. Front Bioeng Biotechnol. 2022 Feb 14;10:839756.

参考

[1] Li D, et al. Front Bioeng Biotechnol. 2022 Feb 14;10:839756.

製品説明

CAS No.	946414-94-4
Formulation	100 mM Pro-Ac, 20 mM Arg, pH5.0
Isotype	Human IgG4
Source	CHO cells
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Shipping	Shipped under low temperature conditions

各種クローンの論文数比較

^*Selleckは、市場の主要なクローンを収集し、2024年9月までの文献での使用状況を調査しました。その結果、Selleckの選定クローンが最も広く使用されていることが示されました。

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

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* 必須

よくある質問（FAQ）

質問：1：
How to store the antibody?

回答：
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.