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FCCP

別名：Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

FCCP化学構造

CAS No. 370-86-5

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代表番号: 045-509-1970|電子メール：[email protected]
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FCCP関連製品

OXPHOS阻害剤の選択性比較

Cell Data

Cell Lines Assay Type Concentration Incubation Time 活性情報 PMID
T47D Function assay 0.3 uM 15 mins Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay 20929261
T47D Function assay 3 uM 15 to 20 mins Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay 22938093
SH-SY5Y Function assay 10 uM 5 mins Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay 25265024
SH-SY5Y Function assay 10 uM 10 mins Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay 25265024
T47D Function assay 0.3 uM 3 to 12 mins Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay 26637046
T47D Function assay 0.3 uM 30 mins Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy 26637046
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method 28233680
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method 28233680
KOPN8 Function assay 10 uM 0.3 hrs Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis 31084028
T47D Function assay 1 to 10 uM Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA 20929261
MDA-MB-231 Cytotoxicity assay 1 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM 23245650
MDA-MB-231 Cytotoxicity assay 0.1 to 3 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor 23245650
MDA-MB-231 Function assay 0.3 uM Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM 23245650
T47D Function assay 0.1 to 3 uM Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM 22938093
T47D Function assay 10 to 30 uM Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin 22938093
T47D Function assay 0.3 to 1 uM Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin 22938093
Hep3B Function assay 10 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin 22938093
Hep3B Function assay 1 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin 22938093
TA3/Ha Function assay 6 uM Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis 24568614
T47D Function assay 0.3 uM Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate 26637046
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
HEK293 Function assay 1 to 3 uM Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay 31774672
T47D Function assay Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM 20929261
T47D Function assay Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM 20929261
HepG2 Function assay Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM ChEMBL
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生物活性

製品説明 FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
Targets
OXPHOS [2] ATP synthase [2]
In Vitro
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1].

FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].
細胞実験 細胞株 PC12 cells
濃度 30 μM
反応時間 30 min, 1h, 2h
実験の流れ

Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.
In Vivo
In Vivo

FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].
動物実験 動物モデル Female C57BL/6 mice
投与量 1 mg/kg
投与経路 i.p.

化学情報

分子量 254.17 化学式

C10H5F3N4O
CAS No. 370-86-5 SDF Download FCCP SDFをダウンロードする
Smiles C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F
保管

In vitro
Batch:

DMSO : 6 mg/mL ( (23.6 mM)； 吸湿したDMSOは溶解度を減少させます。新しいDMSOをご使用ください。)

Water : Insoluble

Ethanol : Insoluble

モル濃度計算器

in vivo
Batch:

Add solvents to the product individually and in order.

投与溶液組成計算機

実験計算

モル濃度計算器

質量 濃度 体積 分子量

投与溶液組成計算機(クリア溶液)

ステップ１：実験データを入力してください。（実験操作によるロスを考慮し、動物数を1匹分多くして計算・調製することを推奨します）

mg/kg g μL 匹

ステップ２：投与溶媒の組成を入力してください。（ロット毎に適した溶解組成が異なる場合があります。詳細については弊社までお問い合わせください）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

投与溶媒濃度： mg/ml;

DMSOストック溶液調製方法： mg 試薬を μL DMSOに溶解する（濃度 mg/mL, 注：濃度が当該ロットのDMSO溶解度を超える場合はご連絡ください。 )

投与溶媒調製方法：Take μL DMSOストック溶液に μL PEG300，を加え、完全溶解後μL Tween 80，を加えて完全溶解させた後 μL ddH2O，を加え完全に溶解させます。

投与溶媒調製方法：μL DMSOストック溶液に μL Corn oil，を加え、完全溶解。

注意：１.ストック溶液に沈殿、混濁などがないことをご確認ください；
２.順番通りに溶剤を加えてください。次のステップに進む前に溶液に沈殿、混濁などがないことを確認してから加えてください。ボルテックス、ソニケーション、水浴加熱など物理的な方法で溶解を早めることは可能です。

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