Durvalumab (anti-PD-L1)

製品コードA2013 別名: MEDI4736

製品コード：A2013 純度：99.68%

Durvalumab is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.

サイズ	価格(税別)	在庫状況
1mg	JPY 44500	国内在庫あり
1mg*5	JPY 146500	お問い合わせ
5mg	JPY 146500	国内在庫あり
1mg*25	JPY 445500	お問い合わせ

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（13）

カスタマーフィードバック1例

質量管理及び製品安全説明書

現在のバッチを見る：純度： 99.68% タンパク質濃度： 5 mg/ml 内毒素： <1 EU/mg

99.68

PD-1/PD-L1阻害剤の選択性比較

生物活性

製品説明

Durvalumab is a selective, high affinity human IgG1 mAb that blocks programmed cell death ligand-1 (PD-L1) binding to PD-1 (IC50=0.1 nM) and CD80 (IC50=0.04 nM). MW=146.3 kDa.

同型

Human IgG1

Targets

PD-L1/CD80 ^[1] (HTRF assay)	PD-1/PD-L1 interaction ^[1] (HTRF assay)
0.04 nM	0.1 nM

In Vitro
In Vitro	The labeled radioligand shows good affinity to high PD-L1 expression cells and could be blocked with excess unlabeled intact durvalumab.^[1]
細胞実験	The A549 and H460 cells were cultured in six-well plates (~106 cells/well) and divided into two groups. All of the wells were incubated with 5% BSA for blocking nonspecific binding. Group A was presaturated with 100-fold excess of unlabeled durvalumab to block the binding of ¹²⁴I-Durva-F(ab′)₂, and group B had only the medium added. After 30 min, 2 nM of ¹²⁴I-Durva-F(ab′)₂ was added to each group and incubated for 1 h at 37 °C in a cell incubator. After supernatant collection, the cells were lysed using 2 M of NaOH.

In Vivo
In Vivo	The peak tumor uptake of ¹²⁴I-Durva-F(ab’)₂ was close to ¹²⁴I-Durva, but much earlier (5.29±0.42% ID/g for 124I-Durva-F(ab’)₂ at 12 h vs 5.18±0.73% ID/g for ¹²⁴I-Durva at 48 h). Compared with ¹²⁴I-Durva, an accelerated blood clearance was observed for ¹²⁴I-Durva-F(ab’)₂, allowing for a higher tumor-to-background ratio.^[1]
動物試験	Animal Models: H460 and A549 tumor-bearing mice Dosages: 20 μCi Administration: i.v.

参考
[1] Cheng Y, et al. Mol Pharm. 2022 Mar 4.

参考

[1] Cheng Y, et al. Mol Pharm. 2022 Mar 4.

製品説明

CAS No.	1428935-60-7
Formulation	100 mM Pro-Ac, 20 mM Arg, pH 5.0
Isotype	Human IgG1
Source	CHO cells
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Shipping	Shipped under low temperature conditions

各種クローンの論文数比較

^*Selleckは、市場の主要なクローンを収集し、2024年9月までの文献での使用状況を調査しました。その結果、Selleckの選定クローンが最も広く使用されていることが示されました。

技術サポート

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Handling Instructions

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* 必須

よくある質問（FAQ）

質問：1：
How to store the antibody?

回答：
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.