Cemiplimab (anti-PD-1)

製品コードA2022 別名: REGN-2810, cemiplimab-rwlc

製品コード：A2022 純度：99.3%

Cemiplimab (anti-PD-1) is an intravenous human monoclonal antibody directed against programmed cell death-1 receptor (PD-1) and blocks its interaction with programmed death ligands 1 (PD-L1) and 2 (PD-L2). Cemiplimab blocks T-cell inactivation and enhances the immune system's anti-tumor response.

サイズ	価格(税別)	在庫状況
1mg	JPY 44500	国内在庫あり
1mg*5	JPY 146500	お問い合わせ
1mg*25	JPY 445500	お問い合わせ

代表番号: 045-509-1970\|電子メール：[email protected] よく尋ねられる質問

文献中Selleckの製品使用例（1）

質量管理及び製品安全説明書

現在のバッチを見る：純度： 99.3% タンパク質濃度： 5 mg/ml 内毒素： <1 EU/mg

99.3

PD-1/PD-L1阻害剤の選択性比較

生物活性

製品説明	Cemiplimab (anti-PD-1) is an intravenous human monoclonal antibody directed against programmed cell death-1 receptor (PD-1) and blocks its interaction with programmed death ligands 1 (PD-L1) and 2 (PD-L2). Cemiplimab blocks T-cell inactivation and enhances the immune system's anti-tumor response.
同型	Human IgG4

In Vitro
In Vitro	In cell-based assays, REGN2810 reverses PD-1-dependent attenuation of T-cell receptor signaling in engineered T cells and enhances responses of human primary T cells.^[1]
細胞実験	Objective: T cell activation bioassays Cells: Jurkat/AP-1-Luc/hPD-1 cells, HEK293/hCD20 cells, HEK293/hCD20/hPD-L1 cells Concentrations: 0.1 pM-0.1 μM Incubation Time: 4-6 h Method: Antigenpresenting cell (APC)-like HEK293 cells are generated by lentiviral transduction of human CD20 and human PD-L1. T-cell receptor (TCR) activation is achieved by an antiCD3 x anti-CD20 bispecific antibody. To generate a dose response curve for anti-CD3 x anti-CD20, the bispecific molecule is serially diluted and tested with 50,000/well Jurkat/AP-1-Luc/hPD-1 and 10,000/well HEK293/hCD20 or HEK293/hCD20/hPD-L1 cells in a 96-well plate. Serially diluted REGN2810 is tested under similar conditions in the presence of a fixed concentration of anti-CD3 x anti-CD20 (100 pM). Plates are incubated at 37°C for 4-6 hours. Reference: https://pubmed.ncbi.nlm.nih.gov/28265006/ Objective: T cell activation bioassays Cells: HEK293/hCD20/hPD-L1 cells, CD4+ T cells, HEK293/hCD20/hPD-L1 cells Concentrations: 0.1 pM-0.1 μM Incubation Time: 72 h Method: HEK293/hCD20/hPD-L1 cells are treated with 50 mg/mL mitomycin C for 30 minutes at 37°C to inhibit proliferation. Serially diluted REGN2810 is incubated with 50,000/well preactivated CD4+ T cells and 25,000/well of HEK293/hCD20/hPD-L1 cells in the presence of 2 nM anti-CD3 x anti-CD20 bispecific antibody in a 96-well plate for 72 hours. Reference: https://pubmed.ncbi.nlm.nih.gov/28265006/ Cemiplimab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

In Vivo
In Vivo	Cemiplimab (REGN2810) binds the humanized PD-1 receptor and inhibits growth of MC38 murine tumors in human PD-1 knock-in mice. As REGN2810 binds to cynomolgus monkey PD-1 with high affinity, pharmacokinetic and toxicologic assessment of REGN2810 is performed in cynomolgus monkeys. High doses of REGN2810 are well tolerated, without adverse immune-related effects.^[1]
動物試験	Objective: MC38 mouse colon carcinoma Animal Models: adult human PD-1 knock-in mice Formulation: -- Dosages: 0.3 mg/kg, 1 mg/kg, 3 mg/kg, 10 mg/kg Administration: i.p. Reference: https://pubmed.ncbi.nlm.nih.gov/28265006 Objective: pharmacokinetic (PK) study Animal Models: cynomolgus monkeys (Macaca fascicularis) Formulation: -- Dosages: 1 mg/kg, 5 mg/kg, 15 mg/kg Administration: i.v. Reference: https://pubmed.ncbi.nlm.nih.gov/28265006 Cemiplimab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

参考
[1] Elena Burova, et al. Mol Cancer Ther. 2017 May;16(5):861-870.

参考

[1] Elena Burova, et al. Mol Cancer Ther. 2017 May;16(5):861-870.

製品説明

CAS No.	1801342-60-8
Formulation	PBS buffer. Contains no stabilizers or preservatives
Isotype	Human IgG4
Source	HEK293
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Shipping	Shipped under low temperature conditions

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