ホームページ 一次抗体 NDRG2 Rabbit mAb For research use only.
Not for use in humans.

NDRG2 Rabbit mAb

Catalog No.: F3171

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使用情報

Dilution
1:1000
1:20
1:100
Application
WB, IP, IHC
Source
Rabbit
Reactivity
Human, Mouse, Rat
Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years
Predicted MW Observed MW
40 kDa 41 kDa, 40 kDa
*なぜ予測分子量と実際の分子量が異なるのか？
下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

プロトコール

IHC
Experimental Protocol：
 
Deparaffinization/Rehydration
1. Deparaffinize/hydrate sections:
2. Incubate sections in three washes of xylene for 5 min each.
3. Incubate sections in two washes of 100% ethanol for 10 min each.
4. Incubate sections in two washes of 95% ethanol for 10 min each.
5. Wash sections two times in dH2O for 5 min each.
6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
 
Staining
1. Wash sections in dH2O three times for 5 min each.
2. Incubate sections in 3% hydrogen peroxide for 10 min.
3. Wash sections in dH2O two times for 5 min each.
4. Wash sections in wash buffer for 5 min.
5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature.
6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C.
7. Remove antibody solution and wash sections with wash buffer three times for 5 min each.
8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature.
9. Wash sections three times with wash buffer for 5 min each.
10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use.
11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.
12. Immerse slides in dH2O.
13. If desired, counterstain sections with hematoxylin.
14. Wash sections in dH2O two times for 5 min each.
15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each.
16. Mount sections with coverslips and mounting medium.
 

Datasheet & SDS

生物学的記述

Specificity
NDRG2 Rabbit mAb recognizes endogenous levels of total NDRG2 protein. This antibody does not cross-react with human NDRG4. This antibody has not been validated for IHC in Mouse and Rat.
Clone
J2P5
Background
N-myc downstream-regulated gene 2 (NDRG2) is a cytoplasmic protein highly expressed in the brain, kidney, skeletal muscle, and other tissues, playing a crucial role in cellular stress response, metabolism, and tumor suppression. It regulates neuroprotection, astrocyte activation, and synaptic plasticity, with implications for psychiatric disorders like depression and ADHD. As a tumor suppressor, NDRG2 inhibits proliferation, invasion, and epithelial-mesenchymal transition (EMT) by modulating key pathways such as PI3K/Akt, Wnt/β-catenin, and TGF-β/Smad. It also influences metabolic homeostasis by regulating glucose metabolism, AMPK signalling, and oxidative stress responses. Additionally, NDRG2 stabilizes the Na⁺/K⁺-ATPase α1-subunit, enhancing Na⁺ transport and ion homeostasis, with its expression being modulated by steroid hormones like estrogen, linking it to kidney function and sodium balance. Through interactions with signalling molecules such as PTEN, GSK-3β, and NF-κB, NDRG2 impacts transcriptional regulation, cell cycle control, and autophagy.
References

技術サポート

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