MAD2L1 Rabbit mAb

Catalog No.: F3011

当該製品は品切れ状态で、ごメールアドレスを教えていただければ、在庫があると、メールで顧客様に伝えます。

您「在庫のお知らせ」をクリックして、ごメールアドレスを入力していただくと、10％の割引を取得することができます。

使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
22 kDa 22 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
MAD2L1 Rabbit mAb recognizes endogenous levels of total MAD2L1 protein.

Synonym(s)
MAD2,MAD2L1

Clone
P22B18

Background
MAD2L1 (Mad2) is a crucial component of the spindle assembly checkpoint (SAC), a mechanism that ensures accurate chromosome segregation during mitosis by preventing premature anaphase onset. It exists in two conformational states: the open, inactive O-MAD2 and the closed, active C-MAD2. During mitosis, unattached kinetochores convert O-MAD2 into C-MAD2, which binds to and inhibits Cdc20, a coactivator of the anaphase-promoting complex/cyclosome (APC/C). This inhibition delays anaphase until all chromosomes are properly attached to the spindle, ensuring genomic stability. MAD2L1’s conformational switch and interactions with proteins like BubR1, Bub3, and Cdc20 are essential for SAC function. It also plays roles in DNA repair, apoptosis, and stress responses. Dysregulation of MAD2L1, through mutations or overexpression, leads to chromosomal instability and aneuploidy, contributing to cancers such as breast and ovarian cancer.

Background

MAD2L1 (Mad2) is a crucial component of the spindle assembly checkpoint (SAC), a mechanism that ensures accurate chromosome segregation during mitosis by preventing premature anaphase onset. It exists in two conformational states: the open, inactive O-MAD2 and the closed, active C-MAD2. During mitosis, unattached kinetochores convert O-MAD2 into C-MAD2, which binds to and inhibits Cdc20, a coactivator of the anaphase-promoting complex/cyclosome (APC/C). This inhibition delays anaphase until all chromosomes are properly attached to the spindle, ensuring genomic stability. MAD2L1’s conformational switch and interactions with proteins like BubR1, Bub3, and Cdc20 are essential for SAC function. It also plays roles in DNA repair, apoptosis, and stress responses. Dysregulation of MAD2L1, through mutations or overexpression, leads to chromosomal instability and aneuploidy, contributing to cancers such as breast and ovarian cancer.

References
[1] Skinner JJ, et al. J Cell Biol. 2008 Dec 1;183(5):761-8.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

技術サポート

ストックの作り方、阻害剤の保管方法、細胞実験や動物実験の際に注意すべき点など、製品を取扱う時に問い合わせが多かった質問に対しては取扱説明書でお答えしています。

Handling Instructions

他に質問がある場合は、お気軽にお問い合わせください。

* 必須

* 大学・企業名 大学・企業名を記入してください

* 名前 名前を記入してください

* 電子メール 電子メール・アドレスを記入してください 有効なメールアドレスを入力してください

電話番号

* 内容 お問い合わせ内容をご入力ください

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%