IRP1 Rabbit mAb

Catalog No.: F3065

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使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit

Reactivity
Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
98 kDa 98 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
IRP1 Rabbit mAb recognizes endogenous levels of total IRP1 protein.

Clone
M7K13

Background
Iron Regulatory Protein 1 (IRP1) is a dual-function protein that plays a central role in maintaining cellular iron homeostasis. In iron-replete conditions, IRP1 binds a 4Fe-4S cluster, functioning as a cytosolic aconitase that catalyzes the isomerization of citrate to isocitrate. Under iron-deficient conditions, IRP1 loses its Fe-S cluster and transforms into an RNA-binding protein, binding to iron-responsive elements (IREs) in the untranslated regions (UTRs) of mRNAs involved in iron metabolism. By binding to 5' UTR IREs, IRP1 represses translation, while binding to 3' UTR IREs stabilizes mRNA, thereby modulating iron uptake, storage, and utilization. IRP1 undergoes conformational changes to accommodate IRE binding, with its structure transitioning from a closed, compact form in the aconitase state to an open, L-shaped conformation in the RNA-bound state. This functional switch is regulated by iron availability, oxidative stress, phosphorylation, and reactive oxygen and nitrogen species. Dysregulation of IRP1 is linked to disorders of iron metabolism.

Background

Iron Regulatory Protein 1 (IRP1) is a dual-function protein that plays a central role in maintaining cellular iron homeostasis. In iron-replete conditions, IRP1 binds a 4Fe-4S cluster, functioning as a cytosolic aconitase that catalyzes the isomerization of citrate to isocitrate. Under iron-deficient conditions, IRP1 loses its Fe-S cluster and transforms into an RNA-binding protein, binding to iron-responsive elements (IREs) in the untranslated regions (UTRs) of mRNAs involved in iron metabolism. By binding to 5' UTR IREs, IRP1 represses translation, while binding to 3' UTR IREs stabilizes mRNA, thereby modulating iron uptake, storage, and utilization. IRP1 undergoes conformational changes to accommodate IRE binding, with its structure transitioning from a closed, compact form in the aconitase state to an open, L-shaped conformation in the RNA-bound state. This functional switch is regulated by iron availability, oxidative stress, phosphorylation, and reactive oxygen and nitrogen species. Dysregulation of IRP1 is linked to disorders of iron metabolism.

References
[1] Volz K. Curr Opin Struct Biol. 2008 Feb;18(1):106-11.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%