GPCR RDC1/CXCR-7 Rabbit mAb

Catalog No.: F2937

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使用情報

Dilution
WB1:1000

Application
WB

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
41 kDa 41 kDa
^*なぜ予測分子量と実際の分子量が異なるのか？下記の原因により、実際の分子量が予測と異なる：タンパク質の翻訳後修飾（リン酸化/糖鎖付加），スプライシングバリアント，イソフォーム，相対的な電荷，ポリマー。

Datasheet & SDS

生物学的記述

Specificity
GPCR RDC1/CXCR-7 Rabbit mAb recognizes endogenous levels of total gpcr rdc1/cxcr-7 protein.

Synonym(s)
CXCR7,GPCR RDC1

Clone
G15F11

Background
CXCR7, also known as RDC1, is an atypical G protein-coupled receptor (GPCR) that binds chemokines CXCL12 (SDF-1) and CXCL11 (I-TAC). Unlike typical GPCRs, CXCR7 does not activate classical G protein signalling pathways but instead functions as a scavenger receptor, internalizing and degrading CXCL12 through β-arrestin-mediated endocytosis. This unique mechanism regulates chemokine gradients, critical for processes like cell migration, tissue development, and immune responses. By controlling CXCL12 availability, CXCR7 modulates the activity of CXCR4, the canonical receptor for CXCL12, fine-tuning its signalling and functional responses. CXCR7 plays an essential role in embryonic development, particularly in heart morphogenesis, where it regulates semilunar valve formation and vascular remodelling. It is also crucial for immune cell trafficking, influencing the migration of B cells, T cells, and other leukocytes by shaping chemokine gradients in lymphoid organs and inflammatory sites. In cancer, CXCR7 promotes tumor progression, metastasis, and angiogenesis by altering the tumor microenvironment and enhancing the CXCR4-mediated migration of cancer cells. It also forms heterodimers with CXCR4, further modulating its signalling and impacting cell survival, proliferation, and chemotaxis. The receptor’s involvement in development and disease is mediated through its regulation of key signalling pathways, such as BMP (bone morphogenetic protein) and EGFR (epidermal growth factor receptor), which influence cell proliferation and tissue patterning. Dysregulation of CXCR7 is linked to various pathologies, including cancer, congenital heart defects, and inflammatory disorders.

Background

CXCR7, also known as RDC1, is an atypical G protein-coupled receptor (GPCR) that binds chemokines CXCL12 (SDF-1) and CXCL11 (I-TAC). Unlike typical GPCRs, CXCR7 does not activate classical G protein signalling pathways but instead functions as a scavenger receptor, internalizing and degrading CXCL12 through β-arrestin-mediated endocytosis. This unique mechanism regulates chemokine gradients, critical for processes like cell migration, tissue development, and immune responses. By controlling CXCL12 availability, CXCR7 modulates the activity of CXCR4, the canonical receptor for CXCL12, fine-tuning its signalling and functional responses. CXCR7 plays an essential role in embryonic development, particularly in heart morphogenesis, where it regulates semilunar valve formation and vascular remodelling. It is also crucial for immune cell trafficking, influencing the migration of B cells, T cells, and other leukocytes by shaping chemokine gradients in lymphoid organs and inflammatory sites. In cancer, CXCR7 promotes tumor progression, metastasis, and angiogenesis by altering the tumor microenvironment and enhancing the CXCR4-mediated migration of cancer cells. It also forms heterodimers with CXCR4, further modulating its signalling and impacting cell survival, proliferation, and chemotaxis. The receptor’s involvement in development and disease is mediated through its regulation of key signalling pathways, such as BMP (bone morphogenetic protein) and EGFR (epidermal growth factor receptor), which influence cell proliferation and tissue patterning. Dysregulation of CXCR7 is linked to various pathologies, including cancer, congenital heart defects, and inflammatory disorders.

References
[1] Yu S, et al. Dev Dyn. 2011 Feb;240(2):384-93. [2] Levoye A, et al. Blood. 2009 Jun 11;113(24):6085-93.

PVDFメンブレン孔径参考表

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%