Yoda1

製品コードS6678 バッチS667804

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₃H₈Cl₂N₄S₂
	分子量	355.27	CAS No.	448947-81-7
Solubility (25°C)*	体外	DMSO	23 mg/mL (64.73 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Yoda1 acts as an agonist for both human and mouse Piezo1(EC50 of 17.1 and 26.6 μM for mouse and human). Yoda1 activates purified Piezo1 channels in the absence of other cellular components.
in vitro	Yoda-1 dramatically increases the YAP expression and ECM secretion reduced by Piezo1 silencing in vitro.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Immortalized mouse renal MCs (SV40-MES-13)
	濃度	--
	反応時間	48 h
	実験の流れ	Immortalized mouse renal MCs (SV40-MES-13) were cultured in DMEM/F12 containing 5% fetal bovine serum (FBS), 0.5% penicillin and streptomycin in an atmosphere of 5% CO₂ at 37 ℃. Following overnight serum deprivation, the SV40-MES-13 cells are seeded on cover slips coated with either 2 kPa (soft) or 50 kPa (stiff) hydrogels, and then treated with the YAP inhibitor Super-TDU, Piezo1 activator Yoda-1, MEK1/2 inhibitor PD0325901, JNK inhibitor SP600125, or the p38 kinase inhibitor SB203580 for 48 h.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Ion channel Piezo1 activation aggravates the endothelial dysfunction under a high glucose environment [ Cardiovasc Diabetol, 2024, 23(1):150]	PubMed: 38702777
Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406]	PubMed: 38694622
Low-intensity pulsed ultrasound promotes the osteogenesis of mechanical force-treated periodontal ligament cells via Piezo1 [ Front Bioeng Biotechnol, 2024, 12:1347406]	PubMed: 38694622
Activation of Piezo1 increases the sensitivity of breast cancer to hyperthermia therapy [ Open Med (Wars), 2024, 19(1):20240898]	PubMed: 38463518
Prolonged Piezo1 Activation Induces Cardiac Arrhythmia [ Int J Mol Sci, 2023, 24(7)6720]	PubMed: 37047693
Prolonged Piezo1 Activation Induces Cardiac Arrhythmia [ Int J Mol Sci, 2023, 24(7)6720]	PubMed: 37047693
Inhibition of Piezo1 ameliorates intestinal inflammation and limits the activation of group 3 innate lymphoid cells in experimental colitis [ J Innate Immun, 2023, 15(1):709-23]	PubMed: 37725937
Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis [ Sci Adv, 2023, 9(22):eadg0478]	PubMed: 37267365
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4): 1621-1638]	PubMed: N/A
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4):1621-1638]	PubMed: 35198061

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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