Vincristine

製品コードS9555

印刷

化学情報

Chemical Structure Synonyms Leurocristine, NSC-67574, 22-Oxovincaleukoblastine Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C46H56N4O10
分子量 824.96 CAS No. 57-22-7
Solubility (25°C)* 体外 DMSO 100 mg/mL (121.21 mM)
Ethanol 100 mg/mL (121.21 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Vincristine (Leurocristine, NSC-67574, 22-Oxovincaleukoblastine) is a natural alkaloid isolated from the plant Vinca rosea Linn. with anti-tumor activity. Vincristine inhibits microtubule formation in mitotic spindle and binds to microtubule with Ki of 85 nM.
in vitro

Vincristine, a microtubule-destabilizing agent (MDA), inhibits the formation of microtubules and mitosis of the cancer cell, which is a victim of intratumoural metabolic resistance that has no important relevance with neither CYP3A4 nor CYP3A5.[2]
in vivo

Vincristine shows the regression of the tumor in zebrafish xenografts.[3]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HepG2_EV, HepG2_CYP3A4, HepG_CYP3A5, HepG2_CYP2C8 cells
濃度 1 μM
反応時間 24, 48 and 72 h
実験の流れ

HepG2 sublines (100×104 cells/well) are seeded in 12-well plates and cultured until full confluence. Then, the media is removed, and the cells are exposed to 1 μM drug (docetaxel, paclitaxel or vincristine) or 0.01% DMSO (vehicle control). Samples are collected at 24, 48 and 72 h intervals using TRI Reagent, which is followed by RNA isolation. The RNA yield is assessed via a NanoDrop ND-1000 spectrophotometer, while its quality and integrity are verified by agarose gel electrophoresis. 
動物実験 動物モデル Zebrafish of glioma model
投薬量 10-20 nM
投与方法 p.o.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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