Trolox

製品コードS3665 バッチS366505

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₄H₁₈O₄
	分子量	250.29	CAS No.	53188-07-1
Solubility (25°C)*	体外	DMSO	50 mg/mL (199.76 mM)
		Ethanol	25 mg/mL (99.88 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.
in vitro	Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid) is a vitamin E analog and a direct scavenger of peroxyl and alkoxyl radicals. Trolox impairs PT4 cell survival, has minor effects on ROS levels and causes wide deregulation of cell cycle genes^[1]. Trolox enhances As2O3-mediated apoptosis in APL, myeloma, and breast cancer cells. Trolox inhibits DNA damage formation induced by singlet oxygen in human lymphoblast WTK-1 cells and protects red blood cells during photodynamic treatment^[2].
in vivo	After cerebral ischemia reperfusion, Trolox prescription increases anti-apoptotic proteins and decreased proapoptotic proteins thus protects neurons of the hippocampus and caused improvement of memory^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	NB4, IM9, and AsR2 cells
	濃度	100 μM
	反応時間	6 days
	実験の流れ	NB4, IM9, and AsR2 cells are seeded at 1 × 10⁵ cells/mL in 24-well plates. Cells are treated with various concentrations of As2O3 or doxorubicin, alone or in combination with 100 μM trolox for 6 days. Viable cells are counted by trypan blue exclusion on days 1, 3, and 6. All cells are maintained at a density lower than 1 × 10⁶ cells/mL through dilution as required, and media ± treatment is replaced every third day. MCF-7, MDA-231, and T47D are seeded in 24-well plates at a density of 4000 cells/well. The next day, fresh media containing As2O3 ± trolox is added. On the days indicated, cells are fixed in 10% trichloroacetic acid and subsequently stained with sulforhodamine B (SRB). Bound SRB is solubilized in 10 mM unbuffered Tris (tris(hydroxymethyl)aminomethane), and optical density is measured at 570 nm in a microplate reader.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Strong Protection by 4-Hydroxyestrone against Erastin-Induced Ferroptotic Cell Death in Estrogen Receptor-Negative Human Breast Cancer Cells: Evidence for Protein Disulfide Isomerase as a Mechanistic Target for Protection [ Biochemistry, 2024, 63(8):984-999]	PubMed: 38569593
EP1 activation inhibits doxorubicin-cardiomyocyte ferroptosis via Nrf2 [ Redox Biol, 2023, 65:102825]	PubMed: 37531930
Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancer [ Cell Death Dis, 2023, 14(8):515]	PubMed: 37573361
Co-inhibition of glutaminolysis and one-carbon metabolism promotes ROS accumulation leading to enhancement of chemotherapeutic efficacy in anaplastic thyroid cancer [ Cell Death Dis, 2023, 14(8):515]	PubMed: 37573361
Decreased IDO1-dependent tryptophan metabolism in aged lung during influenza [ Eur Respir J, 2021, 57(5)2000443]	PubMed: 33243840
pH-dependent rearrangement determines the iron-activation and antitumor activity of artemisinins [ Free Radic Biol Med, 2021, 163:234-242]	PubMed: 33359684
Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza [ Eur Respir J, 2020, 2000443]	PubMed: 33243840
Hyperglycemia and Advanced Glycation End Products Disrupt BBB and Promote Occludin and claudin-5 Protein Secretion on Extracellular Microvesicles [ Sci Rep, 2020, 29;10(1):7274]	PubMed: 32350344

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。