Sulforaphane

製品コードS5771 バッチS577104

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 2 years -20°C(in the dark) liquid
化学式

C6H11NOS2
分子量 177.29 CAS No. 4478-93-7
Solubility (25°C)* 体外  
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

生物活性

製品説明 Sulforaphane is a naturally occurring isothiocyanate derived from the consumption of cruciferous vegetables, such as broccoli, cabbage, and kale. It is an inducer of Nrf2. Sulforaphane is also an inhibitor of histone deacetylase (HDAC) and NF-κB. Sulforaphane increases heme oxygenase-1 (HO-1) and reduces the levels of reactive oxygen species (ROS). Sulforaphane induces cell cycle arrest and apoptosis.
in vitro

Sulforaphane, reactive oxygen species (ROS) inhibitor, decrease the autophagy induced by CC90003, while cell death strengthens, confirming that CC90003 induces autophagy by activating ROS/p53.[3]
in vivo

Natural isothiocyanate and potent NRF2 activator sulforaphane reverses diet-induced obesity through a predominantly, but not exclusively, NRF2-dependent mechanism that requires a functional leptin receptor signaling and hyperleptinemia.[4]
密度 1.17 g/mL

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human CRC cell HCT-116 and SW620
濃度 20 μM
反応時間 24 h
実験の流れ

Cells are cultured in a 96-well microplate at a density of 5×103 cells/well for 24 h. Then, the cells are divided into several groups and treated with different conditions. Cell viability is assessed with CCK-8 after treating drugs. The absorbance value at 450 nm is read with a 96-well plate reader. Nuclear staining with Hoechst33342 is used to analyze the apoptosis. For colony formation assays, an equal number of cells after different treatments are planted in six-well plates.Colonies are visualized by crystal violet staining 14 days after plating.
動物実験 動物モデル diet-induced obese mice
投薬量 25 μl
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Engineered Cas9 variants bypass Keap1-mediated degradation in human cells and enhance epigenome editing efficiency [ Nucleic Acids Res, 2024, gkae761] PubMed: 39228373
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
A novel effect of sulforaphane on promoting mouse granulosa cells proliferation via the NRF2-TKT pathway [ J Adv Res, 2024, S2090-1232(24)00422-3] PubMed: 39341455
Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Druggable redox pathways against Mycobacterium abscessus in cystic fibrosis patient-derived airway organoids [ PLoS Pathog, 2023, 19(8):e1011559] PubMed: 37619220
Identifying autophagy-related genes as potential targets for immunotherapy in tuberculosis [ Int Immunopharmacol, 2023, 118:109956] PubMed: 36931175
Variable p53/Nrf2 crosstalk contributes to triptolide-induced hepatotoxic process [ Toxicol Lett, 2023, 379:67-75] PubMed: 36990132
SARS-CoV-2 virus NSP14 Impairs NRF2/HMOX1 activation by targeting Sirtuin 1 [ Cell Mol Immunol, 2022, 19(8):872-882] PubMed: 35732914
Sulforaphane reduces obesity by reversing leptin resistance [ Elife, 2022, 11e67368] PubMed: 35323110
Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages [ Int J Mol Sci, 2022, 23(15)8603] PubMed: 35955737

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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