SAG (Smoothened Agonist)

製品コードS6384 バッチS638403

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₂₈H₂₈ClN₃OS

分子量

490.06

CAS No.

912545-86-9

Solubility (25°C)*

体外

DMSO

98 mg/mL (199.97 mM)

Ethanol

98 mg/mL (199.97 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	4.9mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 98 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	0.8mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SAG (Smoothened Agonist) is a potent agonist of Smoothened (Smo). Smoothened Agonist induces firefly luciferase expression in Shh-LIGHT2 cells with an EC50 of 3 nM.
in vitro	SAG can counteract glucocorticoids (GCs) antagonism of Shh-driven granule neuron precursor (CGNP) proliferation.^[2]
in vivo	Systemic administration of Smoothened Agonist (SAG) prevents the neurotoxic effects of glucocorticoids. Smoothened Agonist can cross the blood-brain barrier to activate Shh transcriptional targets in vivo. SAG at the treatment dose effectively prevents GC-induced neonatal cerebellar developmental abnormalities.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Granule neuron precursor (CGNP) primary cells
	濃度	15-240 nM
	反応時間	24 h
	実験の流れ	CGNP are stimulated by various SAG concentrations (15 to 240 nM) compared with ShhN (3 μg/ml) and vehicle after 24 hours in vitro. Quantitative reverse transcription–PCR is performed with SYBR Green master mix in LightCycler 480.
動物実験	動物モデル	Ptc^+/- mouse medulloblastoma model, Swiss Webster B pups
	投薬量	20 mg/kg, 140 mg/kg
	投与方法	i.p.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Identification of a distal enhancer regulating hedgehog interacting protein gene in human lung epithelial cells [ EBioMedicine, 2024, 101:105026]	PubMed: 38417378
Sonic hedgehog restrains the ubiquitin-dependent degradation of SP1 to inhibit neuronal/glial senescence associated phenotypes in chemotherapy-induced peripheral neuropathy via the TRIM25-CXCL13 axis [ J Adv Res, 2024, S2090-1232(24)00106-1]	PubMed: 38479571
Defining cis-regulatory elements and transcription factors that control human cortical interneuron development [ iScience, 2024, 27(6):109967]	PubMed: 38827400
Single-cell RNA sequencing reveals the developmental program underlying proximal-distal patterning of the human lung at the embryonic stage [ Cell Res, 2023, 33(6):421-433]	PubMed: 37085732
Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly [ Biofabrication, 2023, 15(4).]	PubMed: 37552975
Triptolide inhibits epithelial ovarian tumor growth by blocking the hedgehog/Gli pathway [ Aging (Albany NY), 2023, 10.18632/aging.205110]	PubMed: 37851362
Primary cilia support cartilage regeneration after injury [ Int J Oral Sci, 2023, 15(1):22]	PubMed: 37268650
Protocol for the rapid intravenous in ovo injection of developing amniote embryos [ STAR Protoc, 2023, 4(2):102324]	PubMed: 37210721
Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content [ Nat Commun, 2022, 13(1):2387]	PubMed: 35501322
The PROTAC selectively degrading Bcl-xL represents a novel Hedgehog pathway inhibitor with capacity of combating resistance to Smoothened inhibitors while sparing bone growth [ Theranostics, 2022, 12(17):7476-7490]	PubMed: 36438482

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。