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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
化学式 | C17H17ClN2O5 |
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分子量 | 364.78 | CAS No. | 305834-79-1 | |
Solubility (25°C)* | 体外 | DMSO | 73 mg/mL (200.12 mM) | |
Ethanol | 36 mg/mL warmed with 50ºC water bath (98.68 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation. |
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in vitro | SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2] |
in vivo | In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1] |
キナーゼアッセイ | Cytosolic phosphorylation of Akt | |
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Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting. | ||
細胞アッセイ | 細胞株 | HsSultan and NB4 cells |
濃度 | 8 μg/mL | |
反応時間 | 24 h | |
実験の流れ | HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm. |
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動物実験 | 動物モデル | Permanent focal cerebral ischemia mouse model |
投薬量 | 0.04 mg/g | |
投与方法 | i.p. |
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Data from [Data independently produced by , , J Exp Clin Cancer Res, 2018, 37(1):41]
Data from [Data independently produced by , , J Exp Clin Cancer Res, 2017, 36(1):105]
Data from [Data independently produced by , , EBioMedicine, 2018, 34:35-45]
Artemisinin Suppressed Melanoma Recurrence and Metastasis after Radical Surgery through the KIT/PI3K/AKT Pathway [ Int J Biol Sci, 2025, 21(1):75-94] | PubMed: 39744440 |
Xiaoai Jiedu recipe reduces cell survival and induces apoptosis in hepatocellular carcinoma by stimulating autophagy via the AKT/mTOR pathway [ J Ethnopharmacol, 2025, 339:119135] | PubMed: 39586558 |
Vegfr3 activation of Pkd2l1+ CSF-cNs triggers the neural stem cell response in spinal cord injury [ Cell Signal, 2025, 130:111675] | PubMed: 39986360 |
A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109] | PubMed: 38714712 |
A pathological joint-liver axis mediated by matrikine-activated CD4+ T cells [ Signal Transduct Target Ther, 2024, 9(1):109] | PubMed: 38714712 |
Combined inhibition of HER2 and VEGFR synergistically improves therapeutic efficacy via PI3K-AKT pathway in advanced ovarian cancer [ J Exp Clin Cancer Res, 2024, 43(1):56] | PubMed: 38403634 |
Inhibition of Selenoprotein I promotes ferroptosis and reverses resistance to platinum chemotherapy by impairing Akt phosphorylation in ovarian cancer [ MedComm (2020), 2024, 5(12):e70033] | PubMed: 39669976 |
Jiawei Buzhong Yiqi Decoction attenuates polycystic ovary syndrome through regulating kisspeptin-GPR54-AKT-SHBG system [ Phytomedicine, 2024, 133:155931] | PubMed: 39116604 |
A novel DDIT3 activator dehydroevodiamine effectively inhibits tumor growth and tumor cell stemness in pancreatic cancer [ Phytomedicine, 2024, 155377] | PubMed: none |
Aberrant methylation and expression of TNXB promote chondrocyte apoptosis and extracullar matrix degradation in hemophilic arthropathy via AKT signaling [ Elife, 2024, 13RP93087] | PubMed: 38819423 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。