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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C12H15N3O4 |
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分子量 | 265.27 | CAS No. | 803647-40-7 | |
Solubility (25°C)* | 体外 | DMSO | 53 mg/mL (199.79 mM) | |
Ethanol | 53 mg/mL (199.79 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | NSC59984 is a p53 pathway activator via induction of mutant p53 protein degradation and p73 activation. |
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in vitro | NSC59984 specifically restores p53 pathway signaling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. NSC59984 also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells. [1] |
in vivo | In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1] |
細胞アッセイ | 細胞株 | SW480, DLD-1, HCT116, p53-null HCT116, MRC5, Hop92, RXF393 and Wi38 cells |
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濃度 | ~100 μM | |
反応時間 | 72 h | |
実験の流れ | Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager. | |
動物実験 | 動物モデル | Nude mice bearing DLD-1 xenograft tumors |
投薬量 | 45 mg/kg | |
投与方法 | i.p. |
DRP1 Inhibition Enhances Venetoclax-Induced Mitochondrial Apoptosis in TP53-Mutated Acute Myeloid Leukemia Cells through BAX/BAK Activation [ Cancers (Basel), 2023, 15(3)745] | PubMed: 36765703 |
Autophagy Inhibition in Trophoblasts Induces Aberrant Shift in CXCR4+ Decidual NK Cell Phenotype Leading to Pregnancy Loss [ J Clin Med, 2023, 12(23)7491] | PubMed: 38068544 |
Multi-Cell Type Glioblastoma Tumor Spheroids for Evaluating Sub-Population-Specific Drug Response [ Front Bioeng Biotechnol, 2020, 8:538663] | PubMed: 33042963 |
Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. [ Micromachines (Basel), 2020, 11(2)] | PubMed: 32085455 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。