受注:045-509-1970 |
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Synonyms | 1,1-Dimethylbiguanide | Storage (From the date of receipt) |
3 years -20°C powder | ||||
化学式 | C4H11N5 |
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分子量 | 129.16 | CAS No. | 657-24-9 | ||||
Solubility (25°C)* | 体外 | DMSO | 26 mg/mL (201.3 mM) | ||||
Water | 26 mg/mL (201.3 mM) | ||||||
Ethanol | 26 mg/mL (201.3 mM) | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Metformin (1,1-Dimethylbiguanide), a widely used drug for treatment of type 2 diabetes, activates AMP-activated protein kinase (AMPK) in hepatocytes. Metformin promotes mitophagy in mononuclear cells. Metformin induces apoptosis of lung cancer cells through activating JNK/p38 MAPK pathway and GADD153. |
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in vitro | Metformin upregulates BAX activation with facilitation of BIM, BAD and PUMA, downregulates Bcl-2 and Bcl-xl, also induces cytochrome c release from mitochondria into the cytoplasm, directly triggering caspase-9-mediated mitochondrial apoptosis.[4] |
in vivo | Metformin shows therapeutic effect of metformin in mouse model of liver fibrosis associated with nonalcoholic steatohepatitis (NASH) in which hepatic function, NASH lesions and fibrosis are improved.[4] |
細胞アッセイ | 細胞株 | human hepatic stellate cells (HSCs) LX-2, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A |
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濃度 | 0-80 mM | |
反応時間 | 24 h | |
実験の流れ | The human hepatic stellate cells (HSCs) LX-2 is maintained in RPMI 1640 medium, rat HSCs T6, human hepatocyte L02 and rat hepatocyte BRL-3A were maintained in DMEM, which are both supplemented with 10% (v/v) FBS and 1% (v/v) penicillin/streptomycin sulfate at 37 ℃ in a humidified 5% CO2 incubator. Metformin and AICAR solutions are filtered through 0.22 μm millipore membrane filter units. CCK8 assay is used to detect the effects of different concentration of metformin and AICAR on the proliferation of hepatic stellate cells. T6 cells and LX-2 cells are treated with different concentration of metformin and AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. Cells are treated with 10 mM metformin and 0.5 mM AICAR for 24 h, and the expression of collagen and α - SMA protein are detected by Western blot. The mRNA levels of collagen I and α - SMA are detected by RT-qPCR. |
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動物実験 | 動物モデル | Male C57BL/6N mice |
投薬量 | 10-200 mg/kg; 65 mg/kg | |
投与方法 | i.p. |
Pump-Less, Recirculating Organ-On-Chip (rOoC) Platform To Model The Metabolic Crosstalk Between Islets and Liver [ Adv Healthc Mater, 2024, e2303785.] | PubMed: 38221504 |
Activation of nemo-like kinase in diamond blackfan anemia suppresses early erythropoiesis by preventing mitochondrial biogenesis [ J Biol Chem, 2024, 300(8):107542] | PubMed: 38992436 |
A novel direct adenosine monophosphate kinase activator ameliorates disease progression in preclinical models of Autosomal Dominant Polycystic Kidney Disease [ Kidney Int, 2023, 103(5):917-929] | PubMed: 36804411 |
Metformin suppresses cardiac fibroblast proliferation under high-glucose conditions via regulating the mitochondrial complex I protein Grim-19 involved in the Sirt1/Stat3 signaling pathway [ Free Radic Biol Med, 2023, 206:1-12] | PubMed: 37353174 |
AMPK activator decelerates osteoarthritis development by inhibition of β-catenin signaling in chondrocytes [ J Orthop Translat, 2023, 38:158-166] | PubMed: 36439631 |
Resistin secreted by porcine alveolar macrophages leads to endothelial cell dysfunction during Haemophilus parasuis infection [ Virulence, 2023, 14(1):2171636] | PubMed: 36694280 |
Resveratrol Upregulates Senescence Marker Protein 30 by Activating AMPK/Sirt1-Foxo1 Signals and Attenuating H2O2-Induced Damage in FAO Rat Liver Cells [ J Nutr Sci Vitaminol (Tokyo), 2023, 10.3177/jnsv.69.388] | PubMed: 37940580 |
The NCF1 variant p.R90H aggravates autoimmunity by facilitating the activation of plasmacytoid dendritic cells [ The Journal of Clinical Investigation, 2022-, 132(16)] | PubMed: None |
Integrative analysis of drug response and clinical outcome in acute myeloid leukemia [ Cancer Cell, 2022, S1535-6108(22)00312-9] | PubMed: 35868306 |
Disrupting metformin adaptation of liver cancer cells by targeting the TOMM34/ATP5B axis [ EMBO Mol Med, 2022, 14(12):e16082] | PubMed: 36321555 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。