Homoharringtonine (HHT)

製品コードS9015 バッチS901507

印刷

化学情報

Chemical Structure Synonyms CGX-635, Myelostat, NSC 141633 Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C29H39NO9
分子量 545.62 CAS No. 26833-87-4
Solubility (25°C)* 体外 DMSO 100 mg/mL (183.27 mM)
Ethanol 25 mg/mL (45.81 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Homoharringtonine (HHT), a plant alkaloid with antitumor properties, inhibits protein translation by preventing the initial elongation step of protein synthesis via an interaction with the ribosomal A-site. Homoharringtonine reversiblely inhibits IL-6-induced STAT3 Tyrosine 705 phosphorylation and reduced anti-apoptotic proteins expression.
in vitro

Homoharringtonine (HHT), a plant alkaloid with antitumor properties, induces p62-mediated autophagy in resistant CML K562G cells, thus promoting autophagic degradation of the BCR-ABL protein.[1]
in vivo

Homoharringtonine (HHT) in combination with ABT-199, significantly prolongs overall survival of primary diffuse large B-cell lymphoma (DLBCL) xenograft-bearing mice compared with single-agent approaches.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 K562 cells, K562G cells
濃度 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml
反応時間 48 h
実験の流れ

K562, K562G, and siRNA-K562G cells are seeded in 384-well plates at a density of 8,000 cells/well, and then treated with 0.1% DMSO (vehicle control) or HHT at concentrations of 100, 50, 25, 12.5, 6.2, 3.1, 1.56, 0.78 and 0.39 ng/ml for 48 h. According to the manufacturer's instructions, cell proliferation is assayed using an MTS assay, and the absorbance is measured.
動物実験 動物モデル Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice of xenograft models of DLBCL
投薬量 50 µg/dose
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Comprehensive Analysis of the Tumour Immune Microenvironment in Canine Urothelial Carcinoma Reveals Immunosuppressive Mechanisms Induced by the COX-Prostanoid Cascade [ Vet Comp Oncol, 2024, 10.1111/vco.12999] PubMed: 39179510
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment [ iScience, 2023, 26(4):106543] PubMed: 37123244
Follicular lymphoma-associated mutations in the V-ATPase chaperone VMA21 activate autophagy creating a targetable dependency [ Autophagy, 2022, 1-19] PubMed: 35287545
The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma [ Cell Death Dis, 2022, 13-9:799] PubMed: 36123339
A survey of optimal strategy for signature-based drug repositioning and an application to liver cancer [ Elife, 2022, 11e71880] PubMed: 35191375
SiCoDEA: A Simple, Fast and Complete App for Analyzing the Effect of Individual Drugs and Their Combinations [ Biomolecules, 2022, 12(7)904] PubMed: 35883460
Establishment and Characterization of NCC-PMP1-C1: A Novel Patient-Derived Cell Line of Metastatic Pseudomyxoma Peritonei [ J Pers Med, 2022, 12(2)258] PubMed: 35207746
Homoharringtonine Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Signaling [ Mediators Inflamm, 2022, 2022:3441357] PubMed: 36211988

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。