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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder (seal) | |
化学式 | C80H126O44 |
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分子量 | 1791.83 | CAS No. | 15588-68-8 | |
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (55.8 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Gipsoside is a triterpene saponin from gypsophila paniculata L. |
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in vitro | Gipsoside has an activity of anti-inflammatory, anti-thrombotic, antioxidative and anti-cancer actions. Gypenoside inhibited SW-480 cell proliferation in a dose- and time-dependent manner. Gyp is capable of exerting different alternative cytotoxicity in cancer cells and normal cells, which might be potentially useful as a cancer preventive or treatment agent. Gyp could cause cell membrane integrity damage, decrease the Δψm level, induce DNA fragmentation and initiate apoptotic response in SW-480 cells. ROS generated in SW-480 cells play an important role in Gyp induced cell death. Gyp induces microfilament network collapse and injures the cell shape and migration ability[1]. It is reported that Gypenoside can induce neuroprotection against Aβ in vitro. Gypenoside attenuates Aβ-induced microglial activation, decreases the levels of microglial M1 state (classic activated state) markers, including iNOS protein expression, TNF-α, IL-1β, and IL-6 releases, and increases the levels of M2 markers, such as Arg-1 protein expression, IL-10, BDNF, and GDNF secretions from the cells. Gypenoside reduces the Aβ-induced microglial activation by shifting microglial M1 to M2 (alternative activated state) state, and the SOCS1 protein may mediate the process[2]. |
in vivo | Gipsoside has been known for its wide beneficial effects for treating hepatitis, hyperlipoproteinemia and cardiovascular disease[1]. |
細胞アッセイ | 細胞株 | SW-480 cells |
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濃度 | 0, 70, 100 and 130 µg/ml | |
反応時間 | 24 and 48 h | |
実験の流れ | To investigate the effect of Gypenoside on SW-480 cell proliferation, cells are seeded in 96-well plates. Various concentrations (0, 70, 100 and 130 µg/ml; 80% ethanol is used as the solvent control) of Gypenoside are added and the cells are incubated for various periods of time, at a density of 1×105 cells/ml, respectively. The cell viability is determined by using MTT assay. The absorbance at 570 nm is recorded using a microplate reader. |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。