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Synonyms | NCGC00099374 | Storage (From the date of receipt) |
3 years -20°C powder | |||
化学式 | C19H11F4N3OS2 |
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分子量 | 437.43 | CAS No. | 313380-27-7 | ||||
Solubility (25°C)* | 体外 | DMSO | 87 mg/mL (198.88 mM) | ||||
Water | Insoluble | ||||||
Ethanol | Insoluble | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM. |
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in vitro | FDI-6 (NCGC00099374) is characterized in depth and is shown to bind directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induces concomitant transcriptional down-regulation. Global transcript profiling of MCF-7 cells by RNA-seq shows that FDI-6 specifically down-regulates FOXM1-activated genes with FOXM1 occupancy confirmed by ChIP-seq.[1] |
in vivo | Inhibiting FOXM1 with FDI-6 inhibits liver cancer cell growth in vivo.[2] |
細胞アッセイ | 細胞株 | MCF-7 cells |
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濃度 | 20 μM, 40 μM | |
反応時間 | 6 h | |
実験の流れ | MCF-7 cells are grown in 15 cm2 dishes to 70% confluence prior to FDI-6 or DMSO control treatment for 6h. Cells are washed with PBS, cross-linked with 1% formaldehyde at RT for 10 min and quenched with 1/20 volume glycine (2.5 M). Cells are harvested by scraping into cold PBS containing protease inhibitors and cell pellets stored at −80 ℃. |
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動物実験 | 動物モデル | 4-week old male nude mice |
投薬量 | 25 mg/kg | |
投与方法 | intratumoral injection |
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A drug repurposing study identifies novel FOXM1 inhibitors with in vitro activity against breast cancer cells [ Med Oncol, 2024, 41(8):188] | PubMed: 38918225 |
Methionine regulates self-renewal, pluripotency, and cell death of GIC through cholesterol-rRNA axis [ BMC Cancer, 2022, 22(1):1351] | PubMed: 36564758 |
The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer [ Cell Death Dis, 2021, 12(10):944] | PubMed: 34650035 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。