Etonogestrel

製品コードS4673 バッチS467301

化学情報

	Synonyms	Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₂H₂₈O₂
	分子量	324.46	CAS No.	54048-10-1
Solubility (25°C)*	体外	DMSO	64 mg/mL (197.25 mM)
		Ethanol	64 mg/mL (197.25 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Etonogestrel (Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel) is a synthetic form of the naturally occurring female sex hormone progesterone. It binds to the cytoplasmic progesterone receptors in the reproductive system and subsequently activates progesterone receptor mediated gene expression.
in vitro	Etonogestrel induces FKBP51 mRNA and protein expression in cultured HESCs^[1].
in vivo	Etonogestrel (Implanon, Nexplanon, 3-Oxodesogestrel, 3-keto-Desogestrel) is a synthetic form of the naturally occurring female sex hormone progesterone.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human endometrial stromal cells (HESCs)
	濃度	100 nM
	反応時間	7 days
	実験の流れ	Thawed HESCs are grown to confluence in basal medium (BM), a phenol red-free 1:1 v/v mix of Dulbecco's MEM/Ham's F-12, with 100U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml fungizone complex supplemented with 10% charcoal-stripped calf serum. Confluent HESCs are incubated in parallel in BM with 0.1% ethanol (vehicle control) or 10nM E2(estradiol) or E2+100 nM P4(progesterone), or 100 nM of ETO(etonogestrel) or 100 nM of MPA(medroxyprogesterone acetate). After 7 days, the cultures are washed twice with 1X phosphate-buffered saline (PBS) to remove residual serum, then placed in a serum-free defined media (DM) comprising BM plus ITS+ (insulin, transferrin, selenious and linoleic acid) premix, 5 μM of FeSO4, 50 μM of ZnSO4, 1 nM of CuSO4, 20 nM of Na2SeO3 trace elements, 50 μg/ml of ascorbic acid and 50 ng/ml of recombinant epidermal growth factor for 24h. The cultures are washed twice with 1X PBS to remove the residual steroids and DM containing corresponding vehicle control or steroids is added to the cultures for 6h or 24h. At the end of incubation periods, HESCs are washed with ice-cold 1X PBS and stored at −70°C until used for total RNA and protein extraction.
動物実験	動物モデル	Wistar-Albino rats
	投薬量
	投与方法

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Next-Generation Contraceptive Intravaginal Ring: Comparison of Etonogestrel and Ethinyl Estradiol In Vitro and In Vivo Release from 3D-Printed Intravaginal Ring and NuvaRing [ Pharmaceutics, 2024, 16(8)1030]	PubMed: 39204375
Next generation 3D-printed intravaginal ring for prevention of HIV and unintended pregnancy [ Biomaterials, 2023, 301:122260]	PubMed: 37549505
Transcriptionally regulated miR-26a-5p may act as BRCAness in Triple-Negative Breast Cancer [ Breast Cancer Res, 2023, 25(1):75]	PubMed: 37365643
Long-Acting Technologies for Prevention of HIV and Unplanned Pregnancy [ ProQuest, 2023, 30314559]	PubMed: none

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。