EC330

製品コードS0472 バッチS047202

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₃₀H₃₂F₂O₂

分子量

462.57

CAS No.

2016795-77-8

Solubility (25°C)*

体外

Ethanol

93 mg/mL (201.05 mM)

DMSO

23 mg/mL (49.72 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 23 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 23 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	EC330 is a steroidal inhibitor of leukemia inhibitory factor (LIF, an interleukin 6 class cytokine). EC330 inhibits the LIF/LIF-R signaling and blocks the promoting effects of LIF on growth and migration of cancer cells.
in vitro	EC330, a potential LIF inhibitor, inhibits the activation of STAT3, PI3K/AKT, and mTOR signaling pathways by LIF, preferentially inhibits the proliferation of cancer cells with LIF overexpression, also inhibits LIF function in promoting migration of cancer cells.^[1]
in vivo	EC330 inhibits multi-functional cytokine LIF function in promoting tumor growth in vivo.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	MCF7, MDA-MB231 cell lines
	濃度	--
	反応時間	24 h
	実験の流れ	The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was determined by counting cell numbers daily for four days.
動物実験	動物モデル	BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression
	投薬量	1 mg/kg
	投与方法	i.p.

参考

[1] Xuetian Yue, et al. J Mol Cell Biol. 2020 Jul 3;12(6):477-480.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer [ Br J Cancer, 2021, 10.1038/s41416-021-01631-3]	PubMed: 34815524

PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer [ Br J Cancer, 2021, 10.1038/s41416-021-01631-3]

PubMed: 34815524

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。