Doxycycline HCl

製品コードS4005 バッチS400502

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C22H24N2O8.HCl
分子量 480.9 CAS No. 10592-13-9
Solubility (25°C)* 体外 DMSO (warmed with 50ºC water bath) 96 mg/mL (199.62 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
4.8mg/ml Taking the 1 mL working solution as an example, add 50 μL of 96 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
0.6mg/ml Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

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生物活性

製品説明 Doxycycline HCl is a tetracycline antibiotic that commonly used to treat a variety of infections, and also a MMP inhibitor.
in vitro Doxycycline (50 μM) completely inhibits degradation of type II collagen, but not that of type I collagen in rheumatoid synovial fibroblasts and endothelial cells. Doxycycline (50 μM) down-regulated MMP-8 induction, at both the mRNA and protein levels in rheumatoid synovial fibroblasts and endothelial cells. [1] Doxycycline not only inhibits MMP-8 and MMP-9 (gelatinase B) activity, but also the synthesis of MMPs in human endothelial cells. Doxycycline (50 μM) completely inhibits the phorbol-12-myristate-13-acetate (PMA)-mediated induction of MMP-8 and MMP-9, as measured by Western blotting and gelatin zymography, respectively. [2] Doxycycline inhibits the LPS-induced IL-1beta increase in the mRNA and protein amounts in the corneal epithelium and upregulates the expression of the anti-inflammatory IL-1 RA protein in human cultured corneal epithelium. Doxycycline reduces the steady state level of the cellular ICE protein but does not affect the level of ICE transcripts. Doxycycline significantly decreases IL-1beta bioactivity in the supernatants from LPS-treated corneal epithelial cultures.[3]
in vivo Doxycycline (25 mg/day) prevents the structural deterioration of aortic elastin without decreasing the influx of inflammatory cells in Wistar rats. Doxycycline (25 mg/day) markedly suppresses increased aortic wall production of 92 kD gelatinase in Wistar rats. [4] Doxycycline reduces the activity of matrix metalloproteinase (MMP)-2 and MMP-9 in the arterial wall, and inhibits smooth muscle cell migration from media to intima by 77% at 4 days after balloon injury in rats. Doxycycline reduces the replication of smooth muscle cells in the intima in rats. [5]

プロトコル(参考用のみ)

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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