Cycloheximide

製品コードS7418 バッチS741802

印刷

化学情報

Chemical Structure Synonyms NSC-185, Actidione, Naramycin A, CHX, FT 3422-2, NM-MCD 80 Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent
化学式

C15H23NO4
分子量 281.35 CAS No. 66-81-9
Solubility (25°C)* 体外 DMSO 56 mg/mL (199.04 mM)
Ethanol 56 mg/mL (199.04 mM)
Water 15 mg/mL (53.31 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Cycloheximide (NSC-185) can be used to induce animal models of Dysmnesia.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro

Cyclohexamide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.[1]
in vivo

Cycloheximide, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 grouper fin cell line (GF-1)
濃度 0.33 µg/ml
反応時間 0-5 days
実験の流れ

Monolayers of GF-1 cells (4.0 mL, 105 cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
動物実験 動物モデル Male ICR mice
投薬量 30mg/kg, 60mg/kg,120 mg/kg
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Effective targeting of PDGFRA-altered high-grade glioma with avapritinib [ Cancer Cell, 2025, S1535-6108(25)00070-4] PubMed: 40086436
VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26] PubMed: 39848960
The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis [ Nat Commun, 2025, 16(1):2039] PubMed: 40016204
ACSS2 drives senescence-associated secretory phenotype by limiting purine biosynthesis through PAICS acetylation [ Nat Commun, 2025, 16(1):2071] PubMed: 40021646
The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism [ Mol Cell, 2025, 85(3):638-651.e9] PubMed: 39889707
OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model [ Adv Sci (Weinh), 2025, 12(7):e2406700] PubMed: 39721018
Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1 [ Theranostics, 2025, 15(7):3207-3222] PubMed: 40083918
Glutathione reductase underlies the stability of mutant p53 by antagonizing protein glutathionylation [ Redox Biol, 2025, 81:103522] PubMed: 39983342
Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein [ Research (Wash D C), 2025, 8:0626] PubMed: 40007621
Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway [ J Adv Res, 2025, S2090-1232(25)00038-4] PubMed: 39805423

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。