Cycloheximide

製品コードS7418 バッチS741802

印刷

化学情報

Chemical Structure Synonyms NSC-185, Actidione, Naramycin A, CHX, FT 3422-2, NM-MCD 80 Storage
(From the date of receipt)		 3 years -20°C(in the dark) powder
1 year -80°C(in the dark) in solvent
化学式

C15H23NO4
分子量 281.35 CAS No. 66-81-9
Solubility (25°C)* 体外 DMSO 56 mg/mL (199.04 mM)
Ethanol 56 mg/mL (199.04 mM)
Water 15 mg/mL (53.31 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
in vitro

Cyclohexamide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.[1]
in vivo

Cycloheximide, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 grouper fin cell line (GF-1)
濃度 0.33 µg/ml
反応時間 0-5 days
実験の流れ

Monolayers of GF-1 cells (4.0 mL, 105 cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
動物実験 動物モデル Male ICR mice
投薬量 30mg/kg, 60mg/kg,120 mg/kg
投与方法 i.p.

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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