受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C17H27ClN8O5 |
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分子量 | 458.9 | CAS No. | 3513-03-9 | |
Solubility (25°C)* | 体外 | Water | 91 mg/mL (198.3 mM) | |
DMSO | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used to select transfected cells carrying bsr or BSD resistance genes. |
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in vitro | Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. [1] Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. [2] Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. [3] Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. [4] |
in vivo | Blasticidin S HCl is an antibiotic isolated from Stretomyces girseochromogenes that acts as a protein synthesis inhibitor. It is used to select transfected cells carrying bsr or BSD resistance genes. |
細胞アッセイ | 細胞株 | HEK293T cells |
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濃度 | 5 µg/ml | |
反応時間 | 7 days | |
実験の流れ | Lentivirus infected cells were maintained in 5 μg/ml blasticidin containing culture medium for 7d, with fresh medium exchange every day. |
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動物実験 | 動物モデル | Mice |
投薬量 | 2.5 mg/kg | |
投与方法 | Oral |
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YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the mediator complex in colorectal cancer [ Pharmacol Res, 2024, 206:107283] | PubMed: 38964523 |
Chicoric Acid Ameliorated Beta-Amyloid Pathology and Enhanced Expression of Synaptic-Function-Related Markers via L1CAM in Alzheimer's Disease Models [ Int J Mol Sci, 2024, 25(6)3408] | PubMed: 38542381 |
Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51 [ Nat Commun, 2023, 10.1038/s41467-023-42850-x] | PubMed: 37973845 |
Genetically encoded photocatalytic protein labeling enables spatially-resolved profiling of intracellular proteome [ Nat Commun, 2023, 14(1):2978] | PubMed: 37221179 |
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] | PubMed: 37833415 |
Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin [ Mol Cell, 2023, 83(13):2206-2221.e11] | PubMed: 37311463 |
Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin [ Mol Cell, 2023, 83(13):2206-2221.e11] | PubMed: 37311463 |
Targeting VCP potentiates immune checkpoint therapy for colorectal cancer [ Cell Rep, 2023, 42(11):113318] | PubMed: 37865914 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。