受注:045-509-1970 |
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Synonyms | ALS-357, Lupatic acid, Betulic acid | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C30H48O3 |
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分子量 | 456.7 | CAS No. | 472-15-1 | |
Solubility (25°C)* | 体外 | DMSO | 47 mg/mL (102.91 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Betulinic acid (ALS-357, Lupatic acid, Betulic acid), a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M. It's reported that Betulinic acid acts as a new activator of NF-κB.Phase 1/2. |
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in vitro | Betulinic acid shows anti-HIV activity by blocking HIV replication in H9 lymphocytes with an EC50 value of 1.4 μM and inhibiting uninfected H9 cell growth with an IC50 value of 13 μM. [1] Betulinic acid also acts as anti-melanoma agent through inhibiting aminopeptidase N activity with IC50 of 7.3 μM. [2] In addition, Betulinic acid inhibits eukaryotic topoisomerase I activity with IC50 of 5 μM and can be exploited as a strong candidate for anti-tumor drug. [3] |
in vivo | Betulinic acid inhibits prostate cancer cell and tumor (xenograft) growth in athymic nude mice bearing LNCaP cells and this is due, in part, to proteasome-dependent downregulation of Sp1, Sp3, Sp4 and several Sp-regulated genes. [4] |
キナーゼアッセイ | Topoisomerase I-DNA interaction | |
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Annealed 25-mer duplex of oligonucleotide-1 and oligonicleotide-2 is incubated with 25 or 50 units of rat liver topoisomerase I at 8 °C for 15 min in the presence or absence of betulinic acid in binding buffer. After incubation the reaction mixtures are electrophoresed in 7% non-denaturing polyacrylamide gel at 4 °C in 0.167 × TBE buffer and DNA bands are stained with ethidium bromid | ||
細胞アッセイ | 細胞株 | H9 lymphocytes |
濃度 | ||
反応時間 | 3 days | |
実験の流れ | H9 lymphocytes ( 1 × 107 cells/ml) are incubated in the presence or absence of HIV-1 (IIIB isolate) for 1 h at 37 °C. Cells are washed thoroughly to remove unadsorbed virions and resuspended in culture medium. Betulinic acid (diluted in test medium) is added to the culture medium. After incubation for 3 days at 37 °C, the cell densities of uninfected cultures are determined to assess toxicity of betulinic acid. A p24 antigen capture assay is used to determine the level of HIV infection in HIV-treated culture | |
動物実験 | 動物モデル | LNCaP tumor xenograft model |
投薬量 | 10 and 20 mg/kg/d | |
投与方法 | Orally |
Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice [ World J Stem Cells, 2024, 16(5):575-590] | PubMed: 38817328 |
Glaucocalyxin A delays the progression of OA by inhibiting NF-κB and MAPK signaling pathways [ J Orthop Surg Res, 2024, 19(1):188] | PubMed: 38500177 |
Graphene oxide/gallium nanoderivative as a multifunctional modulator of osteoblastogenesis and osteoclastogenesis for the synergistic therapy of implant-related bone infection [ Bioact Mater, 2023, 25:594-614] | PubMed: 37056253 |
N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1 [ J Neuroinflammation, 2023, 20(1):91] | PubMed: 37029422 |
Embelin attenuates lipopolysaccharide-induced acute kidney injury through the inhibition of M1 macrophage activation and NF-κB signaling in mice [ Heliyon, 2023, 9(3):e14006] | PubMed: 36938407 |
Citrulline inhibits LPS-induced pyroptosis of RAW264.7 macrophages through NF-κB signaling pathway [ Immun Inflamm Dis, 2023, 11(4):e832] | PubMed: 37102651 |
TAZ ameliorates the microglia-mediated inflammatory response via the Nrf2-ROS-NF-κB pathway [ Mol Ther Nucleic Acids, 2022, 28:435-449] | PubMed: 35505966 |
Spironolactone Ameliorates Senescence and Calcification by Modulating Autophagy in Rat Tendon-Derived Stem Cells via the NF-κB/MAPK Pathway [ Oxid Med Cell Longev, 2021, 2021:5519587] | PubMed: 34306308 |
Betulinic acid promotes the osteogenic differentiation of human periodontal ligament stem cells by upregulating EGR1 [ Acta Biochim Biophys Sin (Shanghai), 2021, gmab111] | PubMed: 34519779 |
Long Noncoding RNA MALAT1 Regulates the Progression of Atherosclerosis by miR-330-5p/NF-κB Signal Pathway [ J Cardiovasc Pharmacol, 2021, 78(2):235-246] | PubMed: 34554676 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。