Acetylcholine iodide

製品コードS4718

印刷

化学情報

Chemical Structure Synonyms Acetylcolina Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C7H16NO2.I
分子量 273.11 CAS No. 2260-50-6
Solubility (25°C)* 体外 DMSO 54 mg/mL (197.72 mM)
Water 54 mg/mL (197.72 mM)
Ethanol '54 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Acetylcholine iodide (Acetylcolina) is a neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
in vitro Acetylcholine iodide (Ach) stimulated SBC3 cell proliferation, adhesion and migration toward fibronectin (Fn) in a dose-dependent manner[1]. ACh ameliorates TNF-α-induced calpain activation by decreasing p38-MAPK phosphorylation and enhancing calpastatin expression. It elicits an anti-apoptotic effect through the activation of the muscarinic ACh receptor (MAChR) and the activation of anti-oxidant systems. ACh increases cell viability and decreases TNF-α-induced apoptosis in H9c2 cells[2].
in vivo Acetylcholine can inhibit pro-inflammatory cytokine release and protect against cardiomyocyte injury[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 The SBC3, human SCLC cell line
濃度 1, 10, 100 μM
反応時間 24, 48 or 72 h
実験の流れ The SBC3 cells are seeded at 2000 cells/well in treated 96-well plates maintained with RPMI1640 medium containing 10% (v/v) FCS for 24 h. The medium is removed and replaced with 100 μl RPMI1640 containing 1% (v/v) ITS. The cells are incubated in serum free medium for 24 h to synchronize the cell cycle. Then another 100 μl serum-free medium containing the agonist/antagonist under investigation is added. The antagonist is added 30 min before Ach(Acetylcholine iodide). At this time, the cells in a duplicate plate are given 100 μl serum-free medium and a MTT assay is used to determine live cell numbers before treatment with the agonist/antagonist as measured by the absorbance (OD) at 550 nm/650 nm. After 24, 48 or 72 h culture, the remaining cells are assayed to yield post-agonist/antagonist cell numbers using the same method. All the experiments are performed in triplicate.
動物実験 動物モデル Female albino rats
投薬量 25 or 50 mg/kg
投与方法 s.c

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Vitamin D ameliorates asthma-induced lung injury by regulating HIF-1α/Notch1 signaling during autophagy [ Food Sci Nutr, 2022, 10(8):2773-2785] PubMed: 35959262
Crosstalk between PKC and MAPK pathway activation in cardiac fibroblasts in a rat model of atrial fibrillation [ Biotechnol Lett, 2020, 42(7):1219-1227] PubMed: 32095918

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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