Thiazovivin

製品コードS1459 バッチS145902

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₅H₁₃N₅OS
	分子量	311.36	CAS No.	1226056-71-8
Solubility (25°C)*	体外	DMSO	15 mg/mL (48.17 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.
in vitro	Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. ^[1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. ^[2]

製品説明

Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.

in vitro

Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. ^[1]

Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. ^[2]

プロトコル(参考用のみ)

キナーゼアッセイ	In vitro ROCK assay
キナーゼアッセイ	Thiazovivin is dissolved in DMSO. CycLex Rho-kinase assay kit is used to detect ROCK activity using recombinant ROCK in the presence of increasing concentrations of Thiazovivin (~10 μM).
細胞アッセイ	細胞株	ASZ001 cells
	濃度	0.5 μM
	反応時間	72 h
	実験の流れ	Cells were treated with various concnetrations of thiazovivin.

参考

[4] Whitson RJ, et al. Nat Med. 2018 Mar;24(3):271-281.

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カスタマーフィードバック

: Data from [Arch Toxicol, 2013, 87(12), 2215-31]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells [ Nat Biotechnol, 2024, 10.1038/s41587-024-02360-7]	PubMed: 39223325
METTL3/METTL14 maintain human nucleoli integrity by mediating SUV39H1/H2 degradation [ Nat Commun, 2024, 15(1):7186]	PubMed: 39169036
HBO1 determines SMAD action in pluripotency and mesendoderm specification [ Nucleic Acids Res, 2024, gkae158]	PubMed: 38421638
Generation of human vascularized and chambered cardiac organoids for cardiac disease modelling and drug evaluation [ Cell Prolif, 2024, e13631.]	PubMed: 38453465
RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo [ Cell Mol Life Sci, 2024, 81(1):92]	PubMed: 38363375
Telomerase is essential for cardiac differentiation and sustained metabolism of human cardiomyocytes [ Cell Mol Life Sci, 2024, 81(1):196]	PubMed: 38658440
Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells [ iScience, 2024, 27(1):108599]	PubMed: 38170020
Large-scale generation of IL-12 secreting macrophages from human pluripotent stem cells for cancer therapy [ Mol Ther Methods Clin Dev, 2024, 32(1):101204]	PubMed: 38390556
Establishment of a human induced pluripotent stem cell line from a patient with dilated cardiomyopathy [ Stem Cell Res, 2024, 78:103467]	PubMed: 38861774
Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation [ Stem Cell Res, 2024, 77:103404]	PubMed: 38552356

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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