Daidzin

製品コードS2289 バッチS228903

印刷

化学情報

Chemical Structure Synonyms Daidzoside, Daidzein 7-O-glucoside, Daidzein 7-glucoside Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C21H20O9

分子量 416.38 CAS No. 552-66-9
Solubility (25°C)* 体外 DMSO 83 mg/mL (199.33 mM)
Ethanol 4 mg/mL (9.6 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Daidzin (Daidzoside, Daidzein 7-O-glucoside, Daidzein 7-glucoside), a natural organic compound in the class of phytochemicals known as isoflavones, is a potent and selective inhibitor of human mitochondrial aldehyde dehydrogenase and inhibits ALDH-I selectively (Ki=20 nM); at least 500 times less effective against ALDH-Ⅱ, the cytosolic isozyme (Ki=10 μM).
in vitro Daidzin, a glycoside of daidzein, increases the transcriptional activity of RARα and RARγ but does not bind to the RARs[1]. Daidzin does not inhibit human class Ⅰ, Ⅱ, or Ⅲ alcohol dehydrogenases, nor does it have any significant effect on biological systems that are known to be affected by other isoflavones. Daidzin inhibits human ALDH-I and ALDH-II in a concentration-dependent manner. Daidzin inhibits both ALDH-I and ALDH-II in an apparently competitive manner with Ki values of 40 nM and 20 μM, respectively, and it inhibits ALDH-I uncompetitively with respect to NAD+. The inhibition of ALDH-I by daidzin is reversible[3].
in vivo Daidzin has no effect on alcohol-metabolizing enzymes(i.e., ADH and ALDH) when given to rats intragastrically. Chronic daidzin administration exerts an effect on alcohol pharmacokinetics, although the effect is less pronounced than when the compound is administered concurrently with ethanol.The compound is shown to shorten sleep time if ethanol is given intragastrically, but not when given intraperitoneally, indicating absence of effect on ethanol elimination rate. Daidzin delays ethanol absorption and lessens alcohol intoxication. The compound is shown to suppress the levels of BAC(blood alcohol concentration) for the first 3 hr after alcohol ingestion in both fasted and fed rats. These effects of daidzin may in part be due to its antioxidant activity[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 CV-1 cells
濃度 0.5, 1, 10, 50, and 100 μM
反応時間 24 h
実験の流れ

CV-1 cells are transfected with 100 ng ERE-Luc and 50 ng ER-RARα or ER-RARγ. Transfected cells are treated with the indicated isoflavone at 0.5, 1, 10, 50, and 100 μM for 24 h. The β-Gal activity is used to normalize luciferase activity. 
動物実験 動物モデル Male Wistar rats
投薬量 30 mg/kg
投与方法 intragastrically

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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