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Synonyms | RO215535, Topitriol, 1,25-Dihydroxyvitamin D3 | Storage (From the date of receipt) |
1 year -80°C powder(in dark and seal) | |
化学式 | C27H44O3 |
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分子量 | 416.64 | CAS No. | 32222-06-3 | |
Solubility (25°C)* | 体外 | DMSO | 83 mg/mL (199.21 mM) | |
Ethanol | 83 mg/mL (199.21 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Calcitriol is a nonselective vitamin D receptor activator/agonist(VDRA), exhibiting a 10-fold higher vitamin D receptor (VDR) binding affinity(IC50=0.4 nM) than the selective VDRA paricalcitol.Solutions are unstable and should be fresh-prepared. |
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in vitro | Calcitriol is a potent inhibitor of PHA-induced lymphocyte proliferation, achieving 70% inhibition of tritiated thymidine incorporation after 72 h in culture. Calcitriol suppresses interleukin-2 (IL-2) production by PHA-stimulated peripheral blood mononuclear cells in a concentration-dependent fashion. [1] Calcitriol increases the concentration of intracellular calcium ([Ca2+]i) within 5 s by mobilizing calcium from the endoplasmic reticulum and the formation of inositol 1,4, 5-trisphosphate and diacylglycerol. [2] Calcitriol can inhibit the proliferation and promote the differentiation of human prostate adenocarcinoma cells. Calcitriol causes a selective decrease in the secreted levels of type IV collagenases (MMP-2 and MMP-9). [3] Calcitriol has antiproliferative activity in squamous cell carcinoma and prostatic adenocarcinoma and enhances the antitumor activity of platinum-based agents. Calcitriol prior to paclitaxel significantly reduces clonogenic survival compared with either agent alone in the murine squamous cell carcinoma and PC-3 cells. [4] Calcitriol is a potent anti-proliferative agent in a wide variety of malignant cell types. Calcitriol effects are associated with an increase in G0/G1 arrest, induction of apoptosis and differentiation, modulation of expression of growth factor receptors. Calcitriol potentiates the antitumor effects of many cytotoxic agents and inhibits motility and invasiveness of tumor cells and formation of new blood vessels. [5] |
細胞アッセイ | 細胞株 | CLL cells |
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濃度 | 100 nM | |
反応時間 | 24 h | |
実験の流れ | CLL cells were treated with DMSO (vehicle control) or calcitriol (100 nM). Cells were harvested at the indicated time points with mild trypsinization |
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動物実験 | 動物モデル | Male Sprague–Dawley rats |
投薬量 | 200 ng/kg | |
投与方法 | p.o. |
Data independently produced by , , Mireia Vila Gasull University of Porto. 2011;Mireia Vila Gasull
Data from [Data independently produced by , , Mol Cell Endocrinol, 2018, 470:1-13]
Data from [Data independently produced by , , Inflammation, 2017, 40(6):1884-1893]
VDR regulates mitochondrial function as a protective mechanism against renal tubular cell injury in diabetic rats [ Redox Biol, 2024, 70:103062] | PubMed: 38320454 |
Calcitriol impairs the secretion of IL-4 and IL-13 in Th2 cells via modulating VDR-Gata3-Gfi1 axis [ bioRxiv, 2024, ] | PubMed: none |
In Vivo and In Vitro Evidence for an Interplay between the Glucocorticoid Receptor and the Vitamin D Receptor Signaling [ Cells, 2023, 12(18)2291] | PubMed: 37759513 |
In Vivo and In Vitro Evidence for an Interplay between the Glucocorticoid Receptor and the Vitamin D Receptor Signaling [ Cells, 2023, 12(18)2291] | PubMed: 37759513 |
Vitamin A- and D-Deficient Diets Disrupt Intestinal Antimicrobial Peptide Defense Involving Wnt and STAT5 Signaling Pathways in Mice [ Nutrients, 2023, 15(2)376] | PubMed: 36678247 |
Brk/PTK6 and Involucrin Expression May Predict Breast Cancer Cell Responses to Vitamin D3 [ Int J Mol Sci, 2023, 24(13)10757] | PubMed: 37445934 |
Brk/PTK6 and Involucrin Expression May Predict Breast Cancer Cell Responses to Vitamin D3 [ Int J Mol Sci, 2023, 24(13)10757] | PubMed: 37445934 |
Piscine Vitamin D Receptors Vdra/Vdrb in the Absence of Vitamin D Are Utilized by Grass Carp Reovirus for Promoting Viral Replication [ Microbiol Spectr, 2023, 11(4):e0128723] | PubMed: 37466438 |
Piscine Vitamin D Receptors Vdra/Vdrb in the Absence of Vitamin D Are Utilized by Grass Carp Reovirus for Promoting Viral Replication [ Microbiol Spectr, 2023, 11(4):e0128723] | PubMed: 37466438 |
Vitamin D3 suppresses the cholesterol homeostasis pathway in patient-derived glioma cell lines [ FEBS Open Bio, 2023, 13(9):1789-1806] | PubMed: 37489660 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。