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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
化学式 | C18H12Cl2N4O4S |
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分子量 | 451.28 | CAS No. | 315706-13-9 | |
Solubility (25°C)* | 体外 | DMSO | 90 mg/mL (199.43 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | 4EGI-1 is a competitive eIF4E/eIF4G interaction inhibitor by binding to eIF4E with KD of 25 μM. 4EGI-1 specifically inhibits the function of mTOR by blocking the activation of 4E-BP1. 4EGI-1 induces apoptosis. |
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in vitro | 4EGI-1 disrupts the eIF4F complex and inhibits Cap-dependent translation in vitro. 4EGI-1 has proapoptotic activity in Jurkat cells, and potently inhibits cell growth with IC50 of approximately 6 μM in A549 lung cancer cells. [1] 4EGI-1 augments TRAIL-induced apoptosis through induction of DR5 and down-regulation of c-FLIP, independent of inhibition of cap-dependent protein translation in human lung cancer cells. [2] In addition, 4EGI-1, restores sensitivity to ABT-737 apoptosis through cap-dependent and -independent mechanisms in chronic lymphocytic leukemia. [3] |
細胞アッセイ | 細胞株 | Jurkat cells and A549 lung cancer cells. |
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濃度 | ~60 μM | |
反応時間 | 24 hours | |
実験の流れ | Cell viability is measured by treatment of Jurkat cells with compound for 24 h and by determination of intracellular ATP using the CellTiterGlo assay. For measurement of apoptotic DNA fragmentation, cells are treated for 24 h with 60 μM EGI-1 or 6.65 μM camptothecin in the presence or absence of 100 mM zVAD-FMK, a broad-spectrum caspase inhibitor. After fixation and staining with PI, cellular DNA content is determined by FACS analysis in a FACS Calibur machine. Nuclear morphology after 24 h EG1-1 treatment is visualized by staining of cells with Hoechst dye and fluorescence microscopy. For the A549 lung cancer cells, cell growth in the presence of 4EGI-1 is determined using the SRB staining method. |
Data from [Data independently produced by , , Mol Cancer Ther, 2017, 16(9):1806-1818]
Data from [Data independently produced by , , Int J Oncol, 2015, 47(6):2217-25.]
mTORC1 regulates cell survival under glucose starvation through 4EBP1/2-mediated translational reprogramming of fatty acid metabolism [ Nat Commun, 2024, 15(1):4083] | PubMed: 38744825 |
mTORC1 regulates cell survival under glucose starvation through 4EBP1/2-mediated translational reprogramming of fatty acid metabolism [ Nat Commun, 2024, 15(1):4083] | PubMed: 38744825 |
DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation [ Brain Sci, 2023, 13(5)745] | PubMed: 37239217 |
DEAD-Box Helicase 17 Promotes Amyloidogenesis by Regulating BACE1 Translation [ Brain Sci, 2023, 13(5)745] | PubMed: 37239217 |
Surgical Reconstruction of Stage 3 and 4 Pressure Injuries: A Literature Review and Proposed Algorithm from an Interprofessional Working Group [ Adv Skin Wound Care, 2023, 36(5):249-258] | PubMed: 37079788 |
LRG1 is a novel HER3 ligand to promote growth in colorectal cancer [ bioRxiv, 2023, PPR618443 ] | PubMed: none |
Ribosome Quality Control Mechanism Mitigates the Cytotoxic Impacts of Ribosome Collisions Induced by 5-Fluorouracil [ bioRxiv, 2023, 10.1101/2023.12.26.573247] | PubMed: none |
Dephosphorylation of 4EBP1/2 Induces Prenatal Neural Stem Cell Quiescence [ bioRxiv, 2023, 2023.02.14.528513] | PubMed: 36824760 |
Retinoic acid and RARγ maintain satellite cell quiescence through regulation of translation initiation [ Cell Death Dis, 2022, 13-9:838] | PubMed: 36175396 |
Role of EIF4G1 network in non-small cell lung cancers (NSCLC) cell survival and disease progression [ J Cell Mol Med, 2021, 10.1111/jcmm.16307] | PubMed: 33539648 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。