2-DG (2-Deoxy-D-glucose)

製品コードS4701 バッチS470113

化学情報

	Synonyms	2-deoxyglucose, NSC 15193, 2-Deoxy-D-arabino-hexose, D-Arabino-2-deoxyhexose	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₆H₁₂O₅
	分子量	164.16	CAS No.	154-17-6
Solubility (25°C)*	体外	DMSO	32 mg/mL (194.93 mM)
		Water	32 mg/mL (194.93 mM)
		Ethanol	8 mg/mL (48.73 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	2-DG (2-Deoxy-D-glucose), an analog of glucose, is a glycolytic inhibitor with antiviral activity. 2-Deoxy-D-glucose induces apoptosis and inhibits Herpes Simplex Virus type-1 (HSV-1) receptor expression.
in vitro	2-Deoxy-D-glucose(2-DG) activates AKT function through phosphatidylinositol 3-kinase (PI3K) and is independent of glycolysis or mTOR inhibition. 2-DG treatments disrupts the binding between insulin-like growth factor 1 (IGF-1) and IGF-binding protein 3 (IGFBP3) so that the free form of IGF-1 could be released from the IGF-1·IGFBP3 complex to activate IGF-1 receptor (IGF1R) signaling. 2-DG-induced activation of many survival pathways can be jointly attenuated through IGF1R inhibition. 2-DG also induces time- and dose-dependent ERK phosphorylation^[1]. 2-DG is readily transported into cells and is phosphorylated by hexokinase, but cannot be metabolized further and accumulates in the cell. This leads to ATP depletion and the induction of cell-death^[2]. 2DG significantly suppresses proliferation, causes apoptosis and reduces migration of murine endothelial cells, inhibiting formation of lamellipodia and filopodia and causing disorganization of F-actin filaments in murine endothelial cell^[5].
in vivo	Treatment of cancer patients with relatively high doses of 2-DG (greater than 200 mg/kg) was largely ineffective in managing tumor growth. Side effects of 2-DG included elevated blood glucose levels, progressive weight loss with lethargy, and behavioral symptoms of hypoglycemia^[2]. 2-DG enhances isoflurane-induced loss of righting reflex in mice. By reducing metabolism, 2-DG treatment can decrease body temperature in rodent, enhancing sensitivity to anesthetics^[3]. 2-DG diet significantly increased serum ketone body level and brain expression of enzymes required for ketone body metabolism. The 2-DG-induced maintenance of mitochondrial bioenergetics was paralleled by simultaneous reduction in oxidative stress. Further, 2-DG treated mice exhibited a significant reduction of both amyloid precursor protein (APP) and amyloid beta (Aβ) oligomers, which was paralleled by significantly increased α-secretase and decreased γ-secretase expression, indicating that 2-DG induced a shift towards a non-amyloidogenic pathway. 2-DG increased expression of genes involved in Aβ clearance pathways, degradation, sequestering, and transport. Concomitant with increased bioenergetic capacity and reduced β-amyloid burden, 2-DG significantly increased expression of neurotrophic growth factors, BDNF and NGF, thus reduces pathology in female mouse model of Alzheimer's disease^[4].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	H460 or H157 cells
	濃度	5 mM
	反応時間	48 h
	実験の流れ	2×10³ H460 or H157 cells are seeded in 96-well cell culture plates. Cells are treated with 5 mM 2-DG only, 5 or 10 μM IGF1R inhibitor II only, or a combination of 2-DG and IGF1R inhibitor II. Cell growth inhibition is determined after 48 h by the CellTiter 96® AQueous nonradioactive cell proliferation assay.
動物実験	動物モデル	Adult C57BL/6J mice
	投薬量	1000 mg/kg
	投与方法	i.p.

参考

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カスタマーフィードバック

: Data from [Data independently produced by , , Int J Oncol, 2018, 52(6):1899-1911]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

RIG-I is an intracellular checkpoint that limits CD8+ T-cell antitumour immunity [ EMBO Mol Med, 2024, 10.1038/s44321-024-00136-9]	PubMed: 39322862
Tumor-derived Exosomal ENO2 Modulates Polarization of Tumor-associated Macrophages through Reprogramming Glycolysis to Promote Progression of Diffuse Large B-cell Lymphoma [ Int J Biol Sci, 2024, 20(3):848-863]	PubMed: 38250157
Lactylation drives hCG-triggered luteinization in hypoxic granulosa cells [ Int J Biol Macromol, 2024, 280(Pt 4):135580]	PubMed: 39322166
Dependence of NPPS creates a targetable vulnerability in RAS-mutant cancers [ Acta Pharmacol Sin, 2024, ]	PubMed: 39506063
Paeonol prevents sepsis-associated encephalopathy via regulating the HIF1A pathway in microglia [ Int Immunopharmacol, 2024, 143(Pt 1):113287]	PubMed: 39362015
Analysis of GCRV Pathogenesis and Therapeutic Measures Through Proteomic and Metabolomic Investigations in GCRV-Infected Tissues of Grass Carp (Ctenopharyngodon idella) [ Int J Mol Sci, 2024, 25(21)11852]	PubMed: 39519403
Hyperglycemia-induced Sirt3 downregulation increases microglial aerobic glycolysis and inflammation in diabetic neuropathic pain pathogenesis [ CNS Neurosci Ther, 2024, 30(8):e14913]	PubMed: 39123294
SUMO3 inhibition by butyric acid suppresses cell viability and glycolysis and promotes gemcitabine antitumor activity in pancreatic cancer [ Biol Direct, 2024, 19(1):74]	PubMed: 39183358
GDF15 ameliorates sepsis-induced lung injury via AMPK-mediated inhibition of glycolysis in alveolar macrophage [ Respir Res, 2024, 25(1):201]	PubMed: 38725041
Multi-omics and immunogenomics analysis revealed PFKFB3 as a targetable hallmark and mediates sunitinib resistance in papillary renal cell carcinoma: in silico study with laboratory verification [ Eur J Med Res, 2024, 29(1):236]	PubMed: 38622715

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人間や獣医の診断であるか治療的な使用のためにでない。

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