受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | NCI-C04831, Hydroxycarbamide,NSC-32065 | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
|
化学式 | CH4N2O2 |
|||
分子量 | 76.05 | CAS No. | 127-07-1 | |
Solubility (25°C)* | 体外 | DMSO | 15 mg/mL (197.23 mM) | |
Water | 15 mg/mL (197.23 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection. |
---|---|
in vitro | hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants[1][2].hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase[1]. |
細胞アッセイ | 細胞株 | Erythroid cells |
---|---|---|
濃度 | 30 μM | |
反応時間 | 96 h | |
実験の流れ | Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours. | |
動物実験 | 動物モデル | Female athymic (nu/nu) nude mice(used for xenograft model) |
投薬量 | 1500 mg/kg | |
投与方法 | i.p. |
Data from [Data independently produced by , , Blood, 2017, 130(26):2848-2859]
Data from [Data independently produced by , , Int J Biochem Cell Biol, 2018, 105:1-12]
TOPORS E3 ligase mediates resistance to hypomethylating agent cytotoxicity in acute myeloid leukemia cells [ Nat Commun, 2024, 15(1):7360] | PubMed: 39198401 |
Necrosulfonamide causes oxidation of PCM1 and impairs ciliogenesis and autophagy [ iScience, 2024, 27(4):109580] | PubMed: 38600973 |
CK2-dependent degradation of CBX3 dictates replication fork stalling and PARP inhibitor sensitivity [ Sci Adv, 2024, 10(21):eadk8908] | PubMed: 38781342 |
CDC5L surveils cellular stress responses and stress granule formation through transcriptional repression [ bioRxiv, 2024, 10.1101/2024.01.04.574133] | PubMed: none |
Extensive jejunal injury is repaired by migration and transdifferentiation of ileal enterocytes in zebrafish [ Cell Rep, 2023, 42(7):112660] | PubMed: 37342912 |
Targeting MCL-1 triggers DNA damage and an anti-proliferative response independent from apoptosis induction [ Cell Rep, 2023, 42(10):113176] | PubMed: 37773750 |
Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis [ Sci Adv, 2023, 9(4):eadf6277] | PubMed: 36706191 |
Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis [ Sci Adv, 2023, 9(4):eadf6277] | PubMed: 36706191 |
Genetic instability from a single S phase after whole-genome duplication [ Nature, 2022, 604(7904):146-151] | PubMed: 35355016 |
Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity [ Cell Res, 2022, 10.1038/s41422-022-00731-w] | PubMed: 36280710 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。